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Photo-thermal differential microscopic imaging apparatus and individual particle imaging method

A microscopic imaging and differential technology, applied in the direction of measuring devices, individual particle analysis, particle and sedimentation analysis, etc., can solve the problems of complex observation, damage, and influence of experimental observation

Active Publication Date: 2017-08-22
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In microscopy, photobleaching complicates observations because it disrupts the ability of fluorophores to continue to emit light, affecting experimental observations

Method used

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  • Photo-thermal differential microscopic imaging apparatus and individual particle imaging method
  • Photo-thermal differential microscopic imaging apparatus and individual particle imaging method
  • Photo-thermal differential microscopic imaging apparatus and individual particle imaging method

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Embodiment Construction

[0028] The content of the present invention and its application are explained below in conjunction with the accompanying drawings. The embodiments described are for better illustration of the present invention and should not be construed as limiting the claims of the present invention.

[0029] The specific working process is:

[0030] figure 1 It is a schematic diagram of a photothermal differential microscopic imaging device. The first laser 1 is a laser with a wavelength of 532nm. The laser light emitted by it enters the acousto-optic modulator 3 after being attenuated by an adjustable circular attenuation plate 2. The acousto-optic modulator 3 The modulation of the laser light intensity is realized under the signal frequency given by the function signal generator 30, and the modulated laser beam enters the laser beam expander 7 after passing through the aperture 4, the mirrors 5 and 6 in sequence, and passes through the laser beam expander 7 The effect of the beam will b...

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Abstract

The invention discloses a photo-thermal differential microscopic imaging apparatus and an individual particle imaging method. The apparatus comprises an illumination system for optical imaging, a pump-probe dualbeam weak signal detection system for extracting thermal absorption information surrounding metal particles, and a flat scanning system for two dimension scanning of a sample, wherein the illumination system for optical imaging is used for searching the position of a sample marker, the pump-probe dualbeam weak signal detection system is used for extracting thermal radiation weak signals surrounding the sample by using a detection light beam, and the flat scanning system is used for realizing two dimension scanning of the sample; extracted weak thermal signals are processed by a computer in order to product a two dimension intensity distribution map, and photo-thermal differential microscopic imaging of the individual particle is realized.

Description

technical field [0001] The invention relates to a system for realizing microscopic imaging of a single molecule, which realizes the imaging of a single particle by extracting a photothermal differential signal. Background technique [0002] Understanding the interaction between substances and the process of life in situ at the single-cell and single-molecule level has become a research hotspot in the field of life science today. Currently, single-molecule optical imaging techniques commonly used in the world include full-field phase-contrast microscopy, near-field optical scanning microscopy, confocal fluorescence microscopy, and total internal reflection fluorescence microscopy. These technologies have received extensive attention in the fields of molecular biology, molecular chemistry and nanomaterials. Among them, total internal reflection fluorescence microscopy uses the evanescent wave generated by total internal reflection to excite the sample. Since the intensity of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10
CPCG01N15/10
Inventor 方哲宇李博文朱星祖帅蒋瞧
Owner PEKING UNIV
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