Method for extracting raspberry ketone glucoside and ellagic acid from rubus corchorifolius

A technology of raspberry glycosides and ellagic acid, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of less extraction of medicinal ingredients and neglect of medicinal value, and reduce production cost, increase yield, and simplify the production process

Inactive Publication Date: 2017-12-01
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on it mainly focuses on resource investigation, but there are few research reports on the extraction of its medicinal components and pharmacology, especially the medicinal value of its fruit has not been paid attention to.

Method used

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Embodiment approach

[0034] Below, the present invention will be further described with embodiment, but it is not limited to any of these embodiments

[0035] one or similar instance.

Embodiment 1

[0037] Homogenize 100kg of raspberry fruit, add 7kg of Bacillus subtilis powder CGMCC No.1.769, stir and keep warm at 30°C for 12h, add 300L of 60% ethanol aqueous solution to mix, reflux at 55°C for 60min, filter with ceramic membrane One time, get 350L extract. Concentrate the extract under reduced pressure at 50°C to 20L to obtain concentrated solution I, absorb the concentrated solution on AB-8 macroporous resin, wash with three times the column volume of water, and use four times the column volume of ethanol containing 35% ethanol Elute with 0.001mol / L HCL ethanol aqueous solution to obtain 80L eluent I; then use five times column volume of 0.00001mol / L HCL ethanol aqueous solution containing 85% ethanol to elute AB-8 macroporous resin to obtain 100L Eluate II, and concentrate the eluate II under reduced pressure at 50°C to 5L to obtain concentrate II; concentrate the eluate I at 45-55°C under reduced pressure to 7L, and adjust the pH to 3 with 0.1mol / L HCL , stand overn...

Embodiment 2

[0039] Homogenize 200kg of raspberry fruit, add 15kg of Bacillus subtilis powder CGMCC No.1.769, stir and keep warm at 32°C for 15h, add 600L of 60% ethanol aqueous solution to mix, reflux at 55°C for 60min, filter with ceramic membrane Once, get 640L extract. Concentrate the extract to 40L under reduced pressure at 50°C to obtain the concentrated solution I. The concentrated solution is adsorbed on KLFC-150 macroporous resin, washed with three times the column volume of water, and washed with three times the column volume of ethanol containing 35% ethanol. Elute with 0.001mol / L HCL ethanol aqueous solution to obtain 120L eluent I; then use four column volumes of 0.00001mol / L HCL ethanol aqueous solution containing 75% ethanol to elute KLFC-150 macroporous resin to obtain 150L Eluate II, and concentrate eluate II under reduced pressure at 45-55°C to 12L to obtain concentrate II; concentrate eluate I at 50°C under reduced pressure to 10L, and adjust the pH to 3 with 0.1mol / L HC...

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Abstract

The invention provides a method for extracting raspberry ketone glucoside and an ellagic acid from rubus corchorifolius. A raspberry ketone glucoside and ellagic acid pure product is prepared by fresh rubus corchorifolius fruits through the steps of water extraction, alcohol extraction, macroporous resin adsorption, sub-step elution and concentrated crystallization. The method is simply operated, and is capable of greatly improving the yield of the raspberry ketone glucoside and the ellagic acid, greatly reducing the production cost of the raspberry ketone glucoside and the ellagic acid, and simplifying the production process. The rubus corchorifolius is used as the extracting raw material, the utilization efficiency of the rubus corchorifolius is greatly improved, and the additional value of the rubus corchorifolius is improved.

Description

Technical field [0001] The invention relates to a method for extracting raspberry glucoside and ellagic acid from fresh raspberry fruit, belonging to the technical field of natural product separation. Background technique [0002] Raspberry has another name called raspberry, mountain throwing seed, milk bubble, sowing seedling bubble, March bubble, April bubble, dragon boat bubble, a plant of the genus Rubus in the Rosaceae. Distributed in Japan, Vietnam, North Korea, Myanmar, and Gansu, Tibet, Northeast China, Qinghai, Xinjiang and other places in mainland China, it grows in areas with an altitude of 200 meters to 2,200 meters, mostly in sunny hillsides, valleys, wastelands, streams and dredging Damp places in dense shrubs have not yet been artificially introduced and cultivated. Deciduous shrub, 1-2 meters high, branchlets reddish brown, with many prickles distributed on the branches, young branches greenish, pubescent and prickles. Leaves are ovate or ovate-lanceolate, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/06C07H15/203C07H1/08
CPCC07D493/06C07H1/08C07H15/203
Inventor 罗婵
Owner 长沙爱扬医药科技有限公司
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