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Method for extracting alpha-amylase and alpha-glucosidase inhibitor from bangia fusco-purpurea

A technology of glucosidase and amylase, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, metabolic diseases, etc., can solve the problems of unseen extraction of α-amylase and α-glucosidase inhibitors, and achieve Effect of suppressing blood sugar rise

Inactive Publication Date: 2017-11-03
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on how to efficiently utilize red hair algae resources, and there are no reports or patents on extracting α-amylase and α-glucosidase inhibitors from red hair algae

Method used

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  • Method for extracting alpha-amylase and alpha-glucosidase inhibitor from bangia fusco-purpurea
  • Method for extracting alpha-amylase and alpha-glucosidase inhibitor from bangia fusco-purpurea
  • Method for extracting alpha-amylase and alpha-glucosidase inhibitor from bangia fusco-purpurea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the extraction of inhibitor

[0022] The fresh red hair algae was purchased from Putian, Fujian. The extraction is carried out by water extraction and alcohol precipitation method, and the specific operation is as follows: clean the fresh red hair algae, dry them in an oven at 50°C, and then pulverize them with a pulverizer. Add 3 to 5 times the volume of methanol to the dried algae powder, stir continuously, and wash repeatedly three times to ensure sufficient decolorization, and soak overnight. After soaking, centrifuge at 4000r / min for 15 minutes, take out the precipitate, add distilled water and heat it in a 90°C water bath for 2 hours, centrifuge at 4000r / min for 20 minutes, take the supernatant, repeat the hot water extraction of the precipitate for 2 hours and then centrifuge, and combine the supernatants of the two centrifuges Liquid, concentrated by evaporation, the working temperature is 50°C, and the extract is concentrated to 1 / 10 of the origi...

Embodiment 2

[0024] Example 2: Gel Filtration Purification of Polysaccharides

[0025] The crude polysaccharide was purified by Sephadex G75 gel column (C16 / 100), and the specific operation was as follows: the concentration of the sugar solution was 5 mg / mL, the sample volume was 3 mL, and eluted with 0.1 M NaCl solution. Use an automatic collector to receive, receive 50 tubes, each tube is 5mL. Use the phenol sulfuric acid method to track and detect the elution at 490nm. Take the tube number as the abscissa and the absorbance value as the ordinate to make an elution diagram. The eluted peaks were combined, desalted by dialysis, and freeze-dried to obtain a preliminary purified polysaccharide F. The result is as figure 1 As shown, the elution curve of the crude polysaccharide of red hair algae obtained a single symmetrical peak. Since the crude polysaccharide was deproteinized before purification, a single component was eluted, which was named F, indicating that in the F component It is...

Embodiment 3

[0026] Example 3: Determination of activity of red hair algae α-amylase inhibitor and comparison with acarbose amylase inhibitory activity

[0027] Use 3,5-dinitrosalicylic acid method to measure, take 100 μl red hair algae polysaccharide solution with different dilution times, add 100 μl α-amylase solution respectively, after mixing for 10 minutes, add 200 μl soluble starch solution (1%) to start For the reaction, the reaction mixture was placed in a constant temperature water bath at 37°C for 5 minutes, and then 1 mL of DNS reagent was added to terminate the reaction. The reaction solution was placed in a boiling water bath for 5 minutes. After taking it out, it was cooled rapidly in an ice bath, and diluted with 3 mL of distilled water. Measure the absorbance at 540nm, replace the enzyme solution with 0.5mL phosphate buffer (pH 6.8, 0.05M) as a blank control, and use acarbose as a positive control, IC 50 The value is the concentration of the sugar solution when the enzyme a...

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Abstract

The invention discloses a method for extracting an alpha-amylase and alpha-glucosidase inhibitor from bangia fusco-purpurea,which comprises the following steps of 1, extraction: cleaning, drying and smashing fresh bangia fusco-purpurea to obtain bangia fusco-purpurea powder, adding distilled water, extracting and concentrating to obtain a concentrated solution; 2, alcohol precipitation: dropwise adding ethyl alcohol, centrifuging, taking sediments, performing freeze drying to prepare crude polysaccharide; 3, gel chromatography: dissolving the crude polysaccharide into distilled water, sample loading, eluting, tracing and checking, collecting eluting peaks, performing dialysis desalting, andperformingfreeze drying to obtain purified polysaccharide, so that the alpha-amylase and alpha-glucosidase inhibitor is prepared. The method provided by the invention has the advantages that the preparation technology is simple, the cost is low, and the extracted alpha-amylase and alpha-glucosidase inhibitor has the activity for inhibiting blood glucose elevation.

Description

technical field [0001] The invention relates to the technical field of active polysaccharides, in particular to a method for extracting alpha-amylase and alpha-glucosidase inhibitors from red hair algae. Background technique [0002] In recent years, high blood sugar has increasingly become one of the important factors that plague people's health. Long-term high blood sugar will cause lesions in various tissues and organs of the body, leading to acute and chronic complications, such as decreased resistance, impaired renal function, cardiovascular and cerebrovascular diseases, diabetes, etc. In recent years, many researchers at home and abroad have begun to explore natural active substances that can inhibit the rise of blood sugar. Most studies have shown that α-amylase and α-glucosidase are closely related to blood sugar in the body. α-amylase and α-glucosidase play a key role in the digestion and absorption of starch and other major carbohydrates in food, and inhibiting th...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCA61K31/715C08B37/00C08B37/0003
Inventor 姜泽东余刚倪辉宋田源伍菱杨远帆李利君朱艳冰蔡慧农
Owner JIMEI UNIV
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