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Resonance scattering spectrometry method for determining Hg<2+> by using ligand for adjusting and controlling activity of silicon dioxide nano-enzyme

A resonance scattering and silicon dioxide technology, applied in the field of analytical chemistry, can solve the problems that the analytical method has not been reported yet, and achieve the effect of simple method, stable system and high sensitivity

Inactive Publication Date: 2017-11-17
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a new nanomaterial, nano-silica is more and more widely used. The application of aptamers to regulate the catalysis of nano-silica non-metallic nanozymes and the application of resonance scattering spectroscopy to the quantitative determination of Hg 2+ Analytical methods have not yet been reported

Method used

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  • Resonance scattering spectrometry method for determining Hg&lt;2+&gt; by using ligand for adjusting and controlling activity of silicon dioxide nano-enzyme

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Embodiment

[0022] An aptamer-regulated activity of silica nanozyme-resonance scattering spectrometry for determination of Hg 2+ method, including the following steps:

[0023] (1) Prepare a known concentration of Hg 2+ Standard solution system: Add 10μL, 50μL, 100μL, 150μL, 200μL of 100nmol / L Hg into 7 graduated test tubes 2+ Standard solution, then add 100μL 66ng / mL mercury aptamer and 12μL 100μg / mL silicon dioxide to each graduated test tube, mix well, and let stand for 10 minutes; then add 150μL 0.5moL / L glucose, 150μL 0.01mol / L HCl and 100μL 84μmol / L HAuCl 4 , mix well, dilute to 1.5mL with twice distilled water, react in a water bath at 75°C for 20 minutes, take out the test tube, cool with ice water to terminate the reaction, and dilute to 2.0mL with twice distilled water;

[0024] (2) Prepare blank control solution system: do not add Hg with the method of step (1) 2+ Standard solution to prepare blank control solution system;

[0025] (3) Get the Hg prepared by steps (1), (2)...

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Abstract

The invention discloses a resonance scattering spectrometry method for determining Hg<2+> by using a ligand for adjusting and controlling activity of silicon dioxide nano-enzyme. The method is characterized by comprising the following steps: (1) preparing a Hg<2+> standard solution system I with known concentration; (2) preparing a blank control solution system; (3) calculating according to the equation that delta I is equal to I minus I0; (4) making a working curve according to a relationship between the delta I and Hg<2+> concentration; (5) preparing a to-be-measured sample solution, determining the resonance scattering peak intensity value of the to-be-measured sample solution as an I sample, and calculating according to the equation that delta I sample is equal to I sample minus I0; (6) calculating out the Hg<2+> content of the sample solution according to the working curve of step (4). Compared with the existing methods, the determining method of the invention does not need a complicated process of constructing a ligand nanoprobe, and the method is simpler and faster; the nanoparticles do not need to be aggregated and the system is more stable; the non-metal nano-enzyme catalysis has high sensitivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for regulating the activity of silica nanoenzymes with aptamers-resonance scattering spectroscopy to measure Hg 2+ Methods. Background technique [0002] Aptamer is a short oligonucleotide sequence obtained by in vitro screening, which can bind to a variety of target substances with high specificity, high affinity and high selectivity. When the nucleic acid aptamer specifically binds to the target substance, The configuration of the nucleic acid aptamer itself will change, and its detection signal will change accordingly to realize the detection of the target. Mercury is a highly toxic heavy metal. Mercury can easily accumulate in humans and animals, especially fish, through the food chain. Even at very low concentrations, mercury will cause great harm to humans and the environment. long-term toxic effects. Therefore, it is of great significance to study the determ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 李重宁梁爱惠蒋治良温桂清
Owner GUANGXI NORMAL UNIV