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Kit for detecting lipase

A detection kit and lipase technology, which can be used in color/spectral characteristic measurement, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, etc., which can solve the problem of inability to cooperate with automatic biochemical analysis Instrument, low reaction accuracy, large substrate dependence, etc., to achieve the effect of increasing the measurable linear range, improving accuracy and sensitivity, and high accuracy

Active Publication Date: 2017-11-17
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PNPB method, pH-Stat method, and titration method cannot be used with automatic biochemical analyzers, and are not suitable for clinical diagnosis; most LPS detection kits currently use turbidimetric method, and the defect of this method is that it is highly dependent on the substrate And the accuracy of the reaction is not high, the repeatability is poor, and the linear range is narrow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The test kit provided by the invention is a single reagent, and the composition and ingredients of the reagent are as follows:

[0034] Acetic acid-magnesium acetate buffer solution 80mmol / L, pH=8.8, the pH value of this embodiment can also be 8.6 or 8.9;

[0035] Glyceryl trilaurate 100mmol / L

[0036] Oxidized coenzyme I (NAD + )0.2mmol / L

[0037] Glycerol dehydrogenase (GDH) 0.5KU / L

[0038] Preservative NaN 3 0.6g / L

[0039] Enzyme protection agent trehalose and sucrose both 2.5g / L

[0040] Developing agent Tween-80 (Tween-80) 0.1% and fatty alcohol polyoxyethylene (7) ether 0.3%

Embodiment 2

[0042] The test kit provided by the invention is a single reagent, and the composition and ingredients of the reagent are as follows:

[0043] Acetic acid-magnesium acetate buffer solution 120mmol / L, PH=8.8

[0044] Glyceryl trilaurate 200mmol / L

[0045] Oxidized coenzyme I (NAD + )0.3mmol / L

[0046] Glycerol dehydrogenase (GDH) 1.8KU / L

[0047] Preservative NaN 3 0.8g / L

[0048] Enzyme protection agent Trehalose and sucrose both 3.5g / L

[0049] Developing agent Tween-80 (Tween-80) 0.25% and fatty alcohol polyoxyethylene (7) ether 0.75%

Embodiment 3

[0051] The test kit provided by the invention is a single reagent, and the composition and ingredients of the reagent are as follows:

[0052] Acetic acid-magnesium acetate buffer solution 160mmol / L, PH=8.8

[0053] Glyceryl trilaurate 300mmol / L

[0054] Oxidized coenzyme I (NAD + )0.4mmol / L

[0055] Glycerol dehydrogenase (GDH) 3KU / L

[0056] Preservative NaN 3 1g / L

[0057] Enzyme protection agent trehalose and sucrose both 5g / L

[0058] Developing agent Tween-80 (Tween-80) 0.4% and fatty alcohol polyoxyethylene (7) ether 1.2%

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Abstract

The invention discloses a kit for detecting lipase. The kit is composed of a single reagent, the single reagent is prepared from a 80 mmol / L-160 mmol / L buffer system of which the PH is 8.8, 100 mmol / L-300 mmol / L glyceryl trilaurate, 0.2 mmol / L-0.4 mmol / L beta-nicotinamide adenine dinucleotide trihydrate, 0.5 KU / L-3 KU / L glycerol dehydrogenase, a 0.6 g / L-1 g / L preservative NaN3, a 5 g / L-10 g / L enzyme protective agent and a 0.4%-1.6% developing agent, and deionized water serves as a solvent. The kit for detecting the lipase has the advantages that samples do not need to be pretreated, a full-automatic biochemical analyzer can be directly utilized to conduct large-batch sample detection, and the kit is simple to operate, high in accuracy, good in repeatability, wide in linear range and suitable for clinical application popularization.

Description

technical field [0001] The invention relates to biological reagents, in particular to a detection kit, in particular to a lipase detection kit. Background technique [0002] Lipase (Lipase, glycerol ester hydrolase) belongs to the class of carboxyl ester hydrolase, which can gradually hydrolyze triglycerides into glycerol and fatty acids. It mainly comes from the pancreas, followed by the stomach and small intestine, and can hydrolyze a variety of glycerides containing long-chain (8-18 carbon chain) fatty acids. Lipase is synthesized in the acinar tissue of the pancreas and has high organ specificity. The increase of serum lipase concentration can be detected in the early stage of pancreatitis. For acute abdomen with high fatality rate, timely blood test for serum lipase is of great significance for diagnosis, treatment and prognosis. Early detection of serum lipase can better distinguish acute and non-acute pancreatitis, and its serum expression level has a significant po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 王贤俊张敏
Owner 王贤俊
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