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A porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation

A technology of porcine pancreatic fat and biodiesel, applied in the direction of biofuel, hydrolase, enzyme stabilization, etc., can solve the problems of non-recyclable use, waste of enzyme tolerance, high price, etc., achieve good reuse rate and improve enzyme tolerance performance, the effect of retaining enzyme activity

Inactive Publication Date: 2018-01-16
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the problems of complex operation, pollution, waste in the use of traditional catalysts, low enzyme tolerance, high price and inability to recycle in enzyme catalysis, the present invention provides a novel biomimetic mineralization method. Porcine pancreatic lipase-inorganic hybrid nanoflower catalyst and its preparation method, the method avoids the use of organic reagents in the preparation of nanocatalysts, so it can not only preserve the activity of the enzyme well, but also improve the tolerance of the enzyme

Method used

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  • A porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation
  • A porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation
  • A porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation

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Embodiment 1

[0031] Preparation of porcine pancreatic lipase-inorganic hybrid nanoflower catalyst: using biomimetic mineralization method, the specific steps are as follows: 1) first use copper sulfate pentahydrate and deionized water to prepare a 200mM copper sulfate aqueous solution; 2) mix 1.44g Na 2 HPO 4 , 0.24g KH 2 PO 4 , 0.2g KCl, and 8.0g NaCl were dissolved in 100mL deionized water, then the volume was adjusted to 1L with deionized water, and then adjusted to pH=7.4 with 1M HCl and NaOH to obtain a PBS buffer solution; 3) Weigh 200mg porcine pancreatic lipase Dissolve in 100mL of the above PBS buffer solution, centrifuge at 6000rad / s, pour the resulting supernatant into a 100mL jar; pipette 3mL of the above CuSO 4Add the aqueous solution into a jar and incubate at 4°C for 24 hours; 4) Centrifuge the reaction solution at 8000 rad / s, wash the centrifuged product 4 times with deionized water, and vacuum freeze-dry the precipitate at -50°C to obtain 260 mg of porcine pancreatic fat...

Embodiment 2

[0038] Preparation of porcine pancreatic lipase-inorganic hybrid nanoflower catalyst: preparation of porcine pancreatic lipase-inorganic hybrid nanoflower catalyst: using biomimetic mineralization method, the specific steps are as follows: 1) first use copper sulfate pentahydrate and deionized water to prepare Concentration is 100mM copper sulfate aqueous solution; 2) 1.44g Na 2 HPO 4 , 0.24g KH 2 PO 4 , 0.2g KCl, and 8.0g NaCl were dissolved in 100mL deionized water, then the volume was adjusted to 1L with deionized water, and then adjusted to pH=7.4 with 1M HCl and NaOH to obtain a PBS buffer solution; 3) Weigh 10mg porcine pancreatic lipase Dissolve in 100mL of the above PBS buffer solution, centrifuge at 6000rad / s, pour the resulting supernatant into a 100mL jar; pipette 3mL of the above CuSO 4 Add the aqueous solution into a jar and incubate at 4°C for 24 hours; 4) Centrifuge the reaction solution at 8000 rad / s, wash the centrifuged product 4 times with deionized water...

Embodiment 3

[0041] Preparation of porcine pancreatic lipase-inorganic hybrid nanoflower catalyst: preparation of porcine pancreatic lipase-inorganic hybrid nanoflower catalyst: using biomimetic mineralization method, the specific steps are as follows: 1) first use copper sulfate pentahydrate and deionized water to prepare Concentration is 200mM copper sulfate aqueous solution; 2) 1.44g Na 2 HPO 4 , 0.24g KH 2 PO 4 , 0.2g KCl, and 8.0g NaCl were dissolved in 100mL deionized water, then the volume was adjusted to 1L with deionized water, and then adjusted to pH=7.4 with 1M HCl and NaOH to obtain a PBS buffer solution; 3) Weigh 200mg porcine pancreatic lipase Dissolve in 100mL of the above PBS buffer solution, centrifuge at 6000rad / s, pour the resulting supernatant into a 100mL jar; pipette 3mL of the above CuSO4 aqueous solution into the jar, and incubate at 4°C for 24h; 4) The reaction The solution was centrifuged at 8000 rad / s, the centrifuged product was washed 4 times with deionized ...

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Abstract

The invention discloses a porcine pancreatic lipase-inorganic hybrid nanoflower catalyst, a preparing method thereof, and applications of the catalyst in biodiesel preparation, and belongs to the technical field of biological energy. The method includes adding 1-5 mL of an aqueous CuSO4 solution having a concentration of 80-300 mM into 100 mL of a PBS buffer solution the pH of which is 6-8 and into which 10-250 mg of porcine pancreatic lipase is dissolved; performing incubation at 0-30 DEG C for 24-72 h; then performing centrifugal separation; washing an centrifugation product with deionized water for 3-5 times; freeze-drying the product to obtain the catalyst; and storing the catalyst at -20 DGE C. The weight percentage of the porcine pancreatic lipase in the catalyst is 12-31%. Accordingto the method, no organic agent is used in a nanometer catalyst preparing stage, thus maintaining enzyme activity well and improving enzyme tolerance. In addition, the novel catalyst can avoid a complex separation process in biodiesel synthesis, and has a good reutilization efficiency.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and specifically relates to a porcine pancreatic lipase-inorganic hybrid nanoflower catalyst prepared by a biomimetic mineralization method, a preparation method and its application in the preparation of biodiesel. The catalyst material can effectively catalyze sunflower seeds The oil is converted into biodiesel and has a good recycling rate. Background technique [0002] Biodiesel is a kind of fatty acid alkyl ester based on animal fat or vegetable oil, which is considered as an eco-friendly and renewable energy source due to its good renewability, biodegradability and low emission of environmental pollution, which can be produced from fatty acid Esterification or esterification of oils and fats with short-chain alcohols. Biodiesel production methods, including acid or base catalytic conversion and so on. The acid-catalyzed reaction is suitable for catalyzing glycerol containing a large amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N9/20C12P7/64
CPCY02E50/10
Inventor 汤钧姜伟丁一
Owner JILIN UNIV
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