Method for separating and purifying moxidectin

Abstract

The invention discloses a method for separating and purifying moxidectin. The method comprises the step of carrying out separation and purification to obtain the moxidectin of which the purity is 96% or above, wherein a supercritical fluid chromatographic system is adopted, a silica-gel-based filler is used as a stationary phase, supercritical CO2 is used as a mobile phase, and a mixture of methanol, ethanol and acetonitrile is used as an entrainer. The method for separating and purifying moxidectin is simple to operate; and more importantly, by using the supercritical CO2 as the mobile phase, only small amounts of organic solvents are added, thereby reducing the environmental pollution caused by organic solvents, and being green and environment-friendly.

Description

technical field

[0001] The invention relates to a method for separating and purifying macrolide antibiotics, in particular to a method for purifying moxidectin. Background technique

[0002] Moxidectin, alias moxidectin, English name is Moxidectin, white or off-white amorphous powder, almost insoluble in water, very soluble in ethanol (96%), slightly soluble in hexane, is a new type of anti Parasite medicine is a single-component macrolide antibiotic fermented by Streptomyces.

[0003] Compared with other macrolide antiparasitic drugs such as ivermectin and avermectin, moxidectin has a single component, and has higher anthelmintic activity, long-term, and safety characteristics, especially for nematodes (adult worms) and larvae) and arthropods, it has good anti-insect activity at extremely low doses, and has high repelling activity against nematodes and arthropod parasites in dogs, cattle, sheep, and horses, and has been widely used to treat dairy cows, Internal and extern...