Preparation method of 5'-adenylic acid

Abstract

The invention discloses a preparation method of 5'-adenylic acid. The preparation method of 5'-adenylic acid comprises the following steps: performing synthesis reaction on powdered adenosine and polyphosphoric acid which serve as raw materials under the condition of no solvent, adding pure water after the synthesis reaction to perform hydrolysis, and refining the crude product obtained by the hydrolysis, wherein the mass ratio of the powdered adenosine to the polyphosphoric acid to the pure water is 1:(2-4):(2-4). The preparation method of 5'-adenylic acid has the following advantages: the solvent-free synthesis method is adopted, the reaction impurities are few, the condition is mild and pollution is avoided; the product yield is 90 percent or above, the product content is more than 99 percent and the conversion rate of the raw materials is high; and the operation steps are simple, the raw materials are wide in source and the price is moderate, so the preparation method of 5'-adenylic acid can be popularized and implemented on a large scale.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a preparation method of 5'-adenylic acid. Background technique [0002] 5'-Adenylic acid is a compound of adenine plus ribose and phosphoric acid. It is one of the four main mononucleotides that constitute ribose in animal cells, and is an energy transfer substance in the body. It has significant peripheral vasodilation and antihypertensive effects. Industrially, 5'-adenylic acid can be used as an intermediate in the production of nucleic acid drugs, health food and biochemical reagents, and used to manufacture biochemical drugs such as adenosine triphosphate (ATP) and cyclic adenosine monophosphate (cAMP). [0003] There are three main production methods for 5'-adenylic acid, 1) degraded from RNA (ribonucleic acid), and then separated; 2) biological fermentation method, adenosine is fermented by bacteria to generate adenylic acid, and then separated Obtained; 3) Starting fr...

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Problems solved by technology

Although the first method can obtain four products at one time, except for adenosine, the other three become by-products

Moreover, it is limited by the source of RNA, and the separation operation in the production process is complicated, and the yield is low; the second method, the process is complicated, produces more three wastes, and the process is unstable and greatly affected by the strains, so it is not suitable for industrialization

At present, the chemical method is mainly used to synthesize 5'-adenylic acid in industry. The main disadvantage of this method is that the process conditions are harsh, there are more three wastes, it corrodes equipment, and it is not conducive to the health of personnel and industrial production.