OsSBR1 gene controlling rice sheath blight resistance and application of RNA interference fragment of OsSBR1 gene

A technology of RNA interference and rice sheath blight bacteria, which is applied in the fields of plant genetic engineering and crop breeding, can solve the problems of not finding the main disease-resistant gene and not breeding rice varieties with high resistance to sheath blight

Inactive Publication Date: 2017-10-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

[0005] To sum up, although the control of rice sheath blight is very important for rice production, the main resistance gene of the disease has not been found in the current production practice, and no rice varieties with high resistance to sheath blight have been bred.

Method used

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  • OsSBR1 gene controlling rice sheath blight resistance and application of RNA interference fragment of OsSBR1 gene
  • OsSBR1 gene controlling rice sheath blight resistance and application of RNA interference fragment of OsSBR1 gene
  • OsSBR1 gene controlling rice sheath blight resistance and application of RNA interference fragment of OsSBR1 gene

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Embodiment

[0020]1. Gene chip screening of the sheath blight-induced differentially expressed gene OsSBR1: Lemont is an internationally recognized sheath blight-susceptible rice variety, and YSBR1 was selected as the "Three Crop Sheath Blight Bacteria" by the National Public Welfare Industry (Agriculture) Research Project in 2007 Relatively disease-resistant rice varieties recognized by the Research Collaborative Group of Species and Host Resistance Identification Technology. The strong virulence Rhizoctonia solani YN-7 strain was inoculated into YSBR1 (moderately resistant) / Lemont (susceptible) adult rice plants, and the leaf sheath tissues were collected at 0h, 6h, 10h, and 20h after inoculation to extract total RNA. Each total RNA sample was subjected to gene chip differential expression screening containing 15,000 genes focusing on rice disease resistance pathways, and the microarray screening was handed over to Shanghai Bohao Company. The results of gene chip data analysis showed th...

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Abstract

The invention relates to an application of an OsSBR1 gene and an RNA interference fragment thereof for controlling rice sheath blight resistance. The OsSBR1 gene has disease resistance to rice sheath blight fungus under the condition of inhibiting expression in rice. The invention utilizes RNAi transgenic technology to obtain the OsSBR1 sheath blight resistant rice gene, which can be applied to the creation of sheath blight resistant transgenic rice and the cultivation of sheath blight resistant rice varieties; and the untransformed recipient rice variety "Taijing 394" In comparison, the sheath blight resistance of OsSBR1-RNAi transgenic plants is significantly improved; the present invention also confirms that the expression of OsSBR1 gene is suppressed, and rice disease resistance-related genes OsPR1, OsPR1b, PBZ1, E2F-related gene, PDR and PR3 are overexpressed, The resistance of the transgenic plants to sheath blight is significantly improved, which is a new discovery about the molecular mechanism of rice sheath blight resistance.

Description

technical field [0001] The invention relates to the field of plant genetic engineering and crop breeding, in particular to the application of OsSBR1 gene and its RNA interference fragment for controlling rice sheath blight resistance. Background technique [0002] Rice sheath blight is one of the three major diseases of rice in the world. The pathogen is Rhizoctonia solani, a soil-borne saprophytic fungus with a wide range of hosts. Sheath blight infects field rice from the tillering stage to the heading stage, starting from the leaf sheath near the water surface, and can also damage the leaves, stems and even rice ears. Infected rice is prone to lodging, no heading, increased number of shriveled and empty grains or white ears. The light ones will reduce production by about 10%, and the serious ones will reduce production by more than 50%. At present, the prevention and control of rice sheath blight mainly relies on costly and polluting chemical control and low-efficiency...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N15/113C12N15/8218C12N15/8282C12N2310/14
Inventor 瞿绍洪袁正杰毕冬玲邹小维高晓庆徐国娟马晨燕何海燕张玉杨海河曲海艳赵建华
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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