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Method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria

A technology of plants and degrading bacteria, applied in botany equipment and methods, preservation of human or animal bodies, chemicals for biological control, etc., can solve secondary soil pollution, PAHs removal and degradation technology is rarely involved, The repair process is not easy to control and other problems, to achieve the effect of easy operation and high removal rate

Active Publication Date: 2017-11-03
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, physical or chemical methods often cause secondary pollution to the soil, the restoration process is not easy to control, and large-scale applications still need to solve a large number of problems
Biodegradation of PAHs is an efficient and environmentally friendly way, but current research mainly focuses on the removal and degradation of PAHs in soil, and the removal and degradation of PAHs in plants is rarely involved

Method used

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  • Method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria
  • Method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria
  • Method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The Shanghai Qing seeds were inoculated with compound functional bacteria to reduce the PAHs content in the body.

[0027] Preparation of microbial composite functional bacteria agent:

[0028] The composite functional bacteria is composed of 8 strains of PAHs degrading bacteria with different degradation spectrums, namely: Sphingobium sp. RS1, RS2), Mycobacterium sp. Pyr9, 033), (Diaphorobacter sp. Phe15), Massilia sp.Pn2, Paenibacillus sp.Phe3, Pseudomonas sp.Ph6;

[0029] Cultivate each strain separately, and then adjust the OD of each strain 600nm Mixing to make a microbial compound functional bacterial agent specifically includes the following steps:

[0030] (1) Preparation of bacterial suspension: 8 strains of PAHs-degrading bacteria were activated on LB solid plates, and each single colony was picked from the activated plate with an inoculation loop and inoculated in sterile LB liquid medium, placed at 30°C, 150r Centrifuge at 8000r / min at 4°C for 5 minutes a...

Embodiment 2

[0035] Inoculate the leaves of Shanghai green leaves with compound functional bacteria to reduce the PAHs content in the body.

[0036] Preparation of microbial composite functional bacteria agent:

[0037] The composite functional bacteria is composed of 8 strains of PAHs degrading bacteria with different degradation spectrums, namely: Sphingobium sp. RS1, RS2), Mycobacterium sp. Pyr9, 033), (Diaphorobacter sp. Phe15), Massilia sp.Pn2, Paenibacillus sp.Phe3, Pseudomonas sp.Ph6;

[0038] Cultivate each strain separately, and then adjust the OD of each strain 600nm Mixing to make a microbial compound functional bacterial agent specifically includes the following steps:

[0039] (1) Preparation of bacterial suspension: 8 strains of PAHs-degrading bacteria were activated on LB solid plates, and each single colony was picked from the activated plate with an inoculation loop and inoculated in sterile LB liquid medium, placed at 30°C, 150r Centrifuge at 8000r / min at 4°C for 5 min...

Embodiment 3

[0044] The Shanghai Qing seeds were inoculated with compound functional bacteria to reduce the PAHs content in the body.

[0045] Preparation of microbial composite functional bacteria agent:

[0046] The composite functional bacteria is composed of 8 strains of PAHs degrading bacteria with different degradation spectrums, namely: Sphingobium sp. RS1, RS2), Mycobacterium sp. Pyr9, 033), (Diaphorobacter sp. Phe15), Massilia sp.Pn2, Paenibacillus sp.Phe3, Pseudomonas sp.Ph6;

[0047] Cultivate each strain separately, and then adjust the OD of each strain 600nm Mixing to make a microbial compound functional bacterial agent specifically includes the following steps:

[0048] (1) Preparation of bacterial suspension: 8 strains of PAHs-degrading bacteria were activated on LB solid plates, and each single colony was picked from the activated plate with an inoculation loop and inoculated in sterile LB liquid medium, placed at 30°C, 150r Centrifuge at 8000r / min at 4°C for 5 minutes a...

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Abstract

The invention discloses a method for simultaneously removing USEPA PAHs in plant bodies by utilizing compound PAHs degrading bacteria. Multiple functional bacteria are prepared into a microbial compound functional fungicide, a plant absorption promoter, cytokinin 6-BA or KT, auxin 2,4-D and cysteine are added to prepare a degradation agent, then the degradation agent is inoculated in the plant bodies, and the PAHs content in the plant body is reduced; the compound fungicide is prepared from 8 PAHs degrading bacteria different in degradation spectrum, wherein the degrading bacteria are Sphingobiumsp sp. RS1 and RS2, mycobacterium sp. Pyr9, 033, Diaphorobacter sp. Phe15 and Massilia sp. Pn2, Paenibacillus sp. Phe3, Pseudomonas sp. Ph6 respectively. The acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a] anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h] anthracene, benzo[ghi]indene and [1,2,3-cd] pyrene in each plant body in a contaminated area can be effectively removed and degraded, the removal rate of PAHs is high, and the method has the advantages of high efficiency, environmental protection and easy operation.

Description

technical field [0001] The invention relates to the technical field of environmental engineering microorganisms, in particular to a method for simultaneously removing USEPA PAHs in plants by using composite PAHs degrading bacteria. Background technique [0002] PAHs are persistent, high-risk organic pollutants. It has been found that there are hundreds of known PAHs, among which 16 PAHs are listed as priority control PAHs by the U.S. Environmental Protection Agency because of the "three-induced" effect of teratogenicity and mutagenicity. Soil is one of the important natural resources for human survival. Sewage irrigation, atmospheric dry and wet deposition, etc. have caused soil pollution in my country and the world to become increasingly serious. In 2014, the National Soil Pollution Survey Bulletin released by the Ministry of Environmental Protection and the Ministry of Land and Resources showed that the national soil PAHs point exceeding the standard rate was 1.4%. Afte...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01G7/06A01N63/00A01N43/90A01N43/38A01N37/44A01P21/00
CPCA01C1/00A01G7/06A01N37/44A01N43/38A01N43/90A01N63/00A01N2300/00
Inventor 高彦征王建刘娟凌婉婷
Owner NANJING AGRICULTURAL UNIVERSITY
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