Yarrowialipolytica yeast strain and application thereof

A technology of Yarrowia and strains, applied in the field of biotechnology microorganisms, can solve the problems of low fruit content, seasonal restrictions, high cost, etc.

Active Publication Date: 2017-11-10
浙江诺睿特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extremely low content in the fruit, the way of natural extraction has disadvantages such as high cost and seasonal restrictions

Method used

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  • Yarrowialipolytica yeast strain and application thereof
  • Yarrowialipolytica yeast strain and application thereof
  • Yarrowialipolytica yeast strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cultivation of Yarrowia lipolytica (Yarrowia lipolytica) NYL01 strain.

[0028] 1 medium

[0029] 1.1 YPD medium: glucose 2%, peptone 2%, yeast powder 1%, water 1L, pH 5.0-5.5. Add 2% agar powder to the solid medium on this basis, and adjust the pH to 6.0-6.5.

[0030] 1.2 Transformation medium: glucose 2%, yeast powder 1%, ricinoleic acid 10%, potassium dihydrogen phosphate 0.05, disodium hydrogen phosphate 0.03%, magnesium sulfate 0.01%, Tween 0.4%, water 1L. pH natural.

[0031] 2 Experimental methods

[0032] 2.1 Isolation, purification, screening and mutagenesis of high-yielding γ-decalactone strains

[0033] Step 1: Weigh an appropriate amount of soil samples from different soil samples, put them into a shaker bottle filled with sterile water, oscillate and shake for a while, take a small amount of soil leaching solution, dilute it with sterile water to different concentrations, and draw 50uL Spread on YPD solid medium. Incubate at 28°C for 2 days....

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Abstract

The invention belongs to the field of biotechnical microorganisms, and particularly relates to a Yarrowialipolytica (named as NYL01) bacterial strain for producing gamma-decalactone through bioconversion and application thereof. The strain is preserved in China Center for Type Culture Collection (CCTCC), the preservation address is China Wuhan University, the preservation number of CCTCCNO:M2015696, and the preservation date is 24th November, 2015. The strain has the advantages of being high in natural gamma-decalactone production, high in substrate conversion rate, simple in culture condition and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, in particular to a strain of Yarrowialipolytica (named as NYL01) for producing natural chiral gamma-decalactone by biotransformation and its application. Background technique [0002] γ-Decalactone is a ten-carbon compound containing a five-membered lactone ring. It has a strong peach and coconut aroma. It exists in a variety of fruits and dairy products. It is an internationally recognized safe food additive and pharmaceutical additive. Because of its attractive fragrance and low aroma threshold, it has been widely used in the fragrance industry. At present, γ-decalactone is mainly extracted from fruits or chemically synthesized. Due to the extremely low content in the fruit, the natural extraction method has disadvantages such as high cost and seasonal restrictions. The regulations on food additives and pharmaceutical additives strictly restrict the use of chemically synthesized pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P17/04C12R1/645
CPCC12P17/04C12N1/145C12R2001/645
Inventor 冯贻安沈情佳杨霄周俊辰
Owner 浙江诺睿特生物科技有限公司
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