Diarrhea pathogen multi-gene detection system as well as kit and application thereof

Abstract

The invention relates to a diarrhea pathogen multi-gene detection system as well as a kit and application thereof. The detection system comprises 22 pairs of primers, wherein the 22 pairs of primers comprise 19 pairs of diarrhea pathogens, 2 pairs of human genome internal references and 1 pair of system quality control internal reference primers. The diarrhea pathogens are used for treating campylobacter jejuni, shigella, clostridium difficile, salmonella enteritidis, salmonella typhimurium, enterotoxin of escherichia coli, escherichia coli O157, vibrio, yersinia enterocolitica, human astrovirus, norovirus II, human enteric adenovirus, rotavirus and the like. According to the diarrhea pathogen multi-gene detection system and the kit thereof, steps including conventional culture and the like do not need to be adopted and synchronous detection and analysis of a plurality of types of the diarrhea pathogens can be directly carried out on an excrement sample in the same reaction system; the disadvantages of a conventional detection method that the flux is low, the consumed time is long, the detection rate is low and the like are made up; comprehensive, precision and low-cost pathogen diagnosis is provided for clinic at the first time and important reference is provided for individualized drug administration and precision medical treatment.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Infectious diarrhea is an intestinal infectious disease caused by a variety of pathogens, and is one of the most common digestive system infectious diseases worldwide. There are about 2 billion diarrheal diseases in the world every year, and about 800 million people suffer from diarrhea every year in my country. Infectious diarrhea has become the second largest infectious disease affecting human health after respiratory tract infection. [0003] Etiological diagnosis is of great significance to the prevention and treatment of infectious diarrhea. "World Organization of Gastroenterology Global Guidelines - Acute Diarrhea in Adults and Children: Global Perspectives (2012)" and "Expert Consensus on the Diagnosis and Treatment of Acute Infectious Diarrhea in Chinese Adults (2013)" cl...

AI Technical Summary

Problems solved by technology

However, the current routine detection methods have the disadvantages of low detection rate, long time-consuming, especially the inability to accurately identify multiple pathogens at the same time, and cannot provide timely, comprehensive and accurate pathogen diagnosis basis for clinical practice, leading to the widespread use of broad-spectrum Empirical treatment with antimicrobial drugs leads to problems such as low curative effect, failure to control diarrhea in time, high incidence of drug-resistant strains, and disturbance of digestive flora

[0009] In summary, current methods for the detection and diagnosis of diarrheal pathogens cannot meet the clinical needs, and the development of new technologies is urgently needed

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