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Analysis method for organic phosphorus pesticide open hole visualized semi-quantitative detection

A technology for semi-quantitative detection of organophosphorus pesticides, applied in the field of quick test cards, can solve the problem of high cost and achieve the effects of low cost, good stability and good selectivity

Inactive Publication Date: 2017-08-18
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to complete an analysis process, two biological enzymes need to be used, and the cost of the method is high

Method used

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  • Analysis method for organic phosphorus pesticide open hole visualized semi-quantitative detection
  • Analysis method for organic phosphorus pesticide open hole visualized semi-quantitative detection
  • Analysis method for organic phosphorus pesticide open hole visualized semi-quantitative detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Synthesis of colorimetric reagent AuNRs and preparation of quick test card:

[0040] (1) The synthetic operation of AuNRs can be divided into two steps: the synthesis of gold species and the growth of gold nanorods.

[0041] (a) Synthesis of gold species: 250 μL of 0.01M HAuCl 4 ·3H 2 O was mixed with 9.75 mL of CTAB at a concentration of 0.1 M and 600 μL of freshly prepared NaBH was added 4 Solution (0.01M), after mixing uniformly for full reaction, place the obtained solution at 30° C. for 30 minutes to obtain stable gold species.

[0042] (b) Growth of AuNRs: Dissolve 0.9212g CTAB and 0.11g 5-bromosalicylic acid in 49mL water first, then add AgNO 3 (240μL / 0.02M), HAuCl 4 (500 μL / 0.05M) and AA (130 μL / 0.1M) three kinds of solutions, stirred vigorously to mix the solutions completely to obtain gold seed growth solution. Inject 80 μL of gold seed solution into the growth solution, stir and mix evenly, and let it stand at 30°C for 12 hours. The solution turns browni...

Embodiment 2

[0045] AuNRs in Au 3+ - Controlled etching in CTAB solution:

[0046] figure 2 and image 3 The prepared AuNRs were treated with different concentrations of Au 3+ UV-Vis absorption spectrum, solution color photo and transmission electron microscope picture after etching. Such as figure 2 As shown in A, the newly synthesized AuNRs have a longitudinal plasmon resonance absorption peak at 768 nm and a transverse plasmon resonance absorption peak at 514 nm. With Au 3+ With the increase of the concentration, the longitudinal plasmon peak is blue-shifted and the peak intensity decreases, while the transverse peak is slightly red-shifted. The ratio of the peak intensity of the longitudinal absorption peak to the intensity of the transverse absorption peak and the Au 3+ The concentration showed a good linear relationship ( figure 2 B), illustrating that by controlling the Au 3+ The concentration of AuNRs can be controlled to etch. With Au 3+ As the concentration increase...

Embodiment 3

[0048] Application of AuNRs in naked-eye visual semi-quantitative detection of organophosphorus pesticides:

[0049] Such as Figure 4 As shown, the newly synthesized AuNRs have strong longitudinal and weak transverse plasmon absorption peaks at 768 nm and 514 nm, respectively (curve a). Incorporation of AuNRs into Au-containing 3+ -In the solution of CTAB, the two absorption peaks of AuNRs disappeared (curve b), and the color of AuNRs solution also changed from brown to colorless, indicating that AuNRs were completely dissolved. When AChE and the substrate ATCh were added to this system, it was observed that the longitudinal absorption peak blue-shifted by about 10 nm (curve c). This is because the reduction product TCh produced by the enzymatic hydrolysis reaction consumes almost all of the Au 3+ , a small amount of residual Au 3+ The AuNRs were slightly etched so that the longitudinal absorption peak blue-shifted to 758nm, but the color of the solution was still brown a...

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Abstract

The invention provides an analysis method for an organic phosphorous pesticide open hole visualized semi-quantitative detection, and belongs to the technical field of rapid organic phosphorous pesticide detection. According to the analysis method, an AuNRs is used as the chromogenic reagent, wherein the AuNRs has an anisotropic surface plasmon resonance property, in the process of the length-diameter ratio of the AuNRs decreasing from 4.0 to 1.0, the AuNRs displays a series of observable color changes from brown to gray, green, blue, purple, pink and the like. Therefore, a relationship is established between the dissolving reaction of the AuNRs in an Au3+ solution and the pesticide inhibition and hydrolysis reaction of an AChE, and through the color changes of the AuNRs in the dissolving process, the semi-quantitative visualized detection of the organic phosphorus pesticide content can be achieved. The chromogenic reagent has a multicolor property, and every color is corresponding to a concentration of the organic phosphorus pesticide, so that the chromogenic reagent is applicable to the semi-quantitative visualized detection of the organic phosphorus pesticide in a certain range.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of organophosphorus pesticides, in particular to a colorimetric analysis method capable of semi-quantitative visual detection of organophosphorus pesticides, a rapid detection card and an application thereof. Background technique [0002] Organophosphorus pesticides (OPs) have become one of the most widely used pesticides in my country because of their broad-spectrum, high efficiency and easy degradation in the natural environment. The use of organophosphorus pesticides can effectively suppress pests and diseases and increase crop yields. However, the long-term use of organophosphorus pesticides has caused its residues in drinking water, soil, air and food, which has also caused a huge threat to the environment and human health. In order to avoid this hazard, many countries have issued national standards to strictly limit and regulate the use of organophosphorus pesticides. In this conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31
Inventor 吴硕李丹丹王加冕赵艳秋
Owner DALIAN UNIV OF TECH
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