Preparation method of 5'-adenylic acid

A technology of adenosine acid and adenosine, applied in the field of biochemical industry, can solve problems such as complex process, low yield, unstable process, etc., and achieve the effect of simplified operation steps, high conversion rate of raw materials, and wide source of raw materials

Active Publication Date: 2018-01-19
江苏香地化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the first method can obtain four products at one time, except for adenosine, the other three become by-products
Moreover, it is limited by the source of RNA, and the separation operation in the production process is complicated, and the yield is low; the second method, the process is complicated, produces more three wastes, and the process is unstable and greatly affected by the strains, so it is not suitable for industrialization
At present, the chemical method is mainly used to synthesize 5'-adenylic acid in industry. The main disadvantage of this method is that the process conditions are harsh, there are more three wastes, it corrodes equipment, and it is not conducive to the health of personnel and industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for preparing 5'-adenylic acid, using powdered adenosine and polyphosphoric acid as raw materials, performing a synthesis reaction under solvent-free conditions, the mass ratio of powdered adenosine, polyphosphoric acid and pure water is 1 :3:3, the specific operation steps are as follows:

[0017] Add 100g of powdered adenosine and 300g of polyphosphoric acid into the reactor, stir and mix evenly, control the temperature at 30-35°C, and react for 6 hours. The adenosine disappears as detected by liquid chromatography. After the reaction is complete, add 300g of pure water, slowly raise the temperature to 60°C, keep stirring for 3 hours, quickly cool down to 0~5°C after the heat preservation is completed, adjust the pH of the aqueous solution to 2.5, keep stirring for 3 hours, and filter to obtain 5'-adenylic acid The crude product has a moisture weight of 128.3g, and is refined to obtain the finished product of 5'-adenylic acid, with a net weight of 117.59g, a ...

Embodiment 2

[0019] A method for preparing 5'-adenylic acid, using powdered adenosine and polyphosphoric acid as raw materials, performing a synthesis reaction under solvent-free conditions, the mass ratio of powdered adenosine, polyphosphoric acid and pure water is 1 :3:3, the specific operation steps are as follows:

[0020] Add 100g of powdered adenosine and 300g of polyphosphoric acid into the reactor, stir and mix evenly, control the temperature at 30-35°C, react for 3 hours, and the adenosine disappears as detected by liquid chromatography. After the reaction is complete, add 300g of pure water, slowly raise the temperature to 55°C, keep stirring for 1 hour, quickly cool down to 0~5°C after the heat preservation is completed, adjust the pH of the aqueous solution to 2, keep stirring for 4 hours, and filter to obtain 5'-adenylic acid The crude product has a wet weight of 129.1g, and is refined to obtain the finished product of 5'-adenylic acid, with a net weight of 118.13g, a yield of...

Embodiment 3

[0022] A method for preparing 5'-adenylic acid, using powdered adenosine and polyphosphoric acid as raw materials, performing a synthesis reaction under solvent-free conditions, the mass ratio of powdered adenosine, polyphosphoric acid and pure water is 1 :3:3, the specific operation steps are as follows:

[0023] Add 200g of powdered adenosine and 600g of polyphosphoric acid into the reactor, stir and mix evenly, control the temperature at 30-35°C, and react for 8 hours, and the adenosine disappears as detected by liquid chromatography. After the reaction is complete, add 600g of pure water, slowly raise the temperature to 60°C, keep stirring for 3 hours, quickly cool down to 0~5°C after the heat preservation is completed, adjust the pH of the aqueous solution to 2.5, keep stirring for 3 hours, and filter to obtain 5'-adenylic acid The crude product has a tidal weight of 260.2g, and is refined to obtain the finished product of 5'-adenylic acid, with a net weight of 236.47g, a...

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PUM

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Abstract

The invention discloses a preparation method of 5'-adenylic acid. The preparation method of 5'-adenylic acid comprises the following steps: performing synthesis reaction on powdered adenosine and polyphosphoric acid which serve as raw materials under the condition of no solvent, adding pure water after the synthesis reaction to perform hydrolysis, and refining the crude product obtained by the hydrolysis, wherein the mass ratio of the powdered adenosine to the polyphosphoric acid to the pure water is 1:(2-4):(2-4). The preparation method of 5'-adenylic acid has the following advantages: the solvent-free synthesis method is adopted, the reaction impurities are few, the condition is mild and pollution is avoided; the product yield is 90 percent or above, the product content is more than 99 percent and the conversion rate of the raw materials is high; and the operation steps are simple, the raw materials are wide in source and the price is moderate, so the preparation method of 5'-adenylic acid can be popularized and implemented on a large scale.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a preparation method of 5'-adenylic acid. Background technique [0002] 5'-Adenylic acid is a compound of adenine plus ribose and phosphoric acid. It is one of the four main mononucleotides that constitute ribose in animal cells, and is an energy transfer substance in the body. It has significant peripheral vasodilation and antihypertensive effects. Industrially, 5'-adenylic acid can be used as an intermediate in the production of nucleic acid drugs, health food and biochemical reagents, and used to manufacture biochemical drugs such as adenosine triphosphate (ATP) and cyclic adenosine monophosphate (cAMP). [0003] There are three main production methods for 5'-adenylic acid, 1) degraded from RNA (ribonucleic acid), and then separated; 2) biological fermentation method, adenosine is fermented by bacteria to generate adenylic acid, and then separated Obtained; 3) Starting fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/20C07H1/02
Inventor 张剑刘拥军于广张玮琪张军
Owner 江苏香地化学有限公司
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