High-tanshinone salvia miltiorrhiza cultivation method
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A technology of high tanshinone and cultivation method, applied in cultivation, horticultural methods, fertilization methods and other directions, can solve the problems of low added value, inability to meet production and pharmaceutical enterprises, substandard tanshinone content detection, etc.
Inactive Publication Date: 2017-12-22
SHANGHAI TRADITIONAL CHINESE MEDICINE CO LTD +2
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Problems solved by technology
[0004] The technical problem solved by the present invention is to overcome the defects in the prior art such as high planting cost of Salvia miltiorrhiza, low added value, substandard detection of ta...
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preparation example Construction
[0067] 2. Preparation of reference solution
[0068] Tanshinone II A Take an appropriate amount of the reference substance, weigh it accurately, put it in a brown measuring bottle, add methanol to make a solution containing 20 μg per 1 ml, and get it.
[0069] 3. Preparation of the test solution
[0070] Take about 0.3g of the sample powder (through the No. 3 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50ml of methanol precisely, seal it tightly, weigh it, ultrasonically treat it (power 140W, frequency 42kHz) for 30min, and let it cool , and then weigh the weight, make up the lost weight with methanol, shake well, filter, and take the filtrate, that is.
[0071] 4. Determination method
[0072] Precisely draw 10 μl each of the reference substance solution and the test solution, and inject them into the liquid chromatograph. Tanshinone II A The reference substance is used as a reference, and the corresponding peak is the S peak to calculate the...
Embodiment 1
[0077] (1) Soil preparation
[0078] Reddish-brown silt loam (sand content 55%-65%) with deep soil layer (>1m), sunny terrain, good drainage, slightly alkaline, and medium fertility was selected as the planting base and seedling raising base of Salvia miltiorrhiza with high tanshinones. Choose wheat, corn, and non-rhizome traditional Chinese medicinal materials as the previous crops in the planting base and seedling raising base.
[0079] The base should be prepared after the previous crops are harvested, with a deep plow of 40cm. Apply per mu (N: P 2 o 5 :K 2 O=20:10:15) 50kg of compound fertilizer is used as the base fertilizer for the high tanshinone planting base of Salvia miltiorrhiza, the ratio of nitrogen, phosphorus and potassium is more critical, because high nitrogen levels can promote the growth of the aboveground part of Salvia miltiorrhiza, reduce the root-to-shoot ratio, and provide a better foundation for the later stage. Water and fertilizer regulation prov...
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Abstract
The invention discloses a high-tanshinone salvia miltiorrhiza cultivation method. The method includes steps: (1) land preparation; (2) transplanting, namely subjecting seedlings obtained after seedling culture to transplanting pretreatment including (a) seedling selection, (b) root truncating treatment, (c) secondary airing and (d) root dipping treatment and seedling planting; (3) strong seedling cultivation; (4) topping and defloration; (5) water and fertilization regulation; (6) harvesting. By adoption of the high-tanshinone salvia miltiorrhiza cultivation method, salvia miltiorrhiza yield can be increased to keep yield per mu above 300kg while the tanshinone content of salvia miltiorrhiza is increased, the content of tanshinone in a salvia miltiorrhiza root system reaches 0.55%-0.75% and is much higher than a standard of 0.25% prescribed in a pharmacopeia, and the content of tanshinone in salvia miltiorrhiza cultivated according to the method can be stably kept in a range of 0.55%-0.75%. The high-tanshinone salvia miltiorrhiza cultivation method has advantages that raw material demands can be guaranteed, production cost of enterprises is reduced, and high economic benefits and social benefits are achieved.
Description
technical field [0001] The invention belongs to the field of cultivation of salvia miltiorrhiza, in particular to a method for cultivating salvia miltiorrhiza with high tanshinone content. Background technique [0002] Salvia miltiorrhiza Bge. is a perennial herbaceous plant of the genus Salvia in the Labiatae family. It is used as medicine with dried roots and rhizomes, and belongs to traditional Chinese medicinal materials. Salvia miltiorrhiza has the effects of promoting blood circulation and removing blood stasis, promoting menstruation and relieving pain, clearing heart and eliminating troubles, cooling blood and eliminating carbuncle, making it occupy an unshakable position in the field of cardiovascular and cerebrovascular diseases, and has a huge annual demand. At present, there are more than 800 major preparation companies in the country producing Danshen series drugs. The main dosage forms are tablets, capsules, dripping pills, soft capsules, pills, freeze-dried po...
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