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Application of Langduning b in the preparation of drugs for preventing and treating diseases related to nerve cell damage

A nerve cell injury, Langduning technology, applied in the field of medicine, can solve problems such as diseases related to nerve cell injury that have not been seen

Active Publication Date: 2020-03-17
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the Chinese invention patents on Langduning B can only be seen in the preparation method (CN104610214A, CN105315118A, CN106866601A) and the preparation of anticancer drugs (CN103933027A, CN103933029A), and there is no report on the prevention and treatment of nerve cell damage related diseases

Method used

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  • Application of Langduning b in the preparation of drugs for preventing and treating diseases related to nerve cell damage
  • Application of Langduning b in the preparation of drugs for preventing and treating diseases related to nerve cell damage
  • Application of Langduning b in the preparation of drugs for preventing and treating diseases related to nerve cell damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 The protective effect of Langduning B on cerebral ischemia-reperfusion injury in rats

[0033] Rat middle cerebral artery occlusion (MCAO) model preparation:

[0034] The middle cerebral artery infarction model in rats was established by suture method. Rats were fasted for 12 h before the operation. 1% sodium pentobarbital (50mg·kg -1 ) to anesthetize the rats by intraperitoneal injection, fix the rats in the supine position on the operating board, cut an opening about 1.5 cm in the middle of the neck, separate and expose the right common carotid artery (CCA), look for and carefully separate the internal carotid artery (CCA) along the CCA ( ICA) and external carotid artery (ECA). Ligate the proximal end of the CCA and the ECA, use a sterile injection needle to make a small opening 6mm away from the bifurcation of the ICA and the CCA, carefully insert the blunt end of the nylon thread into the ICA along the opening, and slowly push forward 18-20mm, so that ...

Embodiment 2

[0045] Example 2 Effect of Langduning B on Oxidative Stress Damage in Brain Tissue of Rats with Cerebral Ischemia-reperfusion

[0046] The brain tissue in the ischemic area was collected, and after the cerebellum, lower brainstem and blood vessels were removed, the brain homogenate was prepared with normal saline, and the relevant indicators of oxidative stress damage in brain tissue were detected.

[0047] Experimental results ( Figure 5 ) showed that compared with the sham-operated rats, the MDA content in the ischemic brain tissue of the transient ischemic rats was significantly increased (P<0.01); compared with the model group, the corresponding brain tissue of the Langduning B administration group The malondialdehyde content decreased significantly (P<0.01).

[0048] Experimental results ( Image 6 ) showed that, compared with sham-operated rats, the level of carbonyl protein in ischemic brain tissue of ischemic rats was significantly increased (P<0.01); The content d...

Embodiment 3

[0049] Example 3 Protective effect of Langduning B on SH-SY5Y nerve cell injury induced by oxidative stress

[0050] SH-SY5Y cells were cultured in complete RMPI1640 medium (containing 10% fetal bovine serum, 100 U / mL penicillin, 100 μg / mL streptomycin, 1% glutamine), at 37°C, 5% CO 2 cultured under saturated humidity conditions. Change the solution every 2-3 days. Take the cells in the logarithmic growth phase, digest with 0.25% trypsin, and dilute the cell concentration to 10 with complete medium 5 cells / mL, inoculated into a 96-well cell culture plate, 100 μL per well, and after 24 hours of cultivation, replaced with 300 μmol / L H 2 o 2 After 24 hours of treatment, the cell viability was measured by MTT method. MTT test results ( Figure 7 ) shows that 300μM H 2 o 2 Acting on SH-SY5Y cells for 24 hours can lead to a significant decline in cell viability (P<0.05), and the number of surviving cells is about 50% of the normal group; and giving wolftoxin B can significant...

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Abstract

The invention discloses a new drug use of chamaejasmenin B, and specifically discloses a use of the chamaejasmenin B in preparation of a drug for prevention and treatment of neuronal cell damage related diseases such as cerebral ischemic diseases, cerebral ischemia reperfusion injury, oxidative stress brain injury, cerebral ischemia sequelae and the like. The chamaejasmenin B is isolated from an ethanol extract of the root of thymelaeaceae stellera stellera chamaejasme l., and a structure is characterized in that two dihydroflavones are connected with each other through a C-3 site carbon-carbon single bond to form dual-dihydroflavone. The compound is found out to have significant protective effect in ischemic brain injury in drug activity evaluation, and can be used as the drug for resisting nerve cell damage related diseases such as cerebral ischemia in clinical use.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of Langduning B in the preparation and treatment of diseases related to nerve cell damage including cerebral ischemic disease, cerebral ischemia-reperfusion injury, and oxidative stress brain injury. [0002] technical background [0003] Nerve cell injury-related diseases are represented by cerebrovascular diseases, including cerebral ischemic diseases, cerebral ischemia-reperfusion injury, oxidative stress brain injury, and sequelae of cerebral ischemia. Cerebrovascular disease is characterized by high morbidity, high mortality, high disability and high recurrence rate, and is one of the main diseases that threaten the health of the population. Stroke, as its representative, is the most important fatal disease in the world One of them, ischemic stroke patients account for 60-80% of the total stroke patients. [0004] Ischemic stroke refers to the bra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K45/06A61K36/16A61P9/10A61P25/00
Inventor 续洁琨张维库贺晓丽赫军刘淑娜
Owner BEIJING UNIV OF CHINESE MEDICINE
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