Streptomyces roseospora l31 with high daptomycin production and its construction method

A technology of Streptomyces roseospora and daptomycin, which is applied in the field of Streptomyces roseospora L31 with high yield of daptomycin and its construction field, which can solve the problems of unsatisfactory industrial production and clinical application, low fermentation unit of daptomycin, etc. problems, to achieve the effect of increasing supply, increasing production and speeding up

Active Publication Date: 2021-03-26
ZHEJIANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the fermentation unit of daptomycin is low, which cannot meet the needs of industrial production and clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptomyces roseospora l31 with high daptomycin production and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The culture medium used in this embodiment:

[0015] 1. R5 solid medium: 10.3% sucrose, 1% glucose, 0.5% yeast extract, 0.01% casein hydrolyzate, K 2 SO 4 0.025%, MgCl 2 ·6H 2 O 1.012%, trace element solution 0.2%, trimethylolmethylaminoethanesulfonic acid 0.573%, proline 0.03%, agar powder 2.2%. After sterilization, add 5% KH to every 1L medium 2 PO 4 1mL, 5MCaCl 2 4mL, 700μL of 10M NaOH, the rest is water, the stated percentages are mass percentages, and the pH is natural

[0016] Trace element solution: CuCl 2 2H 2 O 0.1g, Na 2 B 4 o 7 10H 2 O 0.1g, MnCl 2 4H 2 O 0.1g, ZnCl 2 0.4g, (NH4)Mo 7 o 24 4H 2 O 0.1g, FeCl 3 ·6H 2 O 2g, add water to make up to 1L

[0017] 2. TSB medium: 2% of tryptone soybean broth, 5% of polyethylene glycol (PEG600), and the rest is water, the percentages are all by mass, and the pH is natural.

[0018] 3. YEME fermentation medium: 0.3% of yeast extract, 0.5% of peptone, 0.3% of malt extract, 4% of glucose, and the r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a Streptomyces roseosporus (Streptomyces roseosporus) L31 with high yield of daptomycin, which fuses two enzymes (DptE and DptF) in the initial reaction of the synthesis pathway of daptomycin, and directional transforms tryptophan The production of daptomycin in this strain was increased by 140% compared with the original strain (WT).

Description

technical field [0001] The invention relates to the field of microbial pharmacy, in particular to a high-daptomycin-producing Streptomyces roseospora L31 and a construction method thereof. Background technique [0002] Due to the widespread use of antibiotics in clinical practice, the drug resistance of pathogenic bacteria has become more and more serious. Humans urgently need new antibiotics to combat the emergence of drug-resistant bacteria. Daptomycin is a cyclolipopeptide antibiotic extracted from the fermentation broth of Streptomyces roseosporus, which has strong antibacterial activity against Gram-positive bacteria and is considered to be the best substitute for vancomycin . Daptomycin binds to the cell membrane of pathogenic bacteria, causing ion outflow and causing their death. Because of its unique cyclolipopeptide structure and mechanism of action, and its lack of cross-resistance with other antibiotics, daptomycin has broad application prospects. [0003] In S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12P21/04C12R1/465
CPCC07K7/08C12P21/02C12N1/205C12R2001/465
Inventor 李永泉毛旭明袁鹏辉
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap