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Extraction method of organic acid in asparagus rhizosphere soil

A technology of rhizosphere soil, extraction method, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2017-12-01
VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for extracting organic acids in asparagus rhizosphere soil, so as to solve the lack of a method for effectively extracting organic acids in the specific environment of asparagus rhizosphere soil in the prior art. technical issues of the method

Method used

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  • Extraction method of organic acid in asparagus rhizosphere soil

Examples

Experimental program
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Effect test

Embodiment 1

[0032] ① Germination of asparagus Jinggang 701 and Jingganghong varieties: the selected asparagus seeds are cleaned and then disinfected with sodium hypochlorite. Soak the seeds in sodium hypochlorite, stir constantly, and rinse with water after 5 minutes.

[0033] ② Soak the seeds in warm water at 25-30°C for 36-48 hours, and change the water 2-3 times a day.

[0034] ③ Wash the soaked seeds and place them in a petri dish with moist filter paper, place them in an environment of 25-30°C for 48-72 hours to germinate, and rinse them with water 1-2 times a day.

[0035] ④ Sow the germinated and dewy seeds into the nutrient bowl, the ratio of soil to substrate is 3:1.

[0036] ⑤Pretreatment steps of ion exchange resin

[0037] The pretreatment steps of strong acid cation exchange resin are as follows:

[0038] A. First soak in clean water for 24 hours, rinse the resin until the water is clear and free of turbidity and impurities.

[0039] B. Stir with 3 times the amount of 1M h...

Embodiment 2

[0053] A method for extracting organic acids in asparagus rhizosphere soil, comprising the following steps:

[0054] 1) In a 250mL Erlenmeyer flask, mix 30g of asparagus rhizosphere soil with 100mL of ultrapure water, seal the mouth of the bottle, shake on a shaker for 2 hours, then filter with two layers of qualitative filter paper, and take the filtrate for later use;

[0055] 2) Pass the filtrate obtained in step 1) through a cation exchange resin column and an anion exchange resin column successively at a flow rate of 0.6mL / min, and then elute the organic acid component retained in the anion exchange column with a 1mol / L HCl solution , the column flow rate during elution is 0.5mL / min;

[0056] 3) Concentrate the eluate with a rotary evaporator at a temperature of 35°C and a speed of 80r / min to remove the liquid phase components, wash out the residue with ultrapure water and set the volume to 10mL, and filter with a water-based filter membrane with a pore size of 0.22μm , ...

Embodiment 3

[0073] A method for extracting organic acids in asparagus rhizosphere soil, comprising the following steps:

[0074] 1) In a 250mL Erlenmeyer flask, mix 30g of asparagus rhizosphere soil with 100mL of ultrapure water, seal the mouth of the bottle, shake on a shaker for 2 hours, then filter with two layers of qualitative filter paper, and take the filtrate for later use;

[0075] 2) Pass the filtrate obtained in step 1) through a cation exchange resin column and an anion exchange resin column successively at a flow rate of 0.6mL / min, and then elute the organic acid component retained in the anion exchange column with a 1mol / L HCl solution , the column flow rate during elution is 0.5mL / min;

[0076] 3) Concentrate the eluent with a rotary evaporator at a temperature of 35°C and a speed of 90r / min to remove the liquid phase components, wash out the residue with ultrapure water and set the volume to 10mL, and filter with a water-based filter membrane with a pore size of 0.22μm , ...

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Abstract

The invention provides an extraction method of organic acid in asparagus rhizosphere soil. According to the technical scheme which is specific to the physical characteristics of soil and the ingredient characteristics of asparagus rhizosphere autotoxicity organic acid, an appropriate extraction scheme is designed. According to the method, the asparagus rhizosphere soil obtained via sampling and ultrapure water are mixed, soluble matter can be dissolved out from the soil by using a vibrating method, impurities are removed by using a filtration method, and obtained filtrate contains organic acid to be tested. On the basis, a cation exchange resin column and an anion exchange resin column are successively utilized by the method to perform chromatographic separation, the organic acid content to be tested in obtained eluate is fully promoted, finally, rotary evaporation condensation is conducted at relatively low temperature, a purified product is obtained on the basis of guaranteeing that an organic acid ingredient to be tested is stable, and the product can be used as a sample to be tested after being subjected to constant volume by using the ultrapure water so as to utilize HPLC (high performance liquid chromatography) to perform quantitative analysis.

Description

technical field [0001] The invention relates to the technical field of asparagus cultivation, and further relates to the separation and extraction technology of asparagus autotoxic substances in the cultivation process, in particular to a method for extracting organic acids in asparagus rhizosphere soil. Background technique [0002] Asparagus (Asparagus officinalis L.), also known as Asparagus officinalis L., is a perennial herbaceous plant belonging to the Liliaceae Asparagus genus. Its tender stems are tender and delicious, rich in nutrition and unique in flavor. It is a high-end vegetable that is loved by consumers and is known as the "king of vegetables". Asparagus is rich in saponins, flavonoids and plant polysaccharides, etc. The active ingredient has good anti-tumor, anti-oxidation and blood pressure-lowering effects. In recent years, as domestic consumers have become more aware of the nutritional value and unique medicinal effects of asparagus, the consumption in t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/12
CPCG01N30/02G01N30/12G01N2030/126
Inventor 尹玉玲谢启鑫陈光宇周劲松罗绍春汤泳萍张岳平
Owner VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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