OsSBR1 gene controlling rice sheath blight resistance and application of RNA interference fragment of OsSBR1 gene

Abstract

The invention relates to an application of an OsSBR1 gene and an RNA interference fragment thereof for controlling rice sheath blight resistance. The OsSBR1 gene has disease resistance to rice sheath blight fungus under the condition of inhibiting expression in rice. The invention utilizes RNAi transgenic technology to obtain the OsSBR1 sheath blight resistant rice gene, which can be applied to the creation of sheath blight resistant transgenic rice and the cultivation of sheath blight resistant rice varieties; and the untransformed recipient rice variety "Taijing 394" In comparison, the sheath blight resistance of OsSBR1-RNAi transgenic plants is significantly improved; the present invention also confirms that the expression of OsSBR1 gene is suppressed, and rice disease resistance-related genes OsPR1, OsPR1b, PBZ1, E2F-related gene, PDR and PR3 are overexpressed, The resistance of the transgenic plants to sheath blight is significantly improved, which is a new discovery about the molecular mechanism of rice sheath blight resistance.

Description

technical field [0001] The invention relates to the field of plant genetic engineering and crop breeding, in particular to the application of OsSBR1 gene and its RNA interference fragment for controlling rice sheath blight resistance. Background technique [0002] Rice sheath blight is one of the three major diseases of rice in the world. The pathogen is Rhizoctonia solani, a soil-borne saprophytic fungus with a wide range of hosts. Sheath blight infects field rice from the tillering stage to the heading stage, starting from the leaf sheath near the water surface, and can also damage the leaves, stems and even rice ears. Infected rice is prone to lodging, no heading, increased number of shriveled and empty grains or white ears. The light ones will reduce production by about 10%, and the serious ones will reduce production by more than 50%. At present, the prevention and control of rice sheath blight mainly relies on costly and polluting chemical control and low-efficiency...

AI Technical Summary

Problems solved by technology

[0005] To sum up, although the control of rice sheath blight is very important for rice production, the main resistance gene of the disease has not been found in the current production practice, and no rice varieties with high resistance to sheath blight have been bred.

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