Salmonella typhimurium detection biosensor
A biosensor, Salmonella technology, applied in the field of biosensors, can solve the problems of time-consuming and complicated instrument operation, and achieve the effects of improved sensitivity, good repeatability, and mild reaction conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0049] The preparation method of described biosensor comprises the following steps:
[0050] (1) Pretreatment of the electrodes;
[0051] (2) Modify the mixture of iDNA and Helper on the electrode surface;
[0052] (3) Modification of homogeneous reaction products onto the electrode surface.
[0053] In the preparation method, the operation steps of modifying the mixture of iDNA and Helper to the surface of the electrode are preferably as follows: add 10 μL of the mixture of iDNA and Helper dropwise to the pretreated electrode surface, and incubate at 37 °C for 2 h .
[0054] In the preparation method, the preferred steps of modifying the homogeneous reaction product onto the electrode surface are as follows:
[0055] (1) Add 14 μL of sterilized water, 2 μL of 10× buffer, 2 μL of 10 μM P1 and 2 μL of 10 μM P2 into a centrifuge tube, shake for 30 s, place in a thermostat at 90 °C for 10 min, and cool naturally to room temperature;
[0056] (2) Add 12 μL of sterilized water...
Embodiment 1
[0063] The main steps of the electrode modification process are as follows:
[0064] a. The gold electrode is first polished in 0.3 and 0.05 µm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;
[0065] b. Drop 10 μL of the mixture of iDNA and Helper (10 μM) onto the electrode surface, and incubate at 37 °C for 2 h. Fix the sulfhydryl chains to the electrode surface through Au-S bonds;
[0066] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:
[0067] a. Add sterilized water, 10× buffer, P1 and P2 (2 μM, 4 μM, 6 μM, 8 μM, 10 μM, 12 μM, 14mM) into the centrifuge tube, shake for 30 s, and place in 90 ℃ in an incubator for 10 min, and naturally cooled to room temperature;
[0068] b. Add sterilized water, 10× buffer, aptamer, P3 and the target object to be tested into the centrifuge tube, ...
Embodiment 2
[0079] The main steps of the electrode modification process are as follows:
[0080] a. The gold electrode is first polished in 0.3 and 0.05 µm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;
[0081] b. Drop 10 μL of the mixture of iDNA and Helper (10 μM) onto the electrode surface, and incubate at 37 °C for 2 h. Fix the sulfhydryl chains to the electrode surface through Au-S bonds;
[0082] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:
[0083] a. Add sterilized water, 10× buffer, P1 and P2 into the centrifuge tube, shake for 30 s, put it in a thermostat at 90 ℃ for 10 min, and cool down to room temperature naturally;
[0084] b. Add sterilized water, 10× buffer, aptamer, P3 (2 nM, 4 nM, 6 nM, 8 nM, 10 nM, 12 nM, 14 nM) and the target substance to be tested into the centrifuge tub...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap