Pseudomonas otitidis and application of pseudomonas otitidis in degrading feather produced oligopeptide
A technology of Pseudomonas otitidis and feathers, applied in the field of microorganisms, can solve the problems of unfavorable industrial production of livestock and poultry waste recycling, high protein content of macromolecules, backlog of renewable resources, etc., and achieve high content of feather oligopeptides, Excellent feed efficiency and full recycling effect
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Embodiment 1
[0029] Embodiment 1: the screening of bacterial strain
[0030] 1. Enrichment and acclimatization: Take a mixed sample of soil that has accumulated feathers for a long time, fermentation pond residue and sewer sludge, and mix it with water at a mass ratio of 1:5. Get 10ml from it and put it into a 250ml Erlenmeyer flask filled with 80ml No. 1 enrichment medium, cultivate it on a shaker under the conditions of 37°C, pH value 7, and 150r / min until the feathers are basically rotten; then take the enrichment medium in the bottle The liquid was inoculated into the No. 2 enrichment medium at an inoculum amount of 5%, cultured under the same conditions as above until the feathers were basically rotted, and passed twice.
[0031] The composition of the No. 1 enrichment medium is: NH 4 Cl 0.5g, NaCl 0.5g, K 2 HPO 4 0.7g, KH 2 PO 4 0.35g, MgSO 4 ·7H 2 O 0.2g, yeast extract 0.1g, glucose 1g, pH value 7.0, add feather 5g, sterilize at 115℃ for 30min.
[0032] The composition of t...
Embodiment 2
[0039] Embodiment 2: bacterial strain identification
[0040] 1. Observation of colony and bacterial morphology: The purified and isolated Pseudomonas otitidis H11 was cultured by streaking on the beef extract peptone plate medium, and cultured at a constant temperature of 37°C for 48 hours. The colony was reddish-brown and irregular in shape by visual inspection. Regular, the surface is moist and smooth, shiny and opaque, as shown in Figure 1. Observed under a scanning electron microscope, as figure 2 As shown, the bacteria were rod-shaped, 1.09-1.93 μm long, 0.365-0.415 μm wide, without flagella, and the Gram test was negative (such as image 3 shown).
[0041] 2. Observation of culture characteristics: Pseudomonas otitidis H11 was inoculated in beef extract peptone medium with the same inoculum amount, cultured on a shaker at different temperatures for 18 hours, and the strains in the environment of 7-45°C could grow , where the optimal growth temperature is 28°C; cultu...
Embodiment 3
[0043] Example 3: Optimization of fermentation conditions for producing oligopeptides
[0044] 1) Preparation of secondary seed liquid: Pick a ring of Pseudomonas otitidis H11 preserved on a slant, inoculate it into beef extract peptone test tube medium and activate it for 12 hours at 28°C to obtain primary seed liquid, then inoculate the primary seed liquid according to Inoculate 2% of the inoculum into 30ml beef extract peptone medium and incubate at 28°C for 12h to obtain the secondary seed solution.
[0045] The beef extract-peptone culture medium comprises: peptone 10g / L, beef extract 5g / L, sodium chloride 5g / L, pH value is 6.0, sterilized at 121 DEG C for 20min.
[0046] 2), Fermentation and degradation of feathers: the secondary seed liquid of Pseudomonas otitidis H11 was inserted into the fermentation medium at an inoculation amount of 2%, and the degradation of feathers by different fermentation pH, fermentation temperature and fermentation cycle was investigated by s...
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