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Selenium-enriched tea protein polypeptide and use thereof

A technology for selenium-enriched tea and protein peptides, which is applied in the field of protein peptides, can solve the problems that tea protein cannot be used well, it is difficult to use health drinks, and the water solubility of protein is reduced, so as to achieve good health, rich nutrition, and low water solubility. Extract convenient effects

Inactive Publication Date: 2017-10-03
深圳知本康业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Most of the proteins in tea are water-insoluble, only 1%-2% are water-soluble, and in the process of making tea, due to the denaturation and solidification of proteins, the water-solubility of some proteins is further reduced;
[0005] (2) Due to the strong resistance of tea protein, poor water solubility, and difficulty in purification, tea protein cannot be well utilized, especially in health drinks

Method used

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  • Selenium-enriched tea protein polypeptide and use thereof
  • Selenium-enriched tea protein polypeptide and use thereof
  • Selenium-enriched tea protein polypeptide and use thereof

Examples

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preparation example Construction

[0033] A selenium-enriched tea protein polypeptide, the preparation method of which comprises:

[0034] Pretreatment step: take fresh selenium-enriched tea leaves, wash them to remove impurities, degrease and dry them, and crush them to 10-30 mesh to obtain selenium-enriched tea leaves powder;

[0035] Protein modification step: at room temperature, add pure water and stabilizer to the selenium-enriched tea powder in sequence, the solid-liquid ratio of selenium-enriched tea powder, stabilizer and purified water is 1: (0.1-0.2): (15-17) , adjust the pH to 6-7, then microwave heating to 50-60°C, keep warm at this temperature for 1-2h, and filter to obtain the crude selenium-enriched tea filtrate;

[0036] One enzymatic hydrolysis step: after the selenium-enriched tea filtrate drops to normal temperature, add sodium hydroxide solution to adjust the pH to 9-10, add alkaline protease, selenium-enriched tea filtrate, sodium hydroxide to the selenium-enriched tea filtrate The volume...

Embodiment 1

[0049] A selenium-enriched tea protein polypeptide, the preparation method of which comprises:

[0050] Pretreatment step: take fresh selenium-enriched tea leaves, wash them to remove impurities, degrease and dry them, and crush them to 20 meshes to obtain selenium-enriched tea leaves powder;

[0051] Protein modification step: at room temperature, add pure water and stabilizer to the selenium-enriched tea powder in sequence, the solid-liquid ratio of selenium-enriched tea powder, sodium bisulfite and purified water is 1:0.1:16, adjust the pH to 6, Then microwave-heated to 60°C, kept at this temperature for 1h, and filtered to obtain the coarse filtrate of selenium-enriched tea leaves;

[0052] One enzymatic hydrolysis step: after the selenium-enriched tea filtrate drops to normal temperature, add sodium hydroxide solution to adjust the pH to 9, add alkaline protease, selenium-enriched tea filtrate, sodium hydroxide solution and The volume ratio of alkaline protease is 1:12:0.0...

Embodiment 2

[0058] A selenium-enriched tea protein polypeptide, the preparation method of which comprises:

[0059] Pretreatment step: taking fresh selenium-enriched tea leaves, washing to remove impurities, degreasing and drying, and crushing to 10 meshes to obtain selenium-enriched tea leaves powder;

[0060] Protein modification step: at room temperature, add pure water and stabilizer to the selenium-enriched tea powder in sequence, the solid-liquid ratio of selenium-enriched tea powder, sodium bisulfite and purified water is 1:0.1:17, adjust the pH to 7, Then microwave heating to 50° C., keep warm at this temperature for 2 hours, and filter to obtain the selenium-enriched tea coarse filtrate;

[0061] One enzymatic hydrolysis step: after the selenium-enriched tea filtrate drops to normal temperature, add sodium hydroxide solution to adjust the pH to 9-10, add alkaline protease, selenium-enriched tea filtrate, sodium hydroxide to the selenium-enriched tea filtrate The volume ratio of ...

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Abstract

The invention discloses a selenium-enriched tea protein polypeptide. A preparation method of the selenium-enriched tea protein polypeptide comprises pretreatment, protein modification, primary enzymatic hydrolysis, secondary enzymatic hydrolysis, enzyme inactivation and concentration. The invention also provides a use of the selenium-enriched tea protein polypeptide in the preparation of a health drink. The protein polypeptide is prepared by from selenium-enriched tea as a raw material through protein modification, two-step enzymolysis, enzyme inactivation and concentration. The selenium-enriched tea protein polypeptide is easy to extract, has a high extraction yield, is suitable for the human body and has good health care effects.

Description

technical field [0001] The invention relates to a protein polypeptide, in particular to a selenium-enriched tea protein polypeptide and its application. Background technique [0002] Tea trees mainly grow in warm and humid climates between 16°-30° north latitude. The optimum temperature for production is 20-25°C. It is recorded that "tea as a drink originated from Shennong." People mainly dry the tea leaves, brew them with boiling water, and then drink them. According to research reports, tea can reduce the risk of cardiovascular and cerebrovascular diseases and death, lower cholesterol and blood pressure, help prevent and treat early senile dementia, and lose weight. However, due to the presence of ester catechins in tea, tea leaves Strong bitterness and astringency, such as common black tea, yellow tea, black tea, oolong tea, etc., the taste is bitter and astringent. During the traditional tea fermentation process, the main reason is that the polyphenols in the tea have u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12G3/04
CPCC12G3/04C12P21/06
Inventor 不公告发明人
Owner 深圳知本康业有限公司