Method for corresponding peanut genome chromosome sequence map to actual karyotype chromosome number

Abstract

The present invention discloses a method for corresponding a peanut genome chromosome sequence map to an actual karyotype chromosome number. According to the method, a peanut chromosome single copy oligonucleotide library is developed by using a peanut wild species A.duranensis genome sequence sketch, and the correspondence between the peanut genome chromosome sequence map and the actual karyotype chromosome number is achieved through probe labeling and sequential fluorescence in situ hybridization. According to the present invention, the correspondence between the sequence map of the A6 chromosome of the peanut wild species A.duranensis genome and the chromosome having the actual karyotype chromosome number of A3 is firstly achieved, such that the foundation is established for the integration of peanut genome information, chromosome labeling information and genetic linkage map information so as to develop the new approach for the revealing of the peanut chromosome exchange, recombination and evolution law; and the method can be used for the development of peanut breeding research, can establish the foundation for the construction of the peanut physical map and the physical locating of the single gene, and can promote the research and utilization of the gene.

Description

technical field [0001] The invention relates to a peanut genome research method, in particular to a method for corresponding the peanut genome chromosome sequence diagram to the actual karyotype chromosome sequence number. Background technique [0002] Peanut is an important oil crop and economic crop in the world, and is an important source of oil and protein in many developing countries. China is the world's largest peanut producer, consumer and exporter. Therefore, improving the yield, quality and resistance of peanut is of great significance to ensure the safe supply of edible vegetable oil in my country and improve people's living standards. Studies have shown that improved varieties contribute more than 40% to crop yield increases. Therefore, breeding improved varieties, especially those that combine important traits such as high yield, high quality, and disease resistance, is particularly important for the development of the peanut industry. [0003] The peanut cult...

AI Technical Summary

Problems solved by technology

The chromosome sequence diagram does not correspond to the chromosome karyotype sequence number, resulting in unclear physical locations of BAC and annotated genes on the chromosome, making genome research and excellent gene utilization difficult

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