Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum

A differential confocal and spectral testing technology, used in materials excitation analysis and other directions, can solve the problems of limiting the ability of CARS microscopy system to detect micro-region spectra, restricting applications, etc., to improve the lateral resolution of the system and improve the signal-to-noise ratio. Effect

Inactive Publication Date: 2017-09-15
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

[0006] The above reasons limit the ability of the CARS microscope system to detect micro-region s...
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Abstract

The invention belongs to the technical field of micro-spectral imaging detection and relates to a method and device for testing a spectral pupil laser differential confocal CARS micro-spectrum. The core thought is as follows: Rayleigh light and CARS light loaded with tested sample spectral characteristics are excited by double lasers as a light source; and nondestructive separation is carried out on the Rayleigh light and the CARS light by using a dichroic optical system, wherein the Rayleigh light is subjected to geometrical detection and positioning, and the CARS light is subjected to spectrum detection. By using the characteristic that a zero crossing point of the spectral pupil laser differential confocal curve accurately corresponds to a focus position, the focus position of an excitation beam is accurately captured and positioned, and high-precision geometrical detection and high-spatial-resolution spectrum detection are achieved to form the method and device capable of achieving high-spatial-resolution spectrum detection of a sample micro-region. Through combining a CARS microscopical technique, the excited Raman scattering light loaded with sample information is shorter than a traditional spontaneous Raman effect in time, and the sample can be quickly subjected to nondestructive detection. The method and the device have the advantages of accurate positioning, high spatial resolution, nondestructive detection and high spectrum detection sensitivity, and a new way is provided for micro-region spectrum detection and geometrical measurement.

Application Domain

Technology Topic

PhysicsSpectral imaging +11

Image

  • Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum
  • Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum
  • Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum

Examples

  • Experimental program(1)
  • Effect test(1)

Example Embodiment

[0057] Example
[0058] In this embodiment, a picosecond laser with a wavelength of 532nm is used as the pump light source and detection light source, and a supercontinuum picosecond laser with the same repetition frequency is used with a 550-650nm bandpass filter as the Stokes light source. , Mixing and exiting under the conditions of space coincidence and time consistency. After the structured light beam is obtained through the pupil filter, the illumination pupil and the measurement objective lens are tightly focused on the sample. At this time, the phase matching condition is met, and the excitation wavelength range is 450 ~515nm anti-Stokes light (CARS) and Rayleigh light with a wavelength of 532nm.
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Description & Claims & Application Information

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