Resonance scattering spectrometry method for determining Hg<2+> by using ligand for adjusting and controlling activity of silicon dioxide nano-enzyme
A resonance scattering and silicon dioxide technology, applied in the field of analytical chemistry, can solve the problems that the analytical method has not been reported yet, and achieve the effect of simple method, stable system and high sensitivity
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[0022] A Kind of Using Aptamer to Regulate Silica Nanoenzyme Activity-Resonance Scattering Spectroscopy to Determine Hg 2+ The method includes the following steps:
[0023] (1) Prepare Hg of known concentration 2+ Standard solution system: In 7 graduated test tubes, add 10μL, 50μL, 100μL, 150μL, 200μL 100nmol / L Hg respectively 2+ Standard solution, then add 100μL of 66ng / mL mercury aptamer and 12μL of 100μg / mL silica to each graduated test tube, mix well, and let stand for 10 minutes; then add 150μL of 0.5moL / L glucose and 150μL to each test tube. 0.01moL / L HCl and 100μL 84μmoL / L HAuCl 4 , Mix well, dilute the volume to 1.5 mL with double distilled water, react in a water bath at 75°C for 20 minutes, take out the test tube, cool with ice water to stop the reaction, and dilute to 2.0 mL with double distilled water;
[0024] (2) Prepare a blank control solution system: use the method in step (1) without adding Hg 2+ Standard solution preparation blank control solutio...
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