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A kind of silk protein sponge and preparation method thereof

A technology of silk protein and sponge, which can be used in pharmaceutical formulations, prostheses, drug delivery, etc., and can solve problems such as unsuitable for soft tissue repair, inapplicability, and high mechanical properties

Active Publication Date: 2020-08-14
丝纳特(苏州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Researchers have developed a variety of methods for preparing silk protein sponges, mainly including salting-out method and freeze-drying method; the main conformation of the porous sponge prepared by salting-out method is beta-sheet crystal, which has high mechanical properties and is not suitable for soft tissue repair; Although the silk protein prepared by freeze-drying method is generally amorphous, it will dissolve in water, making it unusable. It can only be induced to form beta-sheet by alcohol treatment and vacuum water treatment, and finally obtain a water-insoluble silk protein. sponge material
Therefore, although there are various methods such as adding highly crystalline silk protein to induce, using acid to inhibit crystallization, etc. to reduce the crystallinity of the sponge, and to reduce the hardness of the sponge on the basis of maintaining the stability of the sponge in water, the water insolubility of the silk protein porous sponge, The inherent contradiction between mechanical properties and crystal structures still lacks solutions
On the other hand, in recent years, the introduction of nanofibrous structures imitating extracellular matrix has become an effective means to improve the biocompatibility of materials. At present, the method of adding nanofibers to induce the increase of the proportion of nanofibrous structures in silk protein sponges has been studied. , but completely composed of nanofibers, silk protein sponges with a composite structure still need continuous research

Method used

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  • A kind of silk protein sponge and preparation method thereof
  • A kind of silk protein sponge and preparation method thereof
  • A kind of silk protein sponge and preparation method thereof

Examples

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preparation example Construction

[0031] In order to obtain the silk protein sponge of the above structure, the application provides a preparation method of the silk protein sponge, comprising the following steps:

[0032] A), adjusting the concentration of the aqueous solution of amorphous silk protein nanofibers;

[0033] B), freezing the amorphous silk protein nanofiber solution obtained in step A);

[0034] C), freeze-drying the amorphous silk protein nanofibers obtained in step B) to obtain a silk protein sponge.

[0035] The present application utilizes metastable amorphous silk protein nanofibers to prepare amorphous nanofiber-microporous silk protein sponges through concentration adjustment-freezing-freeze-drying.

[0036] In the process of preparing silk protein sponge, the present application first adjusts the concentration of the aqueous solution of amorphous silk protein nanofibers, and in the process of adjusting the aqueous solution of amorphous silk protein nanofibers, the amorphous silk protei...

Embodiment 1

[0048] Embodiment 1 Preparation of amorphous silk protein nanofiber aqueous solution

[0049] (1) silk fiber is dissolved in lithium bromide-formic acid mixed solvent, the mass ratio of formic acid and lithium bromide in lithium bromide-formic acid mixed solvent is 1:13, and the concentration of lithium bromide is 9mol / L;

[0050] (2) Place the silk fiber solution dissolved in the above mixed solvent into a dialysis bag for dialysis for 72 hours to obtain an aqueous solution of amorphous silk protein nanofibers; the concentration of silk fibers in the silk fiber solution is 0.4 to 1 wt%, and the dialysis temperature is 4 to 1 wt%. between 25°C.

[0051] The amorphous fibroin nanofiber solutions in the following examples were all prepared from Example 1.

Embodiment 2

[0053] (1) Concentrate 50ml of the amorphous silk protein nanofiber solution with a concentration of 0.8%, at 2°C, use the method of PEO displacement concentration, concentrate for 36h, adjust the concentration to 4%, and inject it into the mold;

[0054] (2) The silk protein nanofiber solution injected into the mold is frozen at -4°C for 24 hours to form ice;

[0055] (3) The frozen material was placed in a lyophilizer, the temperature of the cold trap was -63° C., and lyophilized for 48 hours to obtain a water-insoluble silk protein sponge.

[0056] figure 1 and figure 2 Respectively the macroscopic picture and the microscopic picture of the amorphous silk protein nanofiber that step (1) adopts, image 3 For the infrared spectrogram of the amorphous silk protein nanofibers used in step (1), it can be seen from the figure that the amorphous silk protein nanofibers used in step (1) are in a solution state, with a fiber diameter of 10-20nm and a length of 100nm ~1μm, infrar...

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Abstract

The invention provides silk fibroin sponge and a preparation method thereof. The silk fibroin sponge is of an amorphous structure of a nanofiber-micropore composite structure, and is insoluble to water. The invention also provides a preparation method of the silk fibroin sponge, comprising the following steps: A) regulating the concentration of an amorphous silk fibroin nanofiber water solution; B) freezing the amorphous silk fibroin nanofiber water solution obtained by step A); C) freeze-drying amorphous silk fibroin nanofiber obtained by step B) to obtain the silk fibroin sponge. The porous silk fibroin sponge completely consisting of nanofiber and also having the amorphous structure is prepared by the steps. Different from an original silk fibroin sponge preparation method, the method has the advantages that when the sponge material is prepared, other treatment modes are absolutely not needed to induce crystallization for realizing the stability of the sponge material in water; the use of other inducers such as methyl alcohol and acid is avoided, the technology is simple and environment-friendly, and the biocompatibility is excellent.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a silk protein sponge and a preparation method thereof. Background technique [0002] Silk protein has attracted extensive attention in the field of biomedical materials due to its excellent biocompatibility, mechanical properties, degradability, easy shape, and ability to be processed in aqueous solution. Silk protein sponges with a porous structure have been widely used in laboratory research on different tissue repairs, and several products are undergoing clinical trials. Therefore, silk protein sponge materials are the most important components of silk protein-based biomaterials and have a wide range of applications. application prospects. [0003] Researchers have developed a variety of methods for preparing silk protein sponges, mainly including salting-out method and freeze-drying method; the main conformation of the porous sponge prepared by salting-out meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/56A61L27/58
CPCA61L27/227A61L27/56A61L27/58A61L2400/12A61L2430/34
Inventor 祝文莉吕强
Owner 丝纳特(苏州)生物科技有限公司