Method for corresponding peanut genome chromosome sequence map to actual karyotype chromosome number

A chromosome and genome technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficulty in using excellent genes in genome research, unclear physical locations, etc., and achieve the effect of promoting research and utilization

Active Publication Date: 2017-09-05
HENAN ACAD OF AGRI SCI
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Problems solved by technology

The chromosome sequence diagram does not correspond to the chromosome karyotype sequence number, resulting in unclear physi

Method used

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  • Method for corresponding peanut genome chromosome sequence map to actual karyotype chromosome number
  • Method for corresponding peanut genome chromosome sequence map to actual karyotype chromosome number
  • Method for corresponding peanut genome chromosome sequence map to actual karyotype chromosome number

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Embodiment

[0053] A method for corresponding the peanut genome chromosome sequence diagram to the actual karyotype chromosome sequence number, comprising the following steps:

[0054] (1) Design of chromosome-specific oligonucleotide (oligo) probes

[0055] Using the draft genome sequence of the peanut wild species A. duranensis published by PeanutBase (https: / / peanutbase.org / gbrowse_aradu1.0), select the 0-8.5 Mb end of the short arm of the gene-enriched region A6 as the target segment. Then design analysis parameters: (a) oligonucleotide length 42-48nt; (b) oligonucleotide density>2oligos / kb; (c) oligonucleotide dTm (DNA melting temperature - hairpin DNA melting temperature)> 10°C; the genome sequence and analysis parameters of the target segment were sent to MYcroarray Company (USA) for bioinformatics analysis, and MYcroarray Company used the RepeatMasker software to analyze the target segment and eliminate the genome repeat sequence of the target segment (http: / / www. repeatmasker.or...

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Abstract

The present invention discloses a method for corresponding a peanut genome chromosome sequence map to an actual karyotype chromosome number. According to the method, a peanut chromosome single copy oligonucleotide library is developed by using a peanut wild species A.duranensis genome sequence sketch, and the correspondence between the peanut genome chromosome sequence map and the actual karyotype chromosome number is achieved through probe labeling and sequential fluorescence in situ hybridization. According to the present invention, the correspondence between the sequence map of the A6 chromosome of the peanut wild species A.duranensis genome and the chromosome having the actual karyotype chromosome number of A3 is firstly achieved, such that the foundation is established for the integration of peanut genome information, chromosome labeling information and genetic linkage map information so as to develop the new approach for the revealing of the peanut chromosome exchange, recombination and evolution law; and the method can be used for the development of peanut breeding research, can establish the foundation for the construction of the peanut physical map and the physical locating of the single gene, and can promote the research and utilization of the gene.

Description

technical field [0001] The invention relates to a peanut genome research method, in particular to a method for corresponding the peanut genome chromosome sequence diagram to the actual karyotype chromosome sequence number. Background technique [0002] Peanut is an important oil crop and economic crop in the world, and is an important source of oil and protein in many developing countries. China is the world's largest peanut producer, consumer and exporter. Therefore, improving the yield, quality and resistance of peanut is of great significance to ensure the safe supply of edible vegetable oil in my country and improve people's living standards. Studies have shown that improved varieties contribute more than 40% to crop yield increases. Therefore, breeding improved varieties, especially those that combine important traits such as high yield, high quality, and disease resistance, is particularly important for the development of the peanut industry. [0003] The peanut cult...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6841C12Q2563/107
Inventor 张新友杜培李丽娜刘华秦利付留洋董文召黄冰艳汤丰收
Owner HENAN ACAD OF AGRI SCI
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