Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum
A differential confocal and spectral testing technology, used in materials excitation analysis and other directions, can solve the problems of limiting the ability of CARS microscopy system to detect micro-region spectra, restricting applications, etc., to improve the lateral resolution of the system and improve the signal-to-noise ratio. Effect
Inactive Publication Date: 2017-09-15
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF5 Cites 11 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0006] The above reasons limit the ability of the CARS microscope system to detect micro-region spectra, and restrict its application in finer micro-region spectrum testing and analysis occasions
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0058] In this embodiment, a picosecond laser with a wavelength of 532nm is used as the pump light source and a probe light source, and a supercontinuum picosecond laser with the same repetition rate is used as the Stokes light source with a bandpass filter of 550-650nm , mixed and emitted under the conditions of space coincidence and time consistency, after the structured beam is obtained through the pupil filter, the illumination pupil and the measurement objective lens are tightly focused on the sample, and the phase matching condition is satisfied at this time, and the excitation wavelength range is 450 Anti-Stokes light (CARS) at ~515 nm and Rayleigh light at 532 nm.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the technical field of micro-spectral imaging detection and relates to a method and device for testing a spectral pupil laser differential confocal CARS micro-spectrum. The core thought is as follows: Rayleigh light and CARS light loaded with tested sample spectral characteristics are excited by double lasers as a light source; and nondestructive separation is carried out on the Rayleigh light and the CARS light by using a dichroic optical system, wherein the Rayleigh light is subjected to geometrical detection and positioning, and the CARS light is subjected to spectrum detection. By using the characteristic that a zero crossing point of the spectral pupil laser differential confocal curve accurately corresponds to a focus position, the focus position of an excitation beam is accurately captured and positioned, and high-precision geometrical detection and high-spatial-resolution spectrum detection are achieved to form the method and device capable of achieving high-spatial-resolution spectrum detection of a sample micro-region. Through combining a CARS microscopical technique, the excited Raman scattering light loaded with sample information is shorter than a traditional spontaneous Raman effect in time, and the sample can be quickly subjected to nondestructive detection. The method and the device have the advantages of accurate positioning, high spatial resolution, nondestructive detection and high spectrum detection sensitivity, and a new way is provided for micro-region spectrum detection and geometrical measurement.
Description
technical field [0001] The invention belongs to the technical field of microspectral imaging, and relates to a split-pupil laser differential confocal CARS microspectral testing method and device, which can be used to quickly detect micro-area anti-Stokes scattering (CARS) spectra of various samples, High spatial resolution imaging and detection can be realized. technical background [0002] Optical microscopes are widely used in the fields of biomedicine and material science. With the rapid development of modern science, the requirements for microscopic imaging have shifted from structural imaging to functional imaging. In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographic imaging characteristics of confocal microscopy, and has the ability of n...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N21/63
CPCG01N21/63
Inventor 赵维谦吴寒旭邱丽荣王允
Owner BEIJING INSTITUTE OF TECHNOLOGYGY