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Tissue engineered skin containing living cells and its preparation method

A technology of tissue engineering skin and living cells, which is applied in the field of tissue engineering skin containing living cells and its preparation, can solve the problems of poor wound dressing effect, poor isolation ability, long degradation time, etc. Effect of improving mechanical strength, toughness and elasticity

Active Publication Date: 2020-03-10
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the defects of high cost, short shelf life and poor isolation ability of tissue engineered skin containing living cells in the prior art
However, the degradable polymer material protective layer used in this invention takes a long time to degrade in the human body, which may easily cause poor wound dressing effect

Method used

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  • Tissue engineered skin containing living cells and its preparation method
  • Tissue engineered skin containing living cells and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 culture medium kind and composition

[0041] A culture medium for epidermal cells is: DMEM / F12 is used as the base medium, and the additives and dosage are: bovine pituitary extract 50 μg / mL, hydrocortisone 5 ng / mL, cholera toxin 1×10 -10 mol / L, insulin 0.05ng / mL, adenine 1.8×10 -4 mol / L, penicillin 100IU / mL, transferrin 10μg / mL, glutamic acid 5μg / mL, epidermal growth factor 10μg / mL, ethanolamine 1×10 -5 mol / L;

[0042] A fibroblast culture medium is: DMEM / F12 is used as the base medium, and the additives and dosage are: bovine pituitary extract 50 μg / mL, insulin 5 ng / mL, hydrocortisone 300 μg / ml, adenine 15 μg / mL , vitamin C 120μg / mL, basic fibroblast growth factor 8μg / mL, transferrin 10μg / mL;

[0043] A keratinocyte induction medium is: DMEM / F12 is used as the base medium, and the additives and dosage are: 50 μg / mL of bovine pituitary gland extract, 100 IU / mL of penicillin, 100 μg / mL of streptomycin, and 20 ng of epidermal growth factor / mL, insulin 1...

Embodiment 2

[0048] Embodiment 2 A kind of tissue engineered skin containing living cells

[0049] Described tissue-engineered skin containing living cells comprises epidermis, artificial cortex and dermis, and described artificial cortex comprises keratinocytes, fibroblasts and biological scaffold material, wherein the density ratio of keratinocytes and fibroblasts is 0.15:1; the biological scaffold material is composed of gelatin, chitosan, glutamine peptide and hyaluronic acid in a mass ratio of 6.5:1.75:0.75:1, and its preparation method is: under the condition of 50°C, gelatin is prepared The mass fraction is 10% gelatin aqueous solution, the chitosan is dissolved in the acetic acid solution of 2% mass fraction to be configured into the acetic acid solution that the mass fraction is 2% chitosan, two kinds of solutions are mixed, and add glutamine peptide and Hyaluronic acid, stir evenly, let it stand for 30 hours, then cast the obtained mixed solution on a horizontal polyvinyl chlorid...

Embodiment 3

[0063] Embodiment 3 A kind of tissue engineered skin containing living cells

[0064] The difference from Example 2 is that the bioscaffold material is composed of gelatin, chitosan, glutamine peptide and hyaluronic acid in a mass ratio of 6:2:1:1, and the others are the same as in Example 2.

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PUM

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Abstract

The invention belongs to the technical field of tissue engineering and particularly relates to tissue engineering skin containing living cells and a preparation method of the tissue engineering skin. The tissue engineering skin comprises an epidermis layer, an imitated corium layer and a corium layer, wherein the imitated corium layer contains keratinocytes, fibroblasts and a bio-scaffold material, and the density ratio of the keratinocytes to the fibroblasts is (0.1-0.2) to 1; and the bio-scaffold material contains gelatin, chitosan, glutamine and hyaluronic acid in a mass ratio of (6-7) to (1.5-2) to (0.5-1) to 1. The tissue engineering skin prepared by virtue of the preparation method has good biocompatibility, plasticity and mechanical strength and can be used for repairing the surface of a wound with a good effect.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a living cell-containing tissue engineering skin and a preparation method thereof. Background technique [0002] Tissue engineering (tissue engineering) is a combination of cell biology, engineering and materials science to construct tissue or organ substitutes in vitro or in vivo for the purpose of repairing, maintaining or improving the shape and function of the body. Subject. Among them, tissue engineering skin is to use the principle of tissue engineering to construct artificial skin substitutes for skin transplantation, which can effectively solve the problem of skin defect repair and insufficient skin donor in clinical practice. [0003] Skin tissue engineering is the earliest and most mature field in tissue engineering. The first tissue engineering product approved by the FDA is tissue engineered skin. Clinically, skin ulcers include diabetic ulcer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/22A61L27/20A61L27/36A61L27/50A61L27/54A61L27/58A61L27/60
CPCA61L27/20A61L27/222A61L27/227A61L27/3604A61L27/3804A61L27/3834A61L27/50A61L27/54A61L27/58A61L27/60A61L2300/404A61L2300/412A61L2300/414C08L5/08C08L89/00
Inventor 刘少辉车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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