Method for synthesizing D-psicose by combining phosphonase and ATP regeneration system

Abstract

The invention discloses a method for improving the equilibrium conversion rate of D-psicose synthesized by taking D-glucose as a substrate through combination of phosphonase and an ATP regeneration system. D-psicose is prepared by taking D-glucose as a raw material through recombinant escherichia coli construction, wet thallus preparation and whole-cell catalytic reaction in sequence. The recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phospho-3 epimerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase, and the recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase. Wherein the construction process of the recombinant escherichia coli comprises introduction of an ATP regeneration system. According to the method, phosphoenzyme and an ATP regeneration system are combined, so that the conversion rate of synthesizing D-psicose from D-glucose is increased, and the addition of exogenous ATP is reduced. The method has the characteristics of simple preparation process, high product conversion rate, greenness and economy, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the technical field of synthetic biology, in particular to a method for synthesizing D-psicose by combining phosphatase and ATP regeneration system. Background technique [0002] D-psicose is the epimer of D-fructose at the C-3 position. It is rare in nature, with a sweetness of 70% of sucrose and only 0.3% of energy. At the same time, D-psicose has been rated as "GRAS" by the U.S. Food and Drug Administration, allowing it to be added to foods, dietary supplements and pharmaceutical preparations. D-psicose has many special functions that are beneficial to human health. For example, taking an appropriate amount of D-psicose can reduce blood sugar and blood lipid levels, reduce fat accumulation, and scavenge reactive oxygen species. attention, and developing a method for efficient production of D-psicose has become a necessary condition to meet the market growth. [0003] In recent years, most researchers use D-psicose 3-epimer...

AI Technical Summary

Problems solved by technology

Although this method improves the conversion efficiency, because it requires two steps, the operation in actual production is more cumbersome and takes a long time, which affects the production efficiency.

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