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Tissue engineering scaffold containing natural high-molecular microspheres/nanospheres and preparation method of tissue engineering scaffold

A tissue engineering scaffold and natural polymer technology, which is used in tissue regeneration, medical science, prosthesis, etc. Effect

Inactive Publication Date: 2017-11-07
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, to provide a tissue engineering scaffold containing natural polymer microspheres / nanospheres and a preparation method thereof, to solve the problem of uniformity of formed composite materials when LDM prepares tissue engineering scaffolds in the prior art. Technical issues such as sex and preparation process solvent constraints

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  • Tissue engineering scaffold containing natural high-molecular microspheres/nanospheres and preparation method of tissue engineering scaffold
  • Tissue engineering scaffold containing natural high-molecular microspheres/nanospheres and preparation method of tissue engineering scaffold

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preparation example Construction

[0022] The embodiment of the present invention also provides a preparation method for the above-mentioned tissue engineering, such as figure 1 shown, including the following steps:

[0023] S01: Dissolving the degradable synthetic material in a solvent and stirring for 24-48 hours to obtain a synthetic material solution;

[0024] S02: Add natural polymer microspheres and / or natural polymer nanospheres into the synthetic material solution, and stir for 24-72 hours to obtain a homogeneous slurry;

[0025] S03: Processing the mixed homogenate into shape and freeze-drying to obtain a tissue engineering scaffold;

[0026] Wherein, the mass ratio of the degradable synthetic material in step S01 to the natural polymer microspheres and / or natural polymer nanospheres in step S02 is (1:1)-(99:1).

[0027] The tissue engineering scaffold preparation method provided in this example can accurately control the ratio of natural materials and synthetic materials in the final scaffold due to...

Embodiment 1

[0038] This embodiment provides a tissue engineering scaffold, which specifically includes: (1) degradable synthetic material: 1.3g PLCL; (2) natural polymer microspheres: 0.0133g COLI microspheres (400 μm in diameter); COLI microspheres are uniformly dispersed in PLCL. The specific structure of the bracket is as follows figure 2 Shown: where a is the COLI microspheres in the scaffold; b is the vertical section view of the scaffold, the rectangle is PLCL, and the circle is COLI microspheres; c is the three-dimensional printing of the scaffold. From figure 2 It can be seen that COLI microspheres are uniformly dispersed in the synthetic material PLCL.

[0039] The preparation method of the above-mentioned tissue engineering scaffold material of the present embodiment is as follows: figure 1 As shown, the specific steps are:

[0040] S11: Weigh 1.3 g of PLCL material, add it into 10 mL of DIO solvent, place it on a magnetic stirrer and stir for 48 hours until the PLCL is co...

Embodiment 2

[0044] The present embodiment provides a tissue engineering scaffold, which specifically includes: (1) degradable synthetic material: 1 g of PLGA; (2) natural polymer nanospheres: 1 g of COLI nanospheres (600 nm in diameter); COLI nanospheres are uniform dispersed in PLGA. The specific structure of the bracket is as follows figure 2 Shown: where a is the COLI nanospheres in the scaffold; b is the vertical section view of the scaffold, the rectangle is PLGA, and the circle is COLI nanospheres; c is the three-dimensional printing of the scaffold. From figure 2 It can be seen that COLI nanospheres are uniformly dispersed in the synthetic material PLGA.

[0045] The preparation method of the above-mentioned tissue engineering scaffold material of the present embodiment is as follows: figure 1 As shown, the specific steps are:

[0046] S21: Weigh 1 g of PLGA material, add it into 10 mL of DIO solvent, place it on a magnetic stirrer and stir for 48 hours until PLGA is complete...

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Abstract

The invention discloses a tissue engineering scaffold containing natural high-molecular microspheres / nanospheres and a preparation method of the tissue engineering scaffold. The tissue engineering scaffold includes a degradable synthetic material and natural high-molecular microspheres and / or natural high-molecular nanospheres. The preparation method comprises the following steps: dissolving a degradable synthetic material in a solvent to obtain a synthetic material solution; adding natural high-molecular microspheres and / or natural high-molecular nanospheres into the synthetic material solution, and performing stirring to obtain a uniform mixed thick liquid; and processing the uniform mixed thick liquid and performing freeze drying to obtain the tissue engineering scaffold. Since the natural high-molecular microspheres and / or natural high-molecular nanospheres are uniformly dispersed in the synthetic material, the uniformity and biocompatibility of the scaffold are obviously improved.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a tissue engineering scaffold containing natural polymer microspheres / nanospheres and a preparation method thereof. Background technique [0002] At present, there are deficiencies in the cartilage repair technologies used clinically. The development of tissue engineering technology provides a new direction for the repair of cartilage damage, and plays an important role as a scaffold material, one of the three elements of tissue engineering. Therefore, the preparation of ideal tissue engineering scaffolds is one of the current research hotspots. [0003] The ideal cartilage scaffold material should have the characteristics of favorable cell adhesion and growth, good biocompatibility, degradability and appropriate degradation rate, highly porous three-dimensional structure, good mechanical properties and plasticity, etc. According to the difference of biological materials, m...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/56
CPCA61L27/50A61L27/56A61L2430/06
Inventor 王大平刘威何勇王大明黄江鸿陈洁琳段莉李文翠朱伟民熊建义
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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