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Method for synthesizing D-psicose by combining phosphonase and ATP regeneration system

A technology of psicose and phosphoglucosidase, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as cumbersome operation, affecting production efficiency, and long time, and achieve operational Convenience, reduced preparation cost, simple and convenient operation

Pending Publication Date: 2022-07-08
湖南成大生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method improves the conversion efficiency, because it requires two steps, the operation in actual production is more cumbersome and takes a long time, which affects the production efficiency.

Method used

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  • Method for synthesizing D-psicose by combining phosphonase and ATP regeneration system
  • Method for synthesizing D-psicose by combining phosphonase and ATP regeneration system
  • Method for synthesizing D-psicose by combining phosphonase and ATP regeneration system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0040] During the whole-cell catalytic reaction, the temperature of the entire reaction system was 35° C., and the pH was 7.0, and other operations were the same as those in Example 1.

Embodiment 3

[0042] During the whole-cell catalytic reaction, the temperature of the entire reaction system was 37° C., the pH was 7.5, and ATP was added in an amount of 30 mM, and other operations were the same as those in Example 1.

Embodiment 4

[0044] During the whole-cell catalytic reaction, the temperature of the entire reaction system was 40° C., the pH was 8.0, and the rest of the operations were the same as those in Example 1.

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PUM

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Abstract

The invention discloses a method for improving the equilibrium conversion rate of D-psicose synthesized by taking D-glucose as a substrate through combination of phosphonase and an ATP regeneration system. D-psicose is prepared by taking D-glucose as a raw material through recombinant escherichia coli construction, wet thallus preparation and whole-cell catalytic reaction in sequence. The recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phospho-3 epimerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase, and the recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase. Wherein the construction process of the recombinant escherichia coli comprises introduction of an ATP regeneration system. According to the method, phosphoenzyme and an ATP regeneration system are combined, so that the conversion rate of synthesizing D-psicose from D-glucose is increased, and the addition of exogenous ATP is reduced. The method has the characteristics of simple preparation process, high product conversion rate, greenness and economy, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the technical field of synthetic biology, in particular to a method for synthesizing D-psicose by combining phosphatase and ATP regeneration system. Background technique [0002] D-psicose is the epimer of D-fructose at the C-3 position. It is rare in nature, with a sweetness of 70% of sucrose and only 0.3% of energy. At the same time, D-psicose has been rated as "GRAS" by the U.S. Food and Drug Administration, allowing it to be added to foods, dietary supplements and pharmaceutical preparations. D-psicose has many special functions that are beneficial to human health. For example, taking an appropriate amount of D-psicose can reduce blood sugar and blood lipid levels, reduce fat accumulation, and scavenge reactive oxygen species. attention, and developing a method for efficient production of D-psicose has become a necessary condition to meet the market growth. [0003] In recent years, most researchers use D-psicose 3-epimer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12N15/70C12N15/54C12N15/61C12N15/55C12R1/19
CPCC12P19/02C12N15/70C12N15/52C12N9/1205C12N9/92C12N9/90C12N9/16C12Y207/01002C12Y503/01009C12Y501/03C12Y301/03
Inventor 刘继栋李宏伟郭燕王晖怡李秋凤
Owner 湖南成大生物科技有限公司
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