Method for preparing single nanopore interface on glass substrate
A glass substrate and nanopore technology, applied in the field of preparing a single nanopore interface, can solve the problems of high cost and complicated operation.
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Embodiment 1
[0046] A method for preparing a single nanopore interface on a glass substrate. The method is to set an etching solution containing HF on one side of the glass substrate. The etching solution covers at least one etching area, and the etching area is prepared for a single The area where the nanoholes are located, that is to say, the etched area corresponds to the projection area of the prepared single nanohole on the surface of the glass substrate, corresponding to the alkaline electrolyte solution provided on the other side of the etched area, that is to say Set an alkaline electrolyte solution on the other side of the glass substrate corresponding to the other side where a single nanopore is to be prepared. The alkaline electrolyte solution also covers at least one etching area; the etching solution and the alkaline electrolyte solution are applied with a DC power supply The etching is performed with a direct current voltage, and a galvanometer is used to monitor the etching ...
Embodiment 2
[0055] This embodiment is based on embodiment 1 using figure 1 The device shown uses an electric field assisted hydrofluoric acid wet etching of the capillary wall to prepare a single hole and a method of characterization. The specific implementation method is as follows:
[0056] 1. Take a 5.0cm-long quartz capillary tube 2 coated with polyimide coating 1 on the outer wall (the inner diameter of the capillary can be 50-250μm), and peel off the polyimide coating of 1mm in the middle of the surface of the capillary 2 as an engraving Eclipse area 3. Fix this capillary 1 on a 2.0mm×7.5mm glass slide 4, stick a 1cm×1cm transparent tape on the middle of the slide 4, and place the etched area of the capillary wall in the middle of the transparent tape area. The reservoirs (A, B, C) at both ends of the capillary and the middle etched part are fixed with epoxy resin 5.
[0057] 2. Pour 0.05 moL / L Tris-HCl alkaline electrolyte solution 6 into the reservoirs (A, C) and capillaries at bot...
Embodiment 3
[0061] This example shows the concentration polarization effect on the single-hole interface prepared in Example 2, and further confirms that the size of the openings is at the nanometer level based on the SEM as in Example 2. Fill the tube with 1μg / mL ethidium bromide (EB) and 3.1ng / μL double-stranded DNA (dsDNA) 1×TBE buffer solution, then apply voltage to the interface, connect the positive electrode (+) on the outside, and connect the negative electrode in the capillary (-), observe the concentration polarization behavior of EB-labeled fluorescent dsDNA molecules on this interface under the action of an electric field by inverted microscopic fluorescence imaging. After EB binds to dsDNA, it appears red under the excitation of green light. Its structure diagram is as Figure 5 As shown, under the driving of an electric field with a voltage of 100v, at the beginning, the fluorescent probe dsDNA molecules labeled with EB cannot be seen in the capillary, and within 50s, the neg...
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