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Production method of epsilon-polylysine

A production method, polylysine technology, applied in the field of microbial fermentation, can solve problems such as increased cost, long fermentation cycle, and impact on industrial production efficiency, and achieve the effects of improving efficiency, improving filtration efficiency, and shortening production time

Active Publication Date: 2017-10-20
BEIJING DATSING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing industrial fermentation process has been improved for many years, the fermentation cycle is still very long, which affects the efficiency of industrial production and increases the cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of production method of ε-polylysine

[0029] The present embodiment provides a kind of production method of ε-polylysine, and concrete operation steps are:

[0030] 1) Preparation of ε-polylysine fermentation broth:

[0031] A. Seed culture: first empty the seed tank, and remove the seed medium (NH 4 ) 2 SO 4 , yeast paste, Na 2 HPO 4 12H 2 O, MgSO 4 ·7H 2 Add O into the seed tank, then sterilize the seed tank, add glucose after cooling down, adjust the temperature at 25-30°C, use 1mol / L ammonia water or NaOH to control the pH value at 5.5-6.0, the ventilation rate is 4L / min, add strains, cultivated for 24 hours to obtain seed culture solution;

[0032] The formula of seed medium is as follows: glucose 15g / L, (NH 4 ) 2 SO 4 5g / L, yeast extract 5g / L, Na 2 HPO 4 12H 2 O 1g / L, MgSO 4 ·7H 2 O 0.5g / L.

[0033] B. Fermentation culture: first empty the fermenter tank, and remove the fermentation medium (NH 4 ) 2 SO 4 , Na 2 HPO 4 12...

Embodiment 2

[0036] Embodiment 2 A kind of production method of ε-polylysine

[0037] This embodiment provides a production method of ε-polylysine, which is the same as the method described in Example 1, the difference is that diatomite in Example 1 is replaced by FeSO 4 , control the iron ion concentration in the fermentation medium to be the same as the iron ion concentration in Example 1.

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Abstract

The invention relates to a production method of epsilon-polylysine. Kitasatospora is used as a strain and undergoes seed culture and fermentation cuture, wherein a fermentation culture medium contains an iron source. The production method of the epsilon-polylysine can shorten the fermentation cycle, improve the industrialization efficiency, increase the yield and reduce the cost.

Description

technical field [0001] The invention relates to a production method of ε-polylysine, which belongs to the technical field of microbial fermentation. Background technique [0002] ε-polylysine is a polypeptide composed of 25 to 30 polylysine monomers, an essential amino acid for human body. Polylysine is a peptide bond formed by the dehydration condensation of the α-hydroxyl of a lysine monomer and the ε-amino group of a lysine monomer. ε-polylysine has good thermal stability, and ε-polylysine after high temperature treatment still has antibacterial properties; it has a wide antibacterial spectrum and can inhibit the growth of Gram-positive bacteria, Gram-negative bacteria and some pathogens Propagation; easily soluble in water; the decomposition product is lysine, which is one of the essential amino acids for the human body and has good safety. [0003] The industrial production of ε-polylysine adopts fermentation method. The ε-polylysine fermentation broth is obtained th...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12R1/01
CPCC12P13/02
Inventor 李俊昊张玉兰李湘杰李次会
Owner BEIJING DATSING BIO TECH