Preparation method and application of skin dressing containing carrageenan/agarose

A technology of agarose and carrageenan, which is applied in medical science, absorbent pads, prostheses, etc., can solve problems such as property differences, and achieve the effects of low manufacturing cost, strong stability, and infection prevention

Inactive Publication Date: 2017-08-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the deficiencies and technical problems of existing skin dressings, and the problems existing in the application of commercial grade carrageenan, the primary purpose of the present invention is to provide a kind of skin dressing contain...

Method used

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  • Preparation method and application of skin dressing containing carrageenan/agarose
  • Preparation method and application of skin dressing containing carrageenan/agarose

Examples

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Embodiment 1

[0047] A skin dressing composite film containing λ-type carrageenan / agarose is a composite film composed of λ-type carrageenan and agarose in a mass ratio of 50:50.

[0048] The above-mentioned preparation method of the skin dressing composite film containing λ type carrageenan / agarose comprises the following steps:

[0049] (1) Prepare a commercial-grade λ-carrageenan solution with a mass fraction of 10%, heat it in a water bath at 90°C until it is completely dissolved, and filter it while it is hot. Add the same volume of 0.8mol / L KCl solution. Put it into a 50mL centrifuge tube and centrifuge at high speed (8000rpm, 10min), and take the supernatant into a clean beaker. Add twice the volume of absolute ethanol and decant. The precipitate was dialyzed in a dialysis bag with a molecular weight cut off of 3000 for three days, and concentrated by rotary evaporation. Place in a -20°C refrigerator and freeze overnight, then freeze-dry.

[0050] (2) Dissolving the agarose with ...

Embodiment 2

[0058] A skin dressing composite scaffold containing κ-type carrageenan / agarose is a composite scaffold material composed of κ-type carrageenan and agarose in a mass ratio of 40:60, and the tensile modulus is 0.292MPa.

[0059] The preparation method of the above-mentioned skin dressing composite support containing κ-type carrageenan / agarose comprises the following steps:

[0060] (1) Prepare a commercial grade κ-carrageenan solution with a mass fraction of 10%, place it in a water bath at 90°C to dissolve it by heating, and filter while it is hot. Add the same volume of 0.8mol / L KCl solution. Let stand at room temperature, decant and discard the supernatant. Put it into a dialysis bag with a molecular weight cut off of 3000 and dialyze for three days, then concentrate by rotary evaporation. Place in a -20°C freezer overnight and freeze dry.

[0061] (2) Dissolving the agarose with pure water at 90° C. to obtain an agarose solution with a mass fraction of 1%.

[0062] (3) ...

Embodiment 3

[0067] A skin dressing composite support containing iota-type carrageenan / agarose is a composite support material composed of iota-type carrageenan and agarose according to a mass ratio of 50:50, and the tensile modulus is 0.321MPa.

[0068] The preparation method of the above-mentioned skin dressing composite support containing iota carrageenan / agarose may further comprise the steps:

[0069] (1) Prepare a commercial grade iota-carrageenan solution with a mass fraction of 10%, place it in a water bath at 90° C. and heat until completely dissolved, and filter while it is hot. Add the same volume of 0.8mol / L KCl solution. Place in a 50mL centrifuge tube and centrifuge at high speed (8000rpm, 20min), discard the supernatant. The precipitate was dialyzed in a dialysis bag with a molecular weight cut off of 3000 for three days, and concentrated by rotary evaporation. Place in a -20°C freezer overnight and freeze dry.

[0070] (2) Dissolving the agarose with pure water at 90° C....

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Abstract

The invention discloses a preparation method and application of a skin dressing containing carrageenan/agarose. The skin wound dressing is prepared by blending the carrageenan and the agarose at a mass ratio of (10:90) to (50:50) and then directly drying to obtain a composite film or freeze-drying to obtain a composite scaffold with a three-dimensional porous layered structure; the carrageenan is a purified commercial grade carrageenan. A carrageenan purification method used in the invention solves the problems that the commercial grade carrageenan is insufficient in purity and the difference of the carrageenan between manufacturers is larger, so that a biological material with high stability is able to be prepared. The skin dressing prepared by the invention can provide certain nutrition for tissue healing, protect a surface of a wound resist virus and prevent infection during the tissue healing, and at the same time can regulate part functions of a body immune system in the process and really produce an effect of promoting the healing of surface of the wound.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a preparation method and application of a skin dressing containing carrageenan / agarose. Background technique [0002] Carrageenan, also known as carrageenan, is an anionic polysaccharide extracted from marine red algae and polymerized with linear sulfated galactose. Since its discovery by scientists in the 20th century, carrageenan has been widely used in texturing, thickening agents, and as a gel-forming component in the food and pharmaceutical industries. After years of development, carrageenan has become one of the world's top three alginates. According to the difference in its structure, the commercialized carrageenans are mainly κ-type, ι-type, and λ-type at present, among which the λ-type carrageenan has the highest degree of sulfated esterification. As early as 1972, the U.S. Food and Drugs Administration (Food And Drugs Administration) classified carrageenan group in...

Claims

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Application Information

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IPC IPC(8): A61L15/42A61L15/28A61L27/56A61L27/40A61L27/26A61L27/54
CPCA61L15/425A61L15/225A61L15/42A61L27/26A61L27/54A61L27/56A61L2300/232A61L2300/408A61L2300/412C08L5/00C08L5/12
Inventor 毛萱黄永睿汤顺清
Owner JINAN UNIVERSITY
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