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Application of ul43 protein in the preparation of drugs for preventing and treating mitochondrial dysfunction

A dysfunctional and mitochondrial technology, applied in the field of protein research and application mechanism, can solve the problems of inhibiting virus cell-to-cell transmission, assembly, release, and affecting the endocytosis of viral envelope proteins, so as to increase the ATP content and broaden the application field.

Active Publication Date: 2020-06-05
GUANGZHOU HOPBIOYOUTH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ren and Melancon et al. showed that gK and UL20 also contain YXXΦ motifs, but when Y (tyrosine) is mutated to A (alanine), it will affect the endocytosis of viral envelope proteins, thereby inhibiting the virus cell-to-cell dissemination and assembly, release

Method used

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  • Application of ul43 protein in the preparation of drugs for preventing and treating mitochondrial dysfunction
  • Application of ul43 protein in the preparation of drugs for preventing and treating mitochondrial dysfunction
  • Application of ul43 protein in the preparation of drugs for preventing and treating mitochondrial dysfunction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 HSV-1 UL43 protein endocytosis and intracellular localization experiments

[0026] UL43 protein is a multi-transmembrane protein. After HSV-1 successfully infects host cells, UL43 protein can be expressed on the host cell membrane. Preliminary studies have shown that the amino acid sequence of UL43 protein contains an endocytic motif. Therefore, this example mainly explores whether the HSV-1 UL43 protein depends on the YXXΦ motif for endocytosis from the host cell membrane into the cell, and the intracellular localization of the UL43 protein.

[0027] Experimental Materials:

[0028] HSV-1 UL43 eukaryotic expression plasmid pHUL43, HSV-1 UL43 (Y144A) mutant expression plasmid pHUL43 (Y144A), Flag-labeled UL43 expression plasmid pCMV(f)UL43, Flag-labeled UL43 mutant plasmid pCMV(f ) UL43(Y144A). The eukaryotic expression vector pCDNA3.1+ was purchased from Invitrogen, and the plasmid pFLAG-CMV-2 was purchased from Sigma.

[0029] Herpes simplex virus F strai...

Embodiment 2

[0140] Example 2 Study on the Intracellular Function of HSV-1 UL43 Protein

[0141] It can be known from Example 1 that the main organ of UL43 multi-transmembrane protein in the host cell is the mitochondria, and the mitochondria are the energy supply organs of the cells, providing a large amount of ATP for the life activities of the cells. Therefore, this part mainly explores the influence of HSV-1 UL43 protein on the mitochondrial production pathway of host cells, and the research on the localization target of UL43 on mitochondria.

[0142] Table 3 main reagents

[0143] Experimental Materials source Glucose detection and analysis kit BioVision ATP Content Assay Kit Shanghai Kemin Biotechnology Co., Ltd. Pyruvate Kinase Kit Shanghai Baili Biotechnology Co., Ltd. Fructose-6-Phosphate Kinase Activity Assay Kit Shanghai Solaibao Biotechnology Co., Ltd. Lactate dehydrogenase kit Shanghai Baili Biotechnology Co., Ltd. SsoFas Eva...

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Abstract

The present invention provides applications of UL43 protein in preparing a drug for treating or preventing cellular mitochondrial dysfunction and in preparing a drug for treating or preventing metabolic syndrome.

Description

technical field [0001] The invention belongs to the field of protein research and application mechanism, more specifically, the invention relates to a UL43 protein that can be used as a drug for preventing and treating cell diseases. Background technique [0002] Herpes simplex virus (HSV) belongs to α-herpes virus, and the virus particle is a spherical particle with a diameter of 186 nm, such as Picture 1-1 As shown, the mature HSV mainly includes four components in morphology: the core includes the viral linear double-stranded DNA genome, which is wound into a fibril scroll; Composed of radially arranged capsomers with hollow shaft holes, it has the function of protecting the virus genome; the capsid is surrounded by a layer of loose protein region, which is uneven in thickness, called the capsule; the outermost layer is a typical lipid-containing The capsule of the double-membrane structure has protrusions on it and contains a large number of envelope glycoproteins. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P3/00
CPCA61K38/162
Inventor 孙晗笑利时雨张倩刘照兵刘梦鸽黄金群
Owner GUANGZHOU HOPBIOYOUTH BIOTECH CO LTD
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