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Fish oil degumming and decolorizing method

A fish oil and degumming technology, applied in the direction of oil/fat refining, fat production, etc., can solve the problems of many side reactions, complex process, high energy consumption, etc., and achieve the effect of short processing time, simplified process and low energy consumption

Active Publication Date: 2019-01-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for synchronously degumming and degumming from crude fish oil by combining enzymes and multi-liquids, aiming at problems such as complex process, many side reactions, high energy consumption and long time in the current crude fish oil degumming and decolorization process. decolorization method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 8g of crude enzyme solution containing phospholipase Ultra (100U / mL), add 4g of trisodium citrate dihydrate, mix well, add 4g of polyethylene glycol 400 and 4g of tuna oil, put it into a stoppered Erlenmeyer flask and mix evenly , dubbed into a multi-liquid phase system; react under stirring at 1000rpm, and control the reaction at 37°C for 12h. After the reaction, set the speed of centrifugation at 5000rpm for 10min to divide into four phases. The upper liquid phase product is the fish oil phase, the second layer is the colloid-rich solid phase, and the third and fourth layers are respectively the alcohol-rich phase or the fish oil-rich phase. In the salt phase, enzymes are mainly concentrated in the third layer. Then, add 7.5 ml of sodium hydroxide solution with a concentration of 5 mol / L, then add an equal volume of n-hexane, and extract after standing, and the extracted upper phase is distilled to obtain refined fish oil. Its degumming and decolorization rate r...

Embodiment 2

[0029] Get 9g of crude enzyme solution (100U / mL) containing lipase PL20000L, add 3g of sodium sulfate, add 4g of polyethylene glycol 600 and 4g of tuna oil after mixing, put into a stoppered Erlenmeyer flask and mix evenly, and make multi Liquid phase system; react under stirring at 1000rpm, and control the reaction at 37°C for 1h. After the reaction, set the speed of centrifugation at 5000rpm for 10min to divide into four phases. The upper liquid phase product is the fish oil phase, the second layer is the colloid-rich solid phase, and the third and fourth layers are respectively the alcohol-rich phase or the fish oil-rich phase. In the salt phase, enzymes are mainly concentrated in the third layer. Then, add 7.5 ml of sodium hydroxide solution with a concentration of 5 mol / L, then add an equal volume of n-hexane, and extract after standing, and the extracted upper phase is distilled to obtain refined fish oil. Its degumming and decolorization rate reached 89.6% and 60% resp...

Embodiment 3

[0031] Take 8g of crude enzyme solution containing phospholipase Ultra (100U / mL), add 4g of trisodium citrate dihydrate, mix well, add 4g of [BMIM]BF4 and 4g of tuna oil, put into a stoppered Erlenmeyer flask and mix evenly, Prepare a multi-liquid phase system; react under stirring at 1000rpm, and control the reaction at 37°C for 12h. After the reaction, set the speed of centrifugation at 5000rpm for 10min to divide into four phases. The upper liquid phase product is the fish oil phase, the second layer is the colloid-rich solid phase, and the third and fourth layers are respectively the alcohol-rich phase or the fish oil-rich phase. In the salt phase, enzymes are mainly concentrated in the third layer. Then, add 7.5 ml of sodium hydroxide solution with a concentration of 5 mol / L, then add an equal volume of n-hexane, and extract after standing, and the extracted upper phase is distilled to obtain refined fish oil. The degumming and decolorizing rates reached 99.26% and 58.20...

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Abstract

The invention belongs to the technical field of biological engineering and food, and discloses a fish oil degumming and decolorizing method. The fish oil degumming and decolorizing method comprises the following steps: (1) preparing an enzyme solution with phospholipase or / and lipase concentration of 1 to 5000 U / mL, adding soluble salt, a hydrophilic solvent and fish oil into the enzyme solution,and preparing a three-liquid-phase system; (2) enabling the prepared three-liquid-phase system to react under a stirring condition, after the reaction, standing or centrifuging to form four layers, and collecting a fish oil phase on the topmost layer; (3) adding the fish oil phase into alkali liquor, then adding a hydrophobic organic solvent, standing, collecting an upper-phase, recycling the hydrophobic organic solvent, extracting, and collecting the supernatant, thus obtaining the refined fish oil. The invention provides the method for synchronously degumming and decolorizing the crude fishoil by combining the enzyme and multiple liquid phases, so that the process is simplified, the treatment time is short, the cost is reduced, and the method is economical and feasible.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, and relates to the separation and application technology of enzymes, in particular to a method for degumming and decolorizing crude fish oil by enzymatic pretreatment. Background technique [0002] In recent years, because fish oil is rich in polyunsaturated fatty acids, it has the effects of anti-cancer, preventing coronary heart disease, lowering blood pressure, and promoting brain development. Widespread attention outside. However, its raw material - crude fish oil has many impurities, high chroma, complex components and other factors, resulting in difficult decolorization and degumming, complex process, cumbersome steps and huge loss rate, which has become a technical problem that has plagued fish oil companies for many years. Therefore, , it is urgent to develop a simple and efficient refining technology. [0003] In recent decades, scientists have made a series of attempt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00
CPCC11B3/001C11B3/003C11B3/006
Inventor 李志刚杨博王永华陈华勇陆德林陈华
Owner SOUTH CHINA UNIV OF TECH
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