Method for quantitatively detecting PSI-OAm-NAPI amphiphilic polymer nano-drug carriers based on trace fluorescence immunoassay

A nano-drug carrier and quantitative detection technology, which is applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems such as low sensitivity, reagent toxicity, and too strict requirements on the size of nanoparticles, and achieve detection costs. Low, high sensitivity, specific effect

Active Publication Date: 2017-08-25
ANHUI NORMAL UNIV
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Problems solved by technology

Electrochemical analysis has simple equipment, low price, and simple operation, but its stability is poor, its sensitivity is low, and the reagents have certain toxicity; mass spectrometry is widely used and efficient and fast, but the size requirements of nanoparticles are too strict; The counting method obtains the number of particles through the ultraviolet spectrum and calculation methods, and the accuracy of quantitative analysis is low; these methods cannot achieve the purpose of quantitatively detecting nanomaterials

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  • Method for quantitatively detecting PSI-OAm-NAPI amphiphilic polymer nano-drug carriers based on trace fluorescence immunoassay

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Embodiment 1

[0062] A method for quantitatively detecting PSI-OAm-NAPI amphiphilic polymeric nanometer drug carriers based on trace fluorescence immunoassay, said method comprising the following steps:

[0063] a, hydrolyzing PSI-OAm-NAPI to obtain a hydrolyzed MFAP solution;

[0064] Take 32mL of N,N-dimethylformamide and heat it to 60°C, then add 1.6g of polysuccinimide (PSI) and 1.63mL of oleylamine (OAm), react for 10min and add 0.83mL of N-(3-aminopropyl Base) imidazole (NAPI), and raise the temperature to 100°C for 5 hours, then cool to room temperature, add 160mL of methanol to make it evenly precipitate under magnetic stirring, take the precipitate and dry it after centrifugation, and then PSI-OAm-NAPI can be prepared (MFAP);

[0065] Dissolve 60 mg of MFAP nanoparticles and 0.6 mg of (MPEG-PLGA) in 1 mL of chloroform solution. After dissolving, add the above solution to 10 mL of sodium hydroxide solution with a concentration of 0.006 mg / mL, ultrasonic for 5 min, The power is abo...

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Abstract

The invention discloses a method for quantitatively detecting PSI-OAm-NAPI amphiphilic polymer nano-drug carriers based on trace fluorescence immunoassay. The method comprises the following steps: hydrolyzing PSI-OAm-NAPI to prepare and obtain immune antigens and coating antigens of MFAP, diluting the MFAP coating antigens through a coating solution and then coating the MFAP coating antigens in an elisa plate, sealing, adding MFAP standard products with different concentrations, taking FITC-labeled MFAP antibodies as primary antibodies, and building a direct competition fluorescence immunoassay quantitative detection MFAP. The method is capable of making full use of high specificity of the antigens and antibodies in the immunologic reaction and combining the high specificity of the antigens and the antibodies with sensibility of fluorescence, so that signals of the immunoreactions are further improved; the fluorescence intensity is used for carrying out quantitative analysis; the method is convenient and rapid, is low in detection cost, high in specificity and high in sensitivity, and is capable of carrying out high-flux measurement.

Description

technical field [0001] The invention relates to the field of quantitative detection of nanomaterials, in particular to a method for quantitative detection of PSI-OAm-NAPI amphiphilic polymeric nanometer drug carriers based on trace fluorescence immunity. Background technique [0002] Nanomaterials are a new class of materials in recent years. The development of nanotechnology has enabled nanomaterials to show their strengths in many fields; adding nanoscale silicon dioxide in the textile industry can be made into antibacterial, anti-mildew, deodorizing and anti-ultraviolet radiation Clothing; in household appliances, nano-functional plastics made of nano-materials have antibacterial, deodorizing, anti-corrosion, anti-aging, anti-ultraviolet and other effects; in the field of environmental science, nano-films can detect pollution, and these preparations are filtered to eliminate contamination. It can be seen that nanomaterials involve a wide range of fields, and they have al...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/533
CPCG01N21/6486G01N33/533
Inventor 张明翠闫希
Owner ANHUI NORMAL UNIV
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