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Rapid detection method for staphylococcus aureus

A staphylococcus and detection method technology, which is applied in the field of microbial detection, can solve the problems of high cost of use, and achieve the effects of low cost, simplified color development steps, and shortened culture time

Inactive Publication Date: 2017-12-12
HENAN COOP MEDICAL SCI & TECH INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cost of this invention is lower than that of the former, its use cost is still relatively high for occasions requiring a large number of detections

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A rapid detection method for staphylococcus aureus, comprising the following steps:

[0020] 1) Bacteria enrichment: add the sample to be tested into normal saline to make a homogeneous sample solution with a sample mass concentration of 15%; dissolve the bacterium enrichment agent in water at a mass ratio of 1:150 to obtain a bacterium enrichment solution; The homogeneous solution and the enrichment solution were mixed at a volume ratio of 1:5, and cultured with shaking at 37°C for 4 hours to obtain the sample enrichment solution;

[0021] 2) Heat treatment: Pour the test solution into a sterilized Petri dish with a liquid level of 0.3cm, and incubate at 62±2°C for 1 hour;

[0022] 3) Cultivation and color development: Take 1mL of the heat-treated test solution, spread it evenly in the color development medium, incubate at 35±2°C for 16 hours, observe the color change of the color development film, if a pink halo appears, it means Staphylococcus aureus was present in ...

Embodiment 2

[0026] A rapid detection method for Staphylococcus aureus, comprising the following steps:

[0027] 1) Bacteria enrichment: Add the sample to be tested into normal saline to make a homogeneous sample solution with a sample mass concentration of 15%; dissolve the bacterium enrichment agent in water at a mass ratio of 1:200 to obtain a bacterium enrichment solution; The homogeneous solution and the enrichment solution were mixed at a volume ratio of 1:5, and cultured with shaking at 37°C for 5 hours to obtain the sample enrichment solution;

[0028] 2) Heat treatment: Pour the test solution into a sterilized Petri dish with a liquid level of 0.8cm, and incubate at 62±2°C for 1.5h;

[0029] 3) Cultivation and color development: Take 1 mL of the heat-treated test solution, spread it evenly on the color development medium, incubate at 35±2°C for 20 h, observe the color change of the color development chip, if a pink halo appears, it means Staphylococcus aureus was present in the s...

Embodiment 3

[0033] A rapid detection method for Staphylococcus aureus, comprising the following steps:

[0034] 1) Bacteria enrichment: add the sample to be tested into normal saline to make a sample homogeneous solution with a sample mass concentration of 15%; dissolve the bacterium enrichment agent in water at a mass ratio of 1:180 to obtain a bacterium enrichment solution; The homogeneous solution and the enrichment solution were mixed at a volume ratio of 1:5, and cultured with shaking at 37°C for 4.5 hours to obtain the sample enrichment solution;

[0035] 2) Heat treatment: Pour the test solution into a sterilized Petri dish with a liquid level of 0.6cm, and incubate at 62±2°C for 1.2h;

[0036] 3) Cultivation and color development: take 1mL of the heat-treated test solution, spread it evenly in the color development medium, incubate at 35±2°C for 18 hours, observe the color change of the color development film, if a pink halo appears, it means Staphylococcus aureus was present in ...

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Abstract

The invention relates to a rapid detection method for staphylococcus aureus and belongs to the technical field of microbiological detection. The rapid detection method comprises the following steps: proliferating bacteria, carrying out heat treatment, culturing, and developing. The detection method provided by the invention has the advantages that the aim of rapid detection can be achieved by shortening culture time and simplifying the developing step, and cost is relatively low, so that the rapid detection method provided by the invention can be applicable to mass detection.

Description

technical field [0001] The invention relates to a rapid detection method for Staphylococcus aureus, belonging to the technical field of microbial detection. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is an important pathogenic bacterium of humans, which belongs to the genus Staphylococcus (Staphylococcus). [0003] Staphylococcus aureus is ubiquitous in nature and can be found in air, water, dust, and human and animal waste. Therefore, there are many opportunities for food to be contaminated. The U.S. Centers for Disease Control reports that Staphylococcus aureus is the second-leading infection after E. coli. Staphylococcus aureus enterotoxin is a worldwide health problem. In the United States, food poisoning caused by Staphylococcus aureus enterotoxin accounts for 33% of the total bacterial food poisoning, and Canada has more, accounting for 45%. Food poisoning incidents caused by cocci also occur from time to time. [0004] In order to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12N1/20C12R1/445
CPCC12N1/20C12Q1/14
Inventor 张相生吴丽侯银臣黄继红景黎霞巴欣毅张嘉晨王春阳周俊娇
Owner HENAN COOP MEDICAL SCI & TECH INST CO LTD
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