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Fast detection kit for detecting PD-L1 and detection method of fast detection kit

A detection kit, PD-L1 technology, applied in the field of medical testing, can solve the problems of low sensitivity and low specificity, and achieve the effect of stable repeatability and reliability

Active Publication Date: 2017-09-22
尼沃诺斯(苏州)生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing kits for detecting PD-L1 have disadvantages such as low sensitivity and low specificity.

Method used

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  • Fast detection kit for detecting PD-L1 and detection method of fast detection kit
  • Fast detection kit for detecting PD-L1 and detection method of fast detection kit
  • Fast detection kit for detecting PD-L1 and detection method of fast detection kit

Examples

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Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Composition and preparation of the rapid detection kit for detecting PD-L1 of the present invention

[0055] The test kit of the present invention comprises the following components:

[0056] (1) Luminescent Substrate Solution I; (2) Luminescent Substrate Solution II; (3) Concentrated Washing Solution; (4) PD-L1 Standard; (5) PD-L1 Quality Control; (6) Antibody Acridine Ester tags and (7) magnetic bead immune complexes.

[0057] The preparation of each reagent in the kit of the present invention comprises the following steps:

[0058] (1) Preparation of luminescent substrate solution I:

[0059] Preparation method: Prepare dilute nitric acid solution with a concentration of 0.1M with double distilled water and nitric acid, and add hydrogen peroxide to make the concentration of hydrogen peroxide 0.08M.

[0060] (2) Preparation of luminescent substrate solution II:

[0061] Preparation method: prepare a 0.2M sodium hydroxide solution with double distilled w...

Embodiment 2

[0094] Example 2. Using the rapid detection kit for detecting PD-L1 of the present invention to detect PD-L1

[0095] S1 Pretreat the sample to be tested, take the supernatant, and equilibrate at 25°C for 10 minutes;

[0096] S2 Add 25 μL of PD-L1 calibrator, PD-L1 quality control substance or test sample to each flat-bottomed test tube, incubate at 37°C for 10 minutes, add 25 μL of magnetic bead immune complex, mix well, and incubate at 37°C for 10 minutes ;

[0097] S3 Place the flat-bottomed test tube on the magnetic separator for 3 minutes, remove the supernatant, invert the flat-bottomed test tube and the magnetic separator together on absorbent paper and pat for 3 times to completely remove the liquid in the hole. Add 200 μL of concentrated washing solution to a flat-bottomed test tube, mix well, put the flat-bottomed test tube on the magnetic separator for 3 minutes, remove the supernatant, put the test tube and the magnetic separator upside down on absorbent paper Dr...

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Abstract

The invention belongs to the technical field of medical examination, and particularly relates to a fast detection kit for detecting PD-L1 and a detection method of the fast detection kit. The fast detection kit for detecting the PD-L1 comprises a luminous substrate solution I, a luminous substrate solution II, a concentrated detergent, a PD-L1 standard product, a PD-L1 quality control product, an antibody acridinium ester marker and a magnetic bead immune complex. The fast detection kit for detecting the PD-L1 has the advantages of short detection time, small use amount, high efficiency, high sensitivity, high specificity, stable repetitiveness and reliability and the like.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a rapid detection kit for detecting PD-L1 and a detection method thereof. Background technique [0002] The full name of PD-1 is programmed death receptor 1, and its English name is programmed death 1. It is an important immunosuppressive molecule and a member of the CD28 superfamily. Immune regulation targeting PD-1 is of great significance in anti-tumor, anti-infection, anti-autoimmune diseases, and organ transplant survival. Its ligand PD-L1 can also be used as a target, and the corresponding antibody can also play the same role. The full name of PD-L1 is programmed death-ligand 1, and its English name is programmed cell death-Ligand 1. It is the first type of transmembrane protein with a size of 40kDa. Under normal circumstances, the immune system will respond to foreign antigens accumulated in the lymph nodes or spleen, promoting the proliferation of an...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/553G01N33/532G01N33/531
CPCG01N33/531G01N33/532G01N33/54326G01N33/553
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner 尼沃诺斯(苏州)生物工程有限公司