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Calcutta hemp InDel molecular marker as well as development method and application thereof

A molecular marker and jute technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA / RNA fragments, etc., to achieve the effect of high polymorphism, clear bands, and rich genetic basic information

Active Publication Date: 2017-12-08
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Techniques for developing jute InDel markers using multiple transcriptome data have not been reported yet

Method used

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  • Calcutta hemp InDel molecular marker as well as development method and application thereof
  • Calcutta hemp InDel molecular marker as well as development method and application thereof
  • Calcutta hemp InDel molecular marker as well as development method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0050] Using Trinity splicing software to conduct bioinformatics analysis on 188.66Gb of sequencing data (from the inventor's laboratory for NY, TC long fruit jute sequencing data and the round fruit 179 sequencing data downloaded from the Internet), splicing a set of reference transcripts

[0051] Using the bioinformatics analysis software GATK3, the sequencing data of NY and TC Changguo jute and the sequencing data of Yuanguo 179 downloaded from the Internet were located on the reference transcript spliced ​​in (1), and the InDel site was found, and the Three material (variety) genotypes.

[0052] The software developed by the inventor's laboratory was used to analyze the size of the differential fragments of NY, TC and 179InDel.

[0053] NY, TC and Yuanguo 179 DNA were extracted, and the developed InDel marker polymorphism was verified experimentally.

[0054] 22 jute germplasm resources were genotyped with 29 pairs of polymorphic primers, and phylogenetic cluster analysis...

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Abstract

The invention relates to the technical field of a molecular marker, in particular to a calcutta hemp InDel molecular marker as well as a development method and application thereof. The molecular markers can be used for identifying the allele polymorphism, identifying identical or relevant calcutta hemp plants, distinguishing the calcutta hemp plants and studying the genetic diversity in populations. The molecular marker can also be used for using inheritance and phenotype study of a statistical method, such as linkage analysis, QTL positioning, genealogy confirmation, population genetic polymorphism study, association mapping and linkage disequilibrium; the information can be used for calcutta hemp plant propagation and / or selection.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a jute InDel molecular marker and its development method and application. Background technique [0002] Jute is an annual bast fiber crop of the genus Corchorus in the Tiliaceae family, and is an important raw material for the hemp spinning and papermaking industries. The genus Jute has about 40 species and is found throughout the tropics and subtropics. China is the oldest jute producing country, and the yield per unit area of ​​jute ranks first in the world. [0003] Molecular markers have been widely used in crop research, such as variety identification, genetic diversity analysis, evolutionary analysis, linkage map construction, comparative genomics, quantitative trait locus mapping, molecular assisted breeding selection, etc. There are many molecular markers developed for basic and applied research, such as restriction fragment length polymorphism (RFLP), amplifi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 杨泽茂粟建光戴志刚陈基权唐蜻许英程超华刘婵
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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