Microfluidic chip for nucleic acid amplification detection

Abstract

The invention discloses a microfluidic chip for nucleic acid amplification detection. The microfluidic chip comprises three layers of sealed and bonded membranes, wherein the three layers of membranesare an upper membrane, a middle membrane and a bottom membrane from top to bottom sequentially; a sample adding main flow passage I, a sample adding main flow passage II, an exhaust main flow passageI, an exhaust main flow passage II, reaction chambers and exhaust one-way valves with the same number as that of the reaction chambers are arranged on the bottom membrane; a sample adding hole I, anexhaust hole I, sample separating flow passages, overflow flow passages and exhaust flow diversion passages are arranged on the upper membrane; the middle membrane comprises a sample adding hole II, an exhaust hole II, sample adding one-way valves, sample adding guide holes, overflow guide holes I, overflow guide holes II, exhaust guide holes and ventilating water blocking filter elements. According to the microfluidic chip, nucleic acid amplification detection of a plurality of target genes can be performed at the same time, the reaction chambers are not mutually contaminated, nucleic acid amplification can be performed independently without interference, and leakage of amplification products is avoided after detection is completed. Besides, the chip can adopt fluorescence detection, andcan realize qualitative and quantitative analysis.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip for nucleic acid amplification and detection. Background technique [0002] Polymerase chain reaction is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase a small amount of DNA. PCR (Polymerase Chain Reaction) uses DNA denaturation in vitro at a high temperature of 95°C to become a single strand, and at a low temperature (often around 60°C), primers combine with the single strand according to the principle of complementary base pairing, and then adjust When the temperature reaches the optimum reaction temperature of DNA polymerase (about 72°C), DNA polymerase synthesizes a complementary strand along the direction from phosphate to five-carbon sugar (5'-3'). [0003] Generally, the PCR reaction is carried out in a...

AI Technical Summary

Problems solved by technology

[0006] Existing microfluidic chip nucleic acid amplification detection chips cannot solve the problem of nucleic acid amplification and detection of multiple target genes at the same time, and most of them have a two-layer chip structure, which can only perform a single PCR reaction

When multiple reactions are to be performed, multiple chips can only be used in parallel, which causes a great waste of reagent energy

Images