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A kit for detecting lung cancer

A kit and reagent technology, applied in the field of medical detection, can solve the problems of poor treatment effect, poor prognosis, and inability to detect large cell lung cancer early, and achieve the effect of rapid and effective detection

Active Publication Date: 2018-08-31
合肥金域医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if the large cell lung cancer can be diagnosed in the early stage of the disease, the treatment effect of the patient will be greatly improved, and the existing conventional detection methods cannot effectively detect the large cell lung cancer in the early stage, so it is urgent to provide an early diagnosis of large cell lung cancer. cell lung cancer approach

Method used

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  • A kit for detecting lung cancer
  • A kit for detecting lung cancer
  • A kit for detecting lung cancer

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Experimental program
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Effect test

Embodiment 1

[0013] Sample Collection and Ethics Statement

[0014] Blood was collected from 60 cases of large cell lung cancer and 30 cases of normal people. All samples were from the First Affiliated Hospital of Soochow University and the Second Affiliated Hospital of Soochow University. The samples were collected from September 2015 to June 2017. The collection of the above specimens was approved by the Ethics Review Committee of the First Affiliated Hospital of Soochow University and the Ethics Review Committee of the Second Affiliated Hospital of Soochow University, and all subjects signed the informed consent.

[0015] The above-mentioned patients with large cell carcinoma of the lung were obtained from serum specimens with cytological or pathological diagnosis through biopsy or surgery, and the patients had not undergone any radiotherapy or chemotherapy treatment before blood collection.

[0016] 1. Extraction of serum miRNA

[0017] 1) Add 500 μl TRIzol to 200 μl serum, vortex for...

Embodiment 3

[0040] Example 3 Fluorescent quantitative PCR detection of miRNA expression in gastric imprinted choriocarcinoma group and control group

[0041] 1) Fluorescence quantitative PCR

[0042] The kit uses FastFire qPCR PreMix (SYBR Green) from Tiangen Company. First, thaw FastFire qPCR PreMix, templates, primers, and RNase-free water, and dissolve all reagents at room temperature and mix thoroughly. Next, configure the fluorescent quantitative PCR system.

[0043] Fluorescent quantitative PCR system

[0044]

[0045] For miRNA expression detection, three parallel reactions were set up for each sample, and snRNA U6 was used as an internal reference.

[0046] The forward primer sequence in the above table is: ACACTCCAGCTGGGCCGGTTCCAGTCC (SEQ ID NO: 2).

[0047] The reverse primer sequence in the above table is: TGGTGTCGTGGAGTCG (SEQ ID NO: 3).

[0048] At the same time, U6 was used as an internal reference, and the forward primer was: GCTTCGGCAGCACATATACTAAAAT (SEQ ID NO: 5)...

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Abstract

The invention relates to a reagent kit for lung cancer detection. The reagent kit comprises a reagent for specific detection of miRNA (micro ribonucleic acid)-6070. The reagent kit can effectively detect a large cell lung cancer and quickly achieve quantitative detection of miRNA, and therefore achieves the purpose of quickly and effectively detecting the large cell lung cancer.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a kit for detecting lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. The cause of lung cancer is still not fully understood. According to the pathological analysis of the World Health Organization (WHO), lung cancer is divided into four main histological types: small cell lung cancer and non-small cell lung cancer, which can be further divided into lung adenocarcinoma, squamous cell carcinoma and large cell carcinoma. cancer. Large...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王振郭伟
Owner 合肥金域医学检验实验室有限公司
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