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Preparation method of hemoglobin quality control product

A technology of hemoglobin and hemoglobin, which is applied in the preparation methods of peptides, hemoglobin/myoglobin, chemical instruments and methods, etc., can solve problems such as poor stability and easy loss of antigenic activity, and achieve good stability, low cost, and protection. active effect

Active Publication Date: 2017-10-20
SICHUAN ORIENTER BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for preparing a hemoglobin control product, aiming to solve the problem that the current hemoglobin is stored in liquid form, easily loses antigenic activity and has poor stability

Method used

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  • Preparation method of hemoglobin quality control product
  • Preparation method of hemoglobin quality control product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method for a hemoglobin control product, comprising the following steps in turn:

[0041] 1) Ingredients: Take 1-5ml of fresh whole blood from healthy people, add anticoagulant, and the anticoagulant is EDTAK2. Fresh whole blood from healthy people is negative for HIV, HCV, HBsAg and TP.

[0042] 2) Washing and centrifuging: adding 8 to 10 times the volume of physiological saline to the ingredients, which is 0.9% NaCl solution. Centrifuge at 1000 rpm for 10 minutes and remove the supernatant. The washing and centrifuging process was repeated 8-10 times.

[0043] 3) Obtain erythrocyte fluid: after washing and centrifugation, add physiological saline to restore the volume of fresh blood from healthy people in step 1), and obtain erythrocyte fluid.

[0044] 4) Extraction of hemoglobin: Add polyethylene glycol octylphenyl ether with a volume ratio of 0.5-2% to the red blood cell fluid and shake and mix on a vortex mixer for 10-15 minutes to rupture the red ...

Embodiment 2

[0070] A preparation method for a hemoglobin control product, comprising the following steps in turn:

[0071] 1) Ingredients: Take 1-5ml of fresh whole blood from healthy people, add anticoagulant, and the anticoagulant is EDTAK2. Fresh whole blood from healthy people is negative for HIV, HCV, HBsAg and TP.

[0072] 2) Washing and centrifugation: add 8 times the volume of the batch of physiological saline to the ingredients, and the physiological saline is 0.8%-1% NaCl solution. Centrifuge at 800 rpm for 8 minutes and remove the supernatant. The washing and centrifugation process was repeated 8 times.

[0073] 3) Obtain erythrocyte fluid: after washing and centrifugation, add physiological saline to restore the volume of fresh blood from healthy people in step 1), and obtain erythrocyte fluid.

[0074] 4) Extraction of hemoglobin: Add 0.5% polyethylene glycol octylphenyl ether in a volume ratio to the erythrocyte liquid and shake and mix on a vortex mixer for 10 minutes to...

Embodiment 3

[0094] A preparation method for a hemoglobin control product, comprising the following steps in turn:

[0095] 1) Ingredients: take 5ml of fresh whole blood from a healthy person, add anticoagulant, and the anticoagulant is EDTAK2. Fresh whole blood from healthy people is negative for HIV, HCV, HBsAg and TP.

[0096] 2) Washing and centrifugation: adding 10 times the volume of the batching of physiological saline to the batching, the physiological saline is 1% NaCl solution. Centrifuge at 1100 rpm for 11 minutes and remove the supernatant. The washing and centrifugation process was repeated 10 times.

[0097] 3) Obtain erythrocyte fluid: after washing and centrifugation, add physiological saline to restore the volume of fresh blood from healthy people in step 1), and obtain erythrocyte fluid.

[0098] 4) Extract hemoglobin: Add polyethylene glycol octylphenyl ether with a volume ratio of 2% to the erythrocyte liquid and shake and mix on a vortex mixer for 15 minutes to rupt...

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Abstract

The invention discloses a preparation method of a hemoglobin quality control product. The preparation method comprises following steps: 1, material preparation; 2, washing and centrifugation; 3, obtaining of an erythrocyte solution; 4, extraction of hemoglobin; 5, dialysis; and 6, sub-packaging of freeze-dried powder. According to the preparation method, hemoglobin obtained via dialysis is added into a diluent containing a freeze-drying protective agent and a filling agent for dilution to a set quality control product concentration; after sub-packaging, low temperature pre-freezing and freeze-drying are carried out so as to obtain freeze-dried powder. In the preparation process, the activity of hemoglobin is protected effectively; the form of freeze-dried powder is adopted for storage, so that the stability is high, and the performance of the reagent can be monitored in clinical applications effectively. The preparation method is low in cost; operation is simple; large scale production can be realized; and long term storage can be realized.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of a hemoglobin control product. Background technique [0002] Feces occult blood: also known as feces occult blood, refers to a small amount of bleeding in the digestive tract, red blood cells are digested and destroyed, no abnormal changes in the appearance of feces, and bleeding that cannot be confirmed by naked eyes and microscopes. [0003] Since fecal occult blood cannot be directly detected by the naked eye, the vast majority of patients with early digestive tract malignancies cannot be detected and treated early, thus delaying the best time for treatment, lurking in our body like a hidden killer. [0004] Red blood cells contain hemoglobin, which makes blood red. If a red blood cell ruptures, hemoglobin is released. A small amount of gastrointestinal bleeding does not cause changes in stool color, red blood cells are digested and destroyed, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805C07K1/14
CPCC07K14/805
Inventor 陈功台
Owner SICHUAN ORIENTER BIOLOGICAL TECH
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