A method for increasing fatty acid content in peony seeds
A fatty acid and peony technology, applied in the direction of fat production, fat oil/fat production, agriculture, etc., can solve the problems of dry and cracked grains, high cost, production reduction, etc., achieve high yield, maintain yield, and facilitate accumulation
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Embodiment 1
[0013] Embodiment 1 Fengdan seed morphology observation and moisture content determination
[0014] Peony enters the mixed bud germination stage in early March, and pollination occurs in mid-to-late April. The pods have begun to develop 10 days after pollination, and are basically formed by 40 days (see figure 1 ), but the seed kernels have been developed until 70 days before they are basically finalized (see figure 1 ); the size and shape of the seeds vary with the increase of the longitudinal and transverse diameters of the seeds (see figure 2 ), the pod weight is also constantly increasing and changing (see Table 1). According to the weight of fruit clips (10 pieces), 100-seed weight, longitudinal and transverse diameter of seeds in each period, the seed development process can be roughly divided into 4 periods: initial stage of seed development, period of rapid seed growth, period of slow seed growth and seed discoloration Expect.
[0015] The test materials were take...
Embodiment 2
[0023] The determination of fatty acid content of the seeds of embodiment 2 Fengdan
[0024] The test materials were taken from the seeds of healthy plants of the peony variety 'Fengdan' (Paeoniaostii T.Hong et J.X.Zhang) aged 7-8 years (planted in the experimental field of Qingdao Agricultural University in Shandong Province). After the peony bloomed, artificial pollination was carried out, and the seeds were bagged. For treatment, pods were taken 100 days after pollination, and then every 10 days until 130 DAP was terminated. The grains harvested at 100-130 DAP were stored at room temperature, and the peony seeds harvested at the same period without storage at room temperature were used for the determination of fatty acid content.
[0025] Preparation of standard solution
[0026] Preparation of internal standard solution: Dissolve 30 mg of methyl nonadecanoate (Methyl nonadecanoate) standard product in 10 mL of methanol to prepare a standard solution with a mass concentrat...
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