Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum

The invention belongs to the technical field of micro-spectral imaging detection and relates to a method and device for testing a spectral pupil laser differential confocal CARS micro-spectrum. The core thought is as follows: Rayleigh light and CARS light loaded with tested sample spectral characteristics are excited by double lasers as a light source; and nondestructive separation is carried out on the Rayleigh light and the CARS light by using a dichroic optical system, wherein the Rayleigh light is subjected to geometrical detection and positioning, and the CARS light is subjected to spectrum detection. By using the characteristic that a zero crossing point of the spectral pupil laser differential confocal curve accurately corresponds to a focus position, the focus position of an excitation beam is accurately captured and positioned, and high-precision geometrical detection and high-spatial-resolution spectrum detection are achieved to form the method and device capable of achieving high-spatial-resolution spectrum detection of a sample micro-region. Through combining a CARS microscopical technique, the excited Raman scattering light loaded with sample information is shorter than a traditional spontaneous Raman effect in time, and the sample can be quickly subjected to nondestructive detection. The method and the device have the advantages of accurate positioning, high spatial resolution, nondestructive detection and high spectrum detection sensitivity, and a new way is provided for micro-region spectrum detection and geometrical measurement.