Method for determining mouse body components based on low field nuclear magnetic resonance

Abstract

The invention discloses a method for determining mouse body components based on low field nuclear magnetic resonance. The method comprises the following steps: (1) preparing standard samples of mouse body components, namely, muscles, fat and water; (2) testing the standard samples, performing constant-temperature processing on simulated standard samples, testing nuclear magnetic resonance signals of each standard sample through a body fat test pulse sequence, and extracting effective data; (3) establishing models, and establishing linear regression equations of the muscles, the fat and the water according to effective data points of the three standard samples and quality data of each standard sample; (4) marking a line and correcting, namely, testing a mixed sample by adopting a test mark line, acquiring the correlativity between test values and real values according to a method of solving an optimal solution, and correcting the models; (5) testing the samples, namely, testing nuclear magnetic resonance data of a mouse to be tested, and analyzing the test data by utilizing the corrected linear regression equations to obtain preset values of the muscles, the fat and the water of a mouse body to be tested. According to the method provided by the invention, the mouse body components can be determined quickly in a waking state of the mouse.

Description

technical field [0001] The invention relates to the field of animal body composition detection, in particular to a method for measuring mouse body composition based on low-field nuclear magnetic resonance. Background technique [0002] At present, the test of small animal muscle and fat content can reflect the health status of the animal, especially the research on obesity is very valuable, such as obesity and eating, obesity and genes; the existing animal body composition test method has CCA (whole body chemical composition) analysis), BIA (bioelectrical impedance analysis) and DXA (dual energy X-ray absorptiometry), computed tomography and magnetic resonance imaging methods, etc. Whole-body chemical composition analysis method has accurate test results, but it requires the use of chemical reagents for fat extraction, which takes a long time and requires the animals to be killed. The principle of bioelectrical impedance analysis is that fat is basically non-conductive. The...

AI Technical Summary

Problems solved by technology

Dual-energy X-ray absorptiometry, X-rays have different attenuation rates when passing through bones, lean tissue (LTM), and fat tissue (FTM) with different densities, and then calculate the need for anesthesia, which takes a long time and is harmful to humans and animals

[0003] These methods require anesthesia or sedation of the animal to keep the experimental animal absolutely still, but anesthesia or sedation will cause side effects such as hypothermia of the animal, and there is a risk of death

There are also methods such as isotope dilution and whole-body conductivity, which are less accurate and cannot track changes in body composition

Images