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A Method for Establishing a Cell Cycle Scattering Light Intensity Model Using Polystyrene Balls

A technology of polystyrene balls and cell cycle, applied in the measurement of scattering characteristics, etc., can solve problems such as difficult, difficult to survive, and constantly changing cell cycle, and achieve the effects of stable properties, good universality, and good technical application prospects

Active Publication Date: 2019-07-05
CHONGQING UNIV
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Problems solved by technology

However, since it is difficult for living biological cells to survive permanently in a culture vessel, and biological cells will continue to divide and grow during their survival, resulting in constant changes in the cell cycle, it is difficult to use biological cells to make and preserve the scattered light of each fixed cell cycle Strong detection reference standard
There is currently no other effective method for building a model of cell cycle scattered light intensity

Method used

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  • A Method for Establishing a Cell Cycle Scattering Light Intensity Model Using Polystyrene Balls
  • A Method for Establishing a Cell Cycle Scattering Light Intensity Model Using Polystyrene Balls
  • A Method for Establishing a Cell Cycle Scattering Light Intensity Model Using Polystyrene Balls

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Embodiment

[0038] Adopt the method of the present invention, set up the cell cycle scattered light intensity model of HeLa cell, specifically comprise the following steps:

[0039] 1) The pre-made specified concentration is 10 5 polystyrene spheres / mL suspensions of polystyrene spheres with different particle sizes were put into glass test tubes, shaken up separately, and measured by wide-angle light scattering instrument at 0 The scattered light intensity at intervals of 5° in the range of ~180° uses a laser as the light source, and the laser wavelength of the laser is 532nm. According to the measurement results, the corresponding scattered light intensity of the suspensions of polystyrene spheres with different particle sizes is obtained distribution curve.

[0040] 2) Use HeLa cell samples in G1 phase, S phase, G2 phase and M phase, respectively press 10 5 HeLa cell suspensions corresponding to G1 phase, S phase, G2 phase and M phase were prepared at the specified concentration of 1...

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Abstract

The invention provides a method for establishing a cell cycle scattering light intensity model by using polystyrene spheres. In the method, a suspension containing polystyrene spheres at different grain sizes is used to establish the cell cycle scattering light intensity model, the cell cycle scattering light intensity model is used as a reference standard for the detection of intensity of scattering light of the cell cycle of a living creature, the property of the polystyrene sphere is stable, the constant change of the cell cycle caused by cell division and growth of a cell is not produced, so that the cell cycle scattering light intensity model can be stored permanently, and the problem that the permanently stored reference standard for the detection of intensity of scattering light of the cell cycle in the prior art is difficult to store is solved; and in addition, when the polystyrene spheres at different grain sizes are diluted into concentrations of different gradients, the angle corresponds to the scattering properties of light at different time phases in the cell cycle or corresponds to the scattering properties of light at different time phases in the cell cycle after modification according to the scattering properties of light, and therefore, the cell cycle scattering light intensity model established by the method is excellent in universality, and an extensible concept is provided for improving more cell cycle scattering light intensity models in the future.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to cell detection technology, in particular to a method for establishing a cell cycle scattered light intensity model using polystyrene balls. Background technique [0002] The cell cycle refers to the whole process that cells go through from the completion of one division to the end of the next division, which is divided into two stages: interphase and division phase. Among them, the interphase is divided into three phases, namely, the early stage of DNA synthesis (G1 phase), the DNA synthesis phase (S phase) and the late DNA synthesis phase (G2 phase). The division phase (M phase) is divided into early, middle, late and telophase. [0003] Cell cycle theory not only provides an important theoretical basis for tumor research, but also cell cycle is the theoretical basis of tumor chemotherapy. Precise anti-tumor drugs can directly kill cancer cells in different cycles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/47
CPCG01N21/47
Inventor 林晓钢翁凌冬宛楠朱濠
Owner CHONGQING UNIV
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