Gene sequencing chip, gene sequencing system and sequencing method thereof

Abstract

The invention discloses a gene sequencing chip, a gene sequencing system and a sequencing method thereof. When the gene sequencing chip performs gene sequencing, after a sample gene and a reversible termination nucleotide are added to a micropore, the sample gene and the The reversible terminating nucleotides are paired to release hydrogen ions, and the Nernst potential is induced on the surface of the ion-sensitive membrane, and then an electric field is formed by applying a voltage to the transparent electrode layer, thereby controlling the switching layer to switch to the transmission state, and then according to the obtained switching layer The type of reversible terminating nucleotide corresponding to the light information in the transmission state determines the base type of the gene, realizing gene sequencing. The structure of the gene sequencing chip is simple and the production cost is low. The reversible terminating nucleotides used for paired sequencing do not need to be fluorescently labeled, and do not require optical systems such as backlight light source and laser light source. Gene sequencing can be realized only through the principle of natural light reflection. The sequencing method is simple and easy, greatly reducing the cost and time of gene sequencing.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a gene sequencing chip, a gene sequencing system and a sequencing method thereof. Background technique [0002] Gene sequencing technology is the most commonly used technology in modern molecular biology research. Since the development of the first generation of gene sequencing in 1977, gene sequencing technology has achieved considerable development. Among them, the development process of gene sequencing technology includes: the first-generation sanger sequencing technology, the second-generation high-throughput sequencing technology, the third-generation single-molecule sequencing technology, and the fourth-generation nanopore sequencing technology; the current mainstream sequencing technology in the market is still based on Second-generation high-throughput sequencing. [0003] The second-generation high-throughput sequencing technologies mainly include: Illumina's se...

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Problems solved by technology

[0003] The second-generation high-throughput sequencing technologies mainly include: Illumina's sequencing-by-synthesis technology, Thermo Fisher's ion semiconductor sequencing technology, ligation sequencing technology, and Roche's pyrosequencing technology; among them, Illumina's sequencing-by-synthesis method and Thermo Fisher's Fisher's ligation sequencing requires fluorescent labeling, as well as laser light source and optical system, which makes sequencing complicated and increases sequencing time and cost

Roche's pyrosequencing does not require a laser light source and optical system, but it also requires fluorescent labeling; and the sequencing device used in the ion semiconductor sequencing method needs to use a semiconductor manufacturing process to make an ion sensor and two field effect transistors, and its manufacturing process is complicated. , the production process is more difficult

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