Application of Shewanella oneidensis and immobilized sphere thereof in treatment of printing and dyeing wastewater
A technology of Shewanella Oneida and immobilized pellets, which is applied in the direction of immobilizing on or in inorganic carriers, fixing on/in organic carriers, textile industry wastewater treatment, etc., to improve the recycling rate , Improve the effect of decolorization efficiency
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Embodiment 1
[0044] Example 1 Preparation of Immobilized Beads of Shewanella Oneida
[0045] A preparation method for Shewanella Oneida immobilized pellets, comprising the following steps:
[0046](1) Weigh 8g of polyvinyl alcohol (the average degree of polymerization is 1800±100) and add it to 100mL of deionized water. After soaking for 30 minutes, stir it in a water bath at 80-90°C until it is completely dissolved, and then add 0.5g of seaweed into the solution. Na2SO4, stirred, mixed evenly to obtain PVA-SA suspension;
[0047] (2) Pick a single colony of Shewanella Oneida and inoculate it in 6mL LB medium (sodium chloride, 5g; yeast powder, 5g; peptone, 10g) and cultivate for about 48h; Centrifuge at 3000r / min for 20min, remove the supernatant, wash with 0.9% normal saline, repeat the centrifugation twice, then add 0.9% normal saline to prepare Shewanella Oneida suspension;
[0048] (3) Add 0.2 g of activated carbon to 12 mL of Shewanella Oneida bacteria suspension, mix and adsorb f...
Embodiment 2~4
[0055] Adopt the same method as Example 1 to prepare Shewanella Oneida immobilized pellets, but in the PVA-SA suspension of step (1), the mass fraction of polyvinyl alcohol and sodium alginate becomes:
Embodiment 2
[0056] Embodiment 2: polyvinyl alcohol 2%, sodium alginate 1%;
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