Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound fungicide for nitrogen removal by denitrification, preparation method and application thereof

A nitrification and denitrification composite bacterial agent and composite bacterial agent technology are applied in the field of environmental bioengineering and water treatment, which can solve the problems of increased sewage treatment costs, unstable treatment effects, and low denitrification rate, and achieve improved degradation rates and COD High removal rate, convenient storage and transportation, high nitrate nitrogen degradation rate and high COD removal rate

Active Publication Date: 2017-12-19
河南中顺环保科技有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Biological denitrification technology is the use of microbial denitrification to remove pollutants such as nitrate nitrogen and nitrite nitrogen in wastewater. At present, there are problems such as low denitrification rate and poor treatment efficiency.
In order to solve this problem, the denitrification treatment effect is usually improved by reducing the treatment load, increasing the sewage treatment time, and improving the sludge settling property. However, the treatment effect of these measures is very unstable, and it will also increase the sewage treatment cost and increase economic pressure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound fungicide for nitrogen removal by denitrification, preparation method and application thereof
  • Compound fungicide for nitrogen removal by denitrification, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A denitrification and denitrification composite bacterial agent is prepared from the following bacterial strains in volume percentage: 20% of Paracoccus, 20% of Paleobacterium pallidus, and 60% of Achromobacter.

[0035] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0036] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0037] 1) Two rings of Paracoccus, Paleobacterium and Achromobacter that were preserved on the slant were respectively inoculated in solid medium for culture, the culture temperature was 25°C, and the culture time was 3 days, and Paracoccus, Paleobacterium and Achromobacter were obtained respectively Activated bacteria;

[0038] 2) Divide the enrichment medium into 250mL Erlenmeyer fla...

Embodiment 2

[0052] A denitrification and denitrification composite bacterial agent is prepared from the following bacterial strains in volume percentage: 25% of Paracoccus, 25% of Paleobacterium pallidus, and 50% of Achromobacter.

[0053] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0054] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0055] 1) Two rings of Paracoccus, Paleobacterium and Achromobacter were inoculated on the solid medium respectively, cultured at a temperature of 30°C and cultured for 2 days to obtain Paracoccus, Paleobacter and Achromobacter respectively. Activated bacteria;

[0056] 2) Divide the enrichment medium into 250mL Erlenmeyer flasks, 70mL / bottle; take two rings of the activated strains of...

Embodiment 3

[0070] A denitrification and denitrification composite bacterial agent is prepared from the following bacterial strains in volume percentage: 30% of Paracoccus, 30% of Paleobacterium pallidus, and 40% of Achromobacter.

[0071] As mentioned, the number of live bacteria of Paracoccus is 1×10 7 -1×10 9 CFU / g; the number of viable bacteria of the pale bacillus is 1×10 7 -1×10 10 CFU / g; the number of viable bacteria of the achromobacter is 1×10 5 -1×10 9 CFU / g.

[0072] A preparation method of a denitrification and denitrification compound bacterial agent is prepared by the following steps:

[0073] 1) Take two rings each of Paracoccus, Paleobacterium and Achromobacter that were preserved on the slant, and inoculate them in solid medium for culture. The culture temperature was 35°C and the culture time was 1 day, and Paracoccus, Paleobacterium and Achromobacter were obtained respectively Activated bacteria;

[0074] 2) Divide the enrichment medium into 250mL Erlenmeyer flas...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a compound fungicide for nitrogen removal by denitrification. The compound fungicide is prepared from the following bacteria by volume percent: 20-30% of paracoccus, 20-30% of ochrobactrum and 40-60% of achromobacter. The invention also discloses a preparation method for the compound fungicide for nitrogen removal by denitrification and an application thereof in treating sewage containing nitrate nitrogen. The compound fungicide for nitrogen removal by denitrification disclosed by the invention has excellent properties of high adaptability, short treating time, high degradation rate of nitrate nitrogen and high removal rate of COD under the synergistic effect of paracoccus, ochrobactrum and achromobacter; when the compound fungicide is applied to the sewage, the degradation rate of nitrate nitrogen and the removal rate of COD in the sewage are obviously increased; the cost is greatly saved; and the application prospect is wide.

Description

technical field [0001] The invention belongs to the fields of environmental bioengineering and water treatment, and in particular relates to a denitrification and denitrification composite bacterial agent and a preparation method and application thereof. Background technique [0002] With the rapid development of China's economy, a large amount of pollutants such as nitrogen and phosphorus are discharged into water bodies, causing serious eutrophication of water bodies, which has become one of the reasons that threaten the living environment of human beings. Nitrogen pollution in water bodies in China is most serious in the form of nitrate nitrogen and nitrite nitrogen, and biological nitrogen removal is one of the most effective methods to remove nitrogen pollution in water bodies. [0003] Biological denitrification technology uses microbial denitrification to remove pollutants such as nitrate nitrogen and nitrite nitrogen in wastewater. At present, there are problems such...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/025C02F101/16
CPCC02F3/34C02F2101/163C12N1/20
Inventor 杨革刘胜格车程川巩志金刘金锋安笑迟
Owner 河南中顺环保科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com