Lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders and application of lipid-decreasing pentapeptide

A blood lipid-lowering and swim bladder technology, applied in the field of oligopeptides, can solve problems such as insufficient basic research on swim bladders, achieve good lipid-lowering activity, reduce lipid production, and promote the effect of fatty acid β-oxidation

Inactive Publication Date: 2018-01-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on foreign studies using swim bladders as raw materials, and the basic research on swim bladders is obviously insufficient
On the other hand, most of the lipid-lowering substances from marine sources reported in existing studies are concentrated in po

Method used

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  • Lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders and application of lipid-decreasing pentapeptide
  • Lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders and application of lipid-decreasing pentapeptide
  • Lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders and application of lipid-decreasing pentapeptide

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Effect test

Embodiment 1

[0031] A lipid-lowering pentapeptide derived from the fish swim bladder. The amino acid sequence of the lipid-lowering pentapeptide is: Gly-Ile-Glu-Trp-Ala (GIEWA). The molecular weight determined by ESI-MS is 574.64Da. As an active ingredient for lowering blood lipids, health products and food additives; the preparation method of pentapeptide lowering blood lipids includes: pretreatment, enzymatic hydrolysis, and extraction, specifically including the following steps:

[0032] Pretreatment: After cleaning and homogenizing the swim bladder of Miichthysmiiuy to a paste, add 0.1mol / L NaOH and soak for 12 hours at 4°C according to the material-to-liquid ratio of 1g:10mL, filter, and wash with distilled water until it is neutral. Ratio 1g: add 10% isopropanol solution to 5mL, degrease for 36 hours, filter, rinse the solids with distilled water with isopropanol, and dry to obtain defatted fish maw; use alkaline extraction followed by alcohol degreasing to remove the fish Impurities in...

Embodiment 2

[0036] The preparation process of the blood lipid-lowering pentapeptide of Owl fish swim bladder is as follows: The preparation of the blood lipid-lowering pentapeptide of the owl fish swim bladder "Delipidation" enzymatic hydrolysis, "Ultrafiltration" Ion exchange chromatography, Gel filtration chromatography, High performance liquid chromatography "Functional evaluation.

[0037] The specific steps are:

[0038] 1) Pretreatment of the maw bladder: After cleaning and homogenizing the maw of Miichthysmiiuy to a paste, add 0.1mol / L NaOH to 1g:10mL and soak for 15h at 4°C, filter, and wash with distilled water until it is neutral, according to 1g :Add 10% isopropanol solution in 5mL, defatted for 48h, filter, and rinse the solid with distilled water with isopropanol, dry, and get defatted fish maw;

[0039] 2) Enzymatic hydrolysis of owl fish maw: Take defatted owl fish maw and add distilled water according to the material-to-liquid ratio of 1g:5mL. Adjust the pH to 10, add 2.5% alka...

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Abstract

The invention discloses lipid-decreasing pentapeptide derived from Miichthysmiiuy swimming bladders. The amino acid sequence of the lipid-decreasing pentapeptide is Gly-Ile-Glu-Trp-Ala(GIEWA), and themolecular weight determined by virtue of ESI-MS is 574.64Da. The lipid-decreasing pentapeptide is prepared by the steps of processing the Miichthysmiiuy swimming bladders by virtue of alkali leachingand alcohol degreasing so as to obtain degreased Miichthysmiiuy swimming bladders, carrying out enzymolysis by virtue of alkaline protease, and carrying out ultrafiltration, ion exchange resin chromatography, gel column chromatography and reversed-phase high performance liquid chromatography purification on enzymatic hydrolysate, so as to obtain the lipid-decreasing pentapeptide derived from theMiichthysmiiuy swimming bladders. The invention simultaneously discloses application of the lipid-decreasing pentapeptide derived from the Miichthysmiiuy swimming bladders in lipid-decreasing active components, health products and food additives. According to the lipid-decreasing pentapeptide derived from the Miichthysmiiuy swimming bladders, adipogenesis genes can be reduced, the oxidation of fatty acid beta can be promoted, the generation of lipids can be promoted, and the pentapeptide has good lipid-decreasing activity, and can be developed into lipid-decreasing drugs, health products and food additives.

Description

Technical field [0001] The present invention relates to the field of oligopeptides, in particular to a lipid-lowering pentapeptide derived from koala fish maw and its application. technical background [0002] Marine fish maw is one of the "Eight Treasures of the Ocean" and is known as the "Marine Ginseng". The main nutrients of fish swim bladder are active ingredients such as advanced collagen, among which the protein content is more than 80%, and the fat content is low. Therefore, fish maw is an ideal high-protein and low-fat food. [0003] my country has a long history of eating fish swim bladder. The performance of fish swim bladder has been recorded in the Northern Wei Dynasty "Qi Min Yao Shu", the Tang Dynasty "New Tang Book·Geography" and Li Shizhen-"Compendium of Materia Medica". Local residents in Zhoushan will use fish maw glue, a dry product of koala fish maw, for children at the developmental stage to improve the child’s immunity and promote development. At present, ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/18C07K1/20A61K38/08A61P3/06A23L33/18
Inventor 王斌赵文浩潘欣赵玉勤
Owner ZHEJIANG OCEAN UNIV
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