A kind of tissue culture seedling propagation method of southern yew

Abstract

The invention discloses a taxus chinensis var tissue culture and seedling breeding method. According to the key point of the technical scheme, the method comprises the steps of selecting explants and putting the explants into a culture medium to perform cultivation, wherein the explants are subjected to disinfecting treatment after the explants are selected and before the explants are put into the culture medium; in the selected explants, the conduits are not developed maturely, are living cells and can perform differentiation and proliferation, and the wood parenchyma cannot achieve the effect of blocking differentiation of the explants. The explants are disinfected, so that the bacteria can be effectively removed from the surfaces of the explants and the possibility that the culture medium is polluted by the bacteria is reduced; meanwhile, the explants are smashed and the number of the broken cells can be reduced, so that the possibility that the explants are polluted by the bacteria is reduced; in the late-stage cultivation, the number of the bacteria is small, and the bacteria cannot pollute and damage the explants in the culture medium easily, so that normal proliferation of the explants is benefited, the success possibility of the experiment is improved, and mass commercialized production is benefited.

Description

technical field [0001] The invention relates to plant cultivation, in particular to a method for growing seedlings of the southern yew tissue culture. Background technique [0002] Taxus is a plant of the genus Taxus in the family Taxaceae. Paclitaxel is a complex secondary metabolite in Taxus plants, and it is the only drug that can promote microtubule polymerization and stabilize the polymerized microtubules. But the content of paclitaxel in the yew plant is very low, so the demand for yew in the market is high. [0003] The conventional propagation methods of yew plants are mainly cutting and sowing. However, the seed coat of yew seeds is thick and is in a deep dormant state. Under the natural state, it can only germinate after two winters and one summer, and the natural renewal ability is weak, which makes it difficult to sow and reproduce. Low, the survival rate of cuttings is low. Therefore, it is difficult to meet the large-scale production of yew by adopting trad...

AI Technical Summary

Problems solved by technology

[0005] This culture method is not sterilized. After the twigs or buds are mechanically crushed, some cells are crushed. During the constant temperature culture, the bacteria remaining without disinfection will use the broken cells as the mother to proliferate and pollute the medium.

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