Method for extracting raspberry polysaccharides component having function for removing free radical

An extraction method, the technology of raspberry, is applied in the field of raspberry polysaccharide component extraction, which can solve the problems of low yield and purity of polysaccharide components, increase the cost of preparation, etc., and achieve the effect of improving the purity

Inactive Publication Date: 2017-10-20
安徽博格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, studies have shown that the polysaccharide components of raspberry have a strong ability to scavenge oxygen free radicals, but the yield and purity of the polysaccharide components extracted from raspberry by conventional extraction methods are low, which increases its preparation cost

Method used

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  • Method for extracting raspberry polysaccharides component having function for removing free radical
  • Method for extracting raspberry polysaccharides component having function for removing free radical
  • Method for extracting raspberry polysaccharides component having function for removing free radical

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Cleaning: Soak the raspberries in clean water for 10 minutes, and wash them twice with water 3 times the dry weight of the raspberries, stirring for 5 minutes each time;

[0027] (2) Drying and crushing: dry the cleaned raspberries with hot air at 45-50° C., then pulverize them into powder with a pulverizer, and pass through a 100-mesh sieve to obtain raspberry powder;

[0028] (3) Extraction: the above-mentioned raspberry powder was extracted three times with reflux water, each time using 5 times of raspberry dry weight distilled water to extract for 1.5h, and adding 0.5% of raspberry dry weight extract enhancer, combined filtrate, filtrate Concentrate under reduced pressure until the specific gravity reaches 1.11-1.15, add 95% ethanol after natural cooling to room temperature, so that the ethanol concentration in the resulting mixture reaches 80%, stir thoroughly, let stand for 5 hours, and filter again to obtain the crude polysaccharide;

[0029] (4) Dissolving: ...

Embodiment 2

[0037] (1) Cleaning: Soak the raspberries in clean water for 10 minutes, and wash them twice with water 3 times the dry weight of the raspberries, stirring for 5 minutes each time;

[0038] (2) Drying and crushing: dry the cleaned raspberries with hot air at 45-50° C., then pulverize them into powder with a pulverizer, and pass through a 100-mesh sieve to obtain raspberry powder;

[0039](3) Extraction: the above-mentioned raspberry powder was extracted three times with reflux water, each time using 5 times of raspberry dry weight distilled water to extract for 1.5h, and adding 0.5% of raspberry dry weight extract enhancer, combined filtrate, filtrate Concentrate under reduced pressure until the specific gravity reaches 1.11-1.15, add 95% ethanol after natural cooling to room temperature, so that the ethanol concentration in the resulting mixture reaches 80%, stir thoroughly, let stand for 5 hours, and filter again to obtain the crude polysaccharide;

[0040] (4) Dissolving: t...

Embodiment 3

[0090] Utilize respectively embodiment 1, embodiment 2, comparative example 1, comparative example 2, comparative example 3 and the method described in comparative example 4 to carry out polysaccharide composition to the same batch of raspberry, measure its polysaccharide composition The extraction yield and purity are shown in Table 1.

[0091] Table 1 polysaccharide composition extraction yield and purity of the present invention

[0092]

[0093]

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PUM

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Abstract

The invention discloses a method for extracting a raspberry polysaccharides component having function for removing free radical, which relates to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: 1) cleaning; 2) drying and crushing; 3) extracting; 4) dissolving 5) performing primary purifying; 6) performing secondary purifying; and 7) drying. The method uses an alcohol extracting-water precipitating method for increasing the extraction rate of the polysaccharides component and performing crude separation on the impurity, at the same time, the addition of an extraction-promotion agent can obviously increase the extraction rate of the polysaccharides component, so that the extraction rate can reach 6.52%; a polyamide column and a DEAE-cellulose anion column are used for two-time purification, the purity of the polysaccharides component can be effectively increased, and the purity can reach more than 80%.

Description

Technical field: [0001] The invention relates to the technical field of extracting traditional Chinese medicines, in particular to a method for extracting raspberry polysaccharide components with the function of scavenging free radicals. Background technique: [0002] Raspberry, also known as raspberry, is a berry plant of the genus Rubus in the Rosaceae family. Raspberry is a dual-purpose fruit for medicine and food. It is rich in essential nutrients such as amino acids, mineral elements, organic acids, and vitamin E. It also contains functional substances such as flavonoids, polysaccharides, and crude triterpenes. Therefore, raspberries have anti-aging, It can regulate the reproductive system, promote cellular immune function and lose weight, and has excellent antibacterial and immune-enhancing effects. [0003] At present, studies have shown that the polysaccharide components of raspberry have a strong ability to scavenge oxygen free radicals, but the yield and purity of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张新宇
Owner 安徽博格生物科技有限公司
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