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An antagonistic endophytic bacterium gh011 and its application

An endogenous bacteria and antagonism technology, applied in the field of microorganisms, can solve the problems of decreased control effect of chemical agents, endangering human and animal health, and pollution of agricultural products and the environment by chemical agents

Inactive Publication Date: 2020-07-07
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term and continuous use of drugs has made Botrytis cinerea resistant, leading to a decline in the control effect of chemical agents year by year, and chemical agent residues seriously pollute agricultural products and the environment, endangering the health of humans and animals

Method used

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  • An antagonistic endophytic bacterium gh011 and its application
  • An antagonistic endophytic bacterium gh011 and its application
  • An antagonistic endophytic bacterium gh011 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Potted tomato control using preparations to prevent tomato gray mold:

[0019] Step 1. Inoculate a single colony of GH011 strain in 200mL LB liquid medium, in a constant temperature shaking incubator at 28°C at 160 r min -1 After culturing for 24 h, the cultured bacterial solution was diluted to 4×10 8 cfu mL – 1 , preparing a preparation for preventing tomato gray mold;

[0020] Step 2: Take potted tomatoes that grow to 5-6 leaves and have the same growth, spray the front and back of the tomato leaves with the preparation for treating and preventing tomato gray mold, and use clean water as a control. After 24 hours, the spore suspension of tomato gray mold 1×10 7 spores / mL (Pour a little sterile water into the PDA plate covered with Botrytis cinerea, scrape off the spores on the surface of the agar, pour them into a sterile container, shake them fully, filter them with sterile gauze, and count them on the blood cell plate) spray inoculated on it. Tomato plants we...

Embodiment 2

[0026] Screening, isolation and purification of antagonistic endophytic bacteria GH011 strain:

[0027] Step 1, preparation of culture medium:

[0028] LB medium for bacterial plate culture (tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar powder 18g / L), LB liquid medium for liquid culture (without adding agar powder), fungi PDA medium (potato 200g / L; glucose 20g / L; agar powder 18g / L) was used for culture and plate confrontation test.

[0029] Step 2: Collect 3g leaves of healthy sweet-scented osmanthus plants, soak in 75% alcohol for 30s, then disinfect with 1% sodium hypochlorite for 3min, rinse with sterile distilled water for 5 times, take the last rinse solution 200μL, and spread on LB On the culture medium plate; the sterilized leaves were ground in a sterile mortar, mixed with 5ml of sterile distilled water, and 200 μL of the grinding liquid was taken and spread on the LB medium plate. Cultivate at 28°C and observe day by day. If there is no colony in t...

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Abstract

The present invention relates to an antagonistic endophytic bacterium GH011, preservation number: M 2017096; the antagonistic endophytic bacterium GH011 can be used to inhibit Osmanthus fragrans leaf spot and tomato Botrytis cinerea; it can be used to prepare preparations for treating and preventing tomato Botrytis cinerea, The preparation method is to inoculate a single colony of the antagonistic endophytic bacterium GH011 in LB liquid medium, and place it in a constant temperature shaking incubator at 28°C for 160 r min ‑1 Cultivate for 24 h, and use sterile water to dilute the colonies of the cultured bacterial solution to 4×10 8 cfu mL –1 . The control method is to spray the tomato plants with the culture solution of the antagonistic endophytic bacteria GH011; the anti-endophytic bacteria GH011 of the present invention can effectively prevent and treat Botrytis cinerea, and the control effect reaches 64.9%, and the bacterium is effective against Osmanthus osmanthus leaf spot ( Staphylococcus aureus) had a bacteriostatic rate of 83.69%.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an antagonizing endophytic bacterium GH011 and its application. Background technique [0002] Tomato gray mold has become the main disease of tomato production in my country's protected areas. Botrytis cinerea is caused by the fungus Botrytis cinerea. At present, there is no breakthrough in tomato disease-resistant breeding, and domestic control of tomato gray mold mainly relies on chemical agents. However, long-term and continuous drug use has made Botrytis cinerea resistant, leading to a decline in the control effect of chemical agents year by year, and chemical agent residues seriously pollute agricultural products and the environment, endangering the health of humans and animals. Biological control has become an important means of disease control, and plant endophytic bacteria have unique advantages as potential resource bacteria for biological control of plant diseases, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 李永丽周洲李红英闫作炳王少博林乾坤王亚红
Owner HENAN UNIV OF SCI & TECH
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