Preparation method and application of photoelectrochemical biosensor based on iridium complex

A biosensor, iridium complex technology, used in chemical instruments and methods, organic chemistry, scientific instruments, etc., can solve the problems of light damage, weak absorption, and excitation wavelengths limited to the ultraviolet region of biological systems, and achieve high specificity. Analysis of the effect of detection
CN107589162AActive Publication Date: 2018-01-16QINGDAO UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO UNIV OF SCI & TECH
Publication Date
2018-01-16

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Abstract

The invention discloses a preparation method of a photoelectrochemical biosensor based on a cyclometalated iridium complex and application of the photoelectrochemical biosensor to thrombin detection.The biosensor uses the visible light-excited iridium complex as a photoelectrically active material, and the iridium complex is located on colloidal gold to prepare a gold nanometer signal probe. Capturing hairpin DNAs are immobilized on a modified ITO electrode to prepare a working electrode of the biosensor. A thrombin recognition system includes a specific recognition hairpin HP1 and an adjuvant hairpin HP2; in the presence of thrombin, a large number of secondary targets T-DNAs are released under the action of the excision enzyme ExoIII and hybridize with the capturing hairpin DNAs on theworking electrode so as to capture the gold nanometer signal probe, thereby realizing response to photocurrent signals; thrombin with different concentrations allows the amount of the signal probe connected to the working electrode to be different, so photocurrent intensities are different, and thus, quantitative detection of thrombin is realized. The method has the advantages of high sensitivityand good selectivity.
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Description

technical field

[0001] The invention relates to a preparation method of a photoelectrochemical biosensor based on a ring metal iridium complex and its application in thrombin detection, and belongs to the technical field of photoelectric functional materials and biological analysis. Background technique

[0002] Biomarkers, such as microRNA, proteins, enzymes, etc., are a class of markers related to cell growth, proliferation, and disease occurrence that have been proposed in recent years with the development of immunology, molecular biology, and genomics technologies. , Play an important role in early disease diagnosis, prevention, drug target determination, drug response and other aspects, especially in the prevention and control of chronic and complex diseases such as tumors, cardiovascular diseases, and diabetes. As an important biomarker, thrombin can regulate physiological and pathological processes such as inflammation, anemia and wound healing speed, and its concentr...

Claims

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