Unlock instant, AI-driven research and patent intelligence for your innovation.

Aspergillus ZJUBE 2010 and application thereof

A technology of ZJUBE2010 and marine Aspergillus niger, which is applied to marine Aspergillus niger ZJUBE2010 and its application fields, can solve the problems of high production cost, high manufacturing cost of fermentation equipment, and large energy consumption, so as to reduce equipment manufacturing cost, reduce energy consumption, High conversion effect

Pending Publication Date: 2017-12-19
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of marine Aspergillus niger ZJUBE 2010 and its application, aiming to solve the problem of high manufacturing cost of fermentation equipment, high production cost and large energy consumption caused by the need to sterilize the fermentation substrate in the existing solid-state fermentation process question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of spore culture:

[0027] Spore culture medium: adopt potato dextrose agar medium PDA (200 grams of potatoes, 20 grams of glucose, 20 grams of agar powder, and add distilled water to 1 liter), divide the medium into test tubes in 5 milliliters, and sterilize at 121 ° C. After 20 minutes of sterilizing, spread the test tube on a slope until it cools and solidifies.

[0028] The marine Aspergillus niger ZJUBE 2010 was inoculated on the spore medium with an inoculation loop, and cultured at 30°C for 5 days.

[0029] The marine Aspergillus niger ZJUBE 2010 spores were rinsed with 50ml of sterile distilled water and transferred to a 150ml Erlenmeyer flask filled with glass beads, and shaken on a shaker at 180-200rpm for 1 hour to disperse the spores evenly.

[0030] (2) Preparation of seeds

[0031] Seed medium: 1% glucose, 0.25% yeast extract, 0.25% peptone, 1% available carbon source and 0.25% organic nitrogen source, natural pH.

[0032] Inoculation: A...

Embodiment 2

[0041] (1) Preparation of spore culture:

[0042] Spore culture medium: adopt potato dextrose agar medium PDA (200 grams of potatoes, 20 grams of glucose, 20 grams of agar powder, and add distilled water to 1 liter), divide the medium into test tubes in 5 milliliters, and sterilize at 121 ° C. After 20 minutes of sterilizing, spread the test tube on a slope until it cools and solidifies.

[0043] The marine Aspergillus niger ZJUBE 2010 was inoculated on the spore medium with an inoculation loop, and cultured at 35°C for 3 days.

[0044] The marine Aspergillus niger ZJUBE 2010 spores were rinsed with 50ml of sterile distilled water and transferred to a 150ml Erlenmeyer flask filled with glass beads, and shaken on a shaker at 180-200rpm for 1 hour to disperse the spores evenly.

[0045] (2) Preparation of seeds

[0046] Seed medium: 1% glucose, 0.25% yeast extract, 0.25% peptone, 5% available carbon source and 3% organic nitrogen source, natural pH.

[0047] Inoculation: Adju...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses aspergillus ZJUBE 2010 and an application thereof. The aspergillus ZJUBE 2010 is preserved in China Center for Type Culture Collection on 2nd, June, 2010 with a preservation number of CCTCC NO: M2010132. The aspergillus ZJUBE 2010 is used for producing cellulase through fermentation, which has the advantages of high output and good enzyme activity; in addition, the aspergillus ZJUBE 2010 can be used for fermentation and degradation of a charcoal source, the monosaccharide conversion rate is high, high-temperature and high-pressure disinfection processing is not required for a raw material fermentation medium and the equipment during a fermentation process, the fermentation effect is not influenced, high manufacture cost of the equipment is effectively reduced, production cost is reduced, and the energy consumption is reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a marine Aspergillus niger ZJUBE 2010 and its application. Background technique [0002] Aspergillus niger produces cellulase under the premise of using cellulose as an inducer, which is used to degrade cellulose into available monosaccharides. The most commonly used fermentation substrates in the fermentation of Aspergillus niger are wheat bran, corn straw, and rice straw powder, which can be used as the main carbon source and inducer. [0003] The commonly used fermentation methods of Aspergillus niger include liquid fermentation and solid state fermentation. Among them, there is almost no free water in solid-state fermentation, which is closer to the natural growth state of microorganisms, with higher enzyme activity, simple follow-up treatment, less pollution, and no waste water discharge; and the solid-state fermentation method is more extensive, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12P19/02C12R1/685
CPCC12N9/2437C12P19/02C12N1/145C12R2001/685
Inventor 郑刚魏丹华张澄杨志坚姚善泾泮红文陈磊
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV